Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   MNG38_RS07740 Genome accession   NZ_CP093289
Coordinates   1461466..1463565 (-) Length   699 a.a.
NCBI ID   WP_015252215.1    Uniprot ID   -
Organism   Bacillus subtilis strain H2     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 1456466..1468565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNG38_RS07710 (MNG38_07710) spo0E 1456520..1456777 (+) 258 WP_003218598.1 aspartyl-phosphate phosphatase Spo0E -
  MNG38_RS07715 (MNG38_07715) - 1456863..1457294 (+) 432 WP_242139224.1 hypothetical protein -
  MNG38_RS07720 (MNG38_07720) kinD 1457322..1458842 (-) 1521 WP_015252216.1 sporulation kinase KinD -
  MNG38_RS07725 (MNG38_07725) mhqR 1459035..1459472 (+) 438 WP_003232475.1 MarR family transcriptional regulator MhqR -
  MNG38_RS07730 (MNG38_07730) motB 1459512..1460297 (-) 786 WP_003232473.1 flagellar motor protein MotB -
  MNG38_RS07735 (MNG38_07735) motA 1460269..1461081 (-) 813 WP_003238984.1 flagellar motor stator protein MotA -
  MNG38_RS07740 (MNG38_07740) clpE 1461466..1463565 (-) 2100 WP_015252215.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  MNG38_RS07745 (MNG38_07745) - 1463931..1464974 (+) 1044 WP_015252214.1 hypothetical protein -
  MNG38_RS07750 (MNG38_07750) queC 1465287..1465946 (+) 660 WP_003245417.1 7-cyano-7-deazaguanine synthase QueC -
  MNG38_RS07755 (MNG38_07755) queD 1465939..1466388 (+) 450 WP_003232462.1 6-carboxytetrahydropterin synthase QueD -
  MNG38_RS07760 (MNG38_07760) queE 1466381..1467112 (+) 732 WP_015252213.1 7-carboxy-7-deazaguanine synthase QueE -
  MNG38_RS07765 (MNG38_07765) queF 1467130..1467627 (+) 498 WP_003218613.1 preQ(1) synthase -
  MNG38_RS07770 (MNG38_07770) ykvN 1468177..1468533 (-) 357 WP_003245808.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77921.07 Da        Isoelectric Point: 5.2425

>NTDB_id=570893 MNG38_RS07740 WP_015252215.1 1461466..1463565(-) (clpE) [Bacillus subtilis strain H2]
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGSQSFGFPFEQAFQPKEKSAAKQSGKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKTKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIIEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAISKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGIRLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=570893 MNG38_RS07740 WP_015252215.1 1461466..1463565(-) (clpE) [Bacillus subtilis strain H2]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTTCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAAAAGAGCGCAGCAAAACAAAGCGGAAAAAAAGGGCTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCTAAAGCCGGTCTCATTGATCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGCAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTGGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGTGATGTTCCAAACAAACTGAAAAACAAAGAGCTATATTTGCTTGATGTTGCATCCCTTGTTGCA
AATACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGCTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTCGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCGTACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCGTGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAACAAAAGCCCTGGAAGAAGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTATTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAGCTTGAAGCAAAACTTCATGAACGCGTGATCGGACAAGAAGCCGCTGTTCAAAAAGTGGCAAAGGCGGTAAGACG
AAGCCGCGCCGGATTAAAATCCAAAAACAGACCAGTCGGCTCCTTCCTCTTCGTAGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCATATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAGAAAGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAAGATGGCCGTCTGACAGACAGCCAAGGCAGAACTGTAAGCTTCAAAGACACAGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTAATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCGGAACGGGGCATTCGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATTGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATTCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGAGCGAAATAA

Domains

Predicted by InterProScan.

AAA lid domain (AAA_lid_9)

(253-352)

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

55.855

100

0.594
  clpC Lactococcus lactis subsp. cremoris KW2

56.011

100

0.587
  clpE Streptococcus pneumoniae TIGR4

55.062

100

0.568
  clpE Streptococcus pneumoniae Rx1

55.062

100

0.568
  clpE Streptococcus pneumoniae D39

55.062

100

0.568
  clpE Streptococcus pneumoniae R6

55.062

100

0.568
  clpC Bacillus subtilis subsp. subtilis str. 168

53.701

90.844

0.488
  clpC Streptococcus pneumoniae Rx1

45.732

93.848

0.429
  clpC Streptococcus pneumoniae D39

45.732

93.848

0.429
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.847

89.7

0.429
  clpC Streptococcus thermophilus LMD-9

45.427

93.848

0.426
  clpC Streptococcus thermophilus LMG 18311

44.97

93.848

0.422
  clpC Streptococcus pneumoniae TIGR4

46.299

90.844

0.421
  clpC Streptococcus mutans UA159

46.216

88.841

0.411
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

42.244

86.695

0.366