Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MNY24_RS18675 Genome accession   NZ_CP093226
Coordinates   3881889..3882329 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain EC-16-35     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3876889..3887329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNY24_RS18655 aroP 3877887..3879260 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  MNY24_RS18660 ampE 3879303..3880157 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  MNY24_RS18665 ampD 3880154..3880705 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MNY24_RS18670 nadC 3880793..3881686 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MNY24_RS18675 pilA 3881889..3882329 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  MNY24_RS18680 pilB 3882339..3883724 (+) 1386 WP_001025146.1 type II secretion system protein GspE Machinery gene
  MNY24_RS18685 hofC 3883714..3884916 (+) 1203 WP_000157266.1 protein transport protein HofC -
  MNY24_RS18690 guaC 3884951..3885994 (-) 1044 WP_001217338.1 GMP reductase -
  MNY24_RS18695 - 3886150..3886194 (-) 45 WP_120795372.1 protein YacM -
  MNY24_RS18700 coaE 3886219..3886839 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=570731 MNY24_RS18675 WP_000360904.1 3881889..3882329(+) (pilA) [Escherichia coli strain EC-16-35]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=570731 MNY24_RS18675 WP_000360904.1 3881889..3882329(+) (pilA) [Escherichia coli strain EC-16-35]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363