Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   D16iCDA_RS08305 Genome accession   NZ_CP076114
Coordinates   1780995..1782587 (+) Length   530 a.a.
NCBI ID   WP_070880616.1    Uniprot ID   -
Organism   Phytopseudomonas seleniipraecipitans strain D1-6     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1775995..1787587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D16iCDA_RS08285 (D16iCDA_08285) pgeF 1777751..1778491 (-) 741 WP_070880619.1 peptidoglycan editing factor PgeF -
  D16iCDA_RS08290 (D16iCDA_08290) rluD 1778488..1779462 (-) 975 WP_070880797.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  D16iCDA_RS08295 (D16iCDA_08295) - 1779610..1780599 (+) 990 WP_070880618.1 outer membrane protein assembly factor BamD -
  D16iCDA_RS08300 (D16iCDA_08300) - 1780763..1781005 (+) 243 WP_070880617.1 PP0621 family protein -
  D16iCDA_RS08305 (D16iCDA_08305) pilS 1780995..1782587 (+) 1593 WP_070880616.1 sensor histidine kinase Regulator
  D16iCDA_RS08310 (D16iCDA_08310) pilR 1782592..1783929 (+) 1338 WP_070880615.1 sigma-54-dependent transcriptional regulator Regulator
  D16iCDA_RS08315 (D16iCDA_08315) - 1784018..1785079 (-) 1062 WP_070880796.1 FAD-dependent oxidoreductase -
  D16iCDA_RS08320 (D16iCDA_08320) - 1785225..1785716 (+) 492 WP_070880795.1 GspH/FimT family pseudopilin -
  D16iCDA_RS21145 (D16iCDA_08325) - 1785819..1786331 (+) 513 WP_070880614.1 GspH/FimT family pseudopilin -
  D16iCDA_RS08335 (D16iCDA_08330) pilV 1786345..1786911 (+) 567 WP_070880613.1 type IV pilus modification protein PilV -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58562.05 Da        Isoelectric Point: 9.0062

>NTDB_id=571978 D16iCDA_RS08305 WP_070880616.1 1780995..1782587(+) (pilS) [Phytopseudomonas seleniipraecipitans strain D1-6]
MPTEALALSGLQGRRVFRLYHLYRLLIGVLLVLLISAELDAELLQLAHAPLFRGASWLYLILNIFIALIIHNPRSLIQVF
GVALVDVILLSALFYAGGGTPSGIGNLLIVAVAIANILLRGRIGVLIAASAAIGLIYLTFYLSLNNPQASSQYVQAGALG
ALCFAAALFVQGVTRRLQASEILAEQRAADVADLEALNNLILQRMRTGILVVDEQHRVLLANQAALSLLGREHLAGKILD
PHCTELVKRLQHWQENSSLRPASLQARVDGPTLQPSFIALQRGGQRHTLILLDDISQIAQQAQQLKLVALGRLTAGIAHE
IRNPLGAISHAAQLLQESEDLQGPDLRLTQIIQDQSRRMNLIIENVLQLSRRRQAEPQLLDLKYWLHRFAAEFRTTAPAS
RMLHLAIEGSSIQTRMDPNQLTQVITNLVQNGLRYSAQLNPKGQVWLKLFRDPRSDLPVLEVLDDGPGVPTDQLHHIFEP
FYTTENKGTGLGLYISRELCESNQAHLHYLPREGGGACFRITFAHPRKMS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=571978 D16iCDA_RS08305 WP_070880616.1 1780995..1782587(+) (pilS) [Phytopseudomonas seleniipraecipitans strain D1-6]
GTGCCGACTGAAGCGCTCGCGTTAAGCGGCCTGCAAGGGCGGCGTGTTTTCCGGCTATACCACCTCTACAGATTGCTTAT
CGGCGTTCTGCTGGTCCTGCTGATCTCGGCAGAACTGGATGCGGAACTGCTGCAGCTGGCCCACGCGCCATTGTTTCGAG
GCGCCAGCTGGCTGTACCTGATCCTCAATATTTTCATCGCCCTGATCATCCACAACCCGCGCAGCCTGATACAGGTATTC
GGCGTGGCGCTGGTCGATGTGATTCTTCTCTCGGCTTTGTTCTACGCAGGCGGCGGCACTCCTAGCGGTATCGGCAACCT
GCTGATCGTGGCGGTGGCGATTGCCAACATCCTGCTGCGCGGTCGCATCGGCGTGCTGATCGCCGCCAGTGCGGCCATTG
GCCTGATCTATCTGACCTTCTACCTCAGCCTCAACAATCCCCAAGCCAGCAGCCAGTATGTTCAAGCCGGCGCTCTGGGT
GCGCTGTGCTTCGCCGCCGCGCTGTTCGTACAGGGCGTGACGCGGCGCCTGCAAGCCAGTGAAATTCTCGCCGAGCAGCG
CGCTGCCGATGTAGCCGACCTTGAAGCGCTGAACAACCTGATTCTGCAGCGCATGCGCACCGGTATCCTGGTCGTCGACG
AACAGCATCGCGTGCTGCTGGCCAACCAGGCGGCCCTCAGCCTGCTTGGCCGCGAACACCTGGCCGGCAAAATTCTCGAC
CCGCATTGCACCGAACTGGTAAAGCGGCTGCAGCACTGGCAGGAGAACTCCAGCCTGCGCCCGGCGAGCCTCCAGGCGCG
GGTCGATGGCCCAACCCTGCAACCCAGCTTTATCGCCTTGCAGCGAGGTGGCCAGCGCCACACGCTGATCCTGCTCGACG
ACATTTCGCAGATCGCCCAGCAAGCCCAGCAACTCAAGCTGGTCGCCCTCGGCCGCCTGACTGCAGGCATCGCCCATGAG
ATTCGCAACCCGCTGGGCGCCATCAGTCATGCCGCCCAGTTGCTGCAGGAGTCCGAAGACCTGCAGGGGCCAGACCTGCG
TCTGACTCAGATCATCCAGGATCAATCCAGGCGCATGAACCTGATCATCGAGAACGTCCTGCAGCTGTCACGCCGTCGCC
AGGCGGAGCCGCAACTGCTCGATCTCAAATACTGGCTGCATCGCTTCGCGGCAGAATTTCGCACCACTGCGCCGGCCTCG
CGAATGCTCCACCTGGCTATCGAAGGCAGCAGCATCCAGACCCGCATGGACCCCAACCAGCTGACCCAGGTAATCACCAA
CCTGGTACAGAACGGCCTGCGTTACAGCGCCCAACTCAATCCTAAGGGCCAGGTATGGCTGAAACTGTTCCGTGACCCGC
GCAGCGATCTGCCGGTACTCGAGGTGCTTGATGATGGCCCGGGCGTGCCGACAGACCAACTGCATCACATCTTCGAGCCT
TTCTACACCACGGAAAACAAAGGCACCGGCCTGGGCCTGTATATTTCCCGCGAGCTTTGCGAAAGCAATCAGGCTCATCT
TCACTACCTACCACGCGAAGGCGGCGGTGCCTGCTTCCGTATCACCTTCGCGCATCCGCGAAAAATGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

PAS domain (PAS_8)

(202-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

65.66

100

0.657