Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   MM522_RS05075 Genome accession   NZ_CP093067
Coordinates   955892..957991 (-) Length   699 a.a.
NCBI ID   WP_101172467.1    Uniprot ID   -
Organism   Bacillus subtilis strain ATC-3     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 950892..962991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MM522_RS05045 (MM522_05045) spo0E 950947..951204 (+) 258 WP_003232480.1 aspartyl-phosphate phosphatase Spo0E -
  MM522_RS05050 (MM522_05050) - 951290..951721 (+) 432 WP_003232479.1 hypothetical protein -
  MM522_RS05055 (MM522_05055) kinD 951749..953269 (-) 1521 WP_072557174.1 sporulation kinase KinD -
  MM522_RS05060 (MM522_05060) mhqR 953462..953899 (+) 438 WP_003232475.1 MarR family transcriptional regulator MhqR -
  MM522_RS05065 (MM522_05065) motB 953939..954724 (-) 786 WP_003232473.1 flagellar motor protein MotB -
  MM522_RS05070 (MM522_05070) motA 954696..955508 (-) 813 WP_003238984.1 flagellar motor stator protein MotA -
  MM522_RS05075 (MM522_05075) clpE 955892..957991 (-) 2100 WP_101172467.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  MM522_RS05080 (MM522_05080) - 958357..959400 (+) 1044 WP_021479408.1 membrane protein -
  MM522_RS05085 (MM522_05085) queC 959713..960372 (+) 660 WP_003232465.1 7-cyano-7-deazaguanine synthase QueC -
  MM522_RS05090 (MM522_05090) queD 960365..960814 (+) 450 WP_003232462.1 6-carboxytetrahydropterin synthase QueD -
  MM522_RS05095 (MM522_05095) queE 960807..961538 (+) 732 WP_003232460.1 7-carboxy-7-deazaguanine synthase QueE -
  MM522_RS05100 (MM522_05100) queF 961556..962053 (+) 498 WP_003218613.1 preQ(1) synthase -
  MM522_RS05105 (MM522_05105) ykvN 962635..962964 (-) 330 WP_241495702.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77789.89 Da        Isoelectric Point: 5.1809

>NTDB_id=570388 MM522_RS05075 WP_101172467.1 955892..957991(-) (clpE) [Bacillus subtilis strain ATC-3]
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEQSAAKQSGKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPTIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKTKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQAAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=570388 MM522_RS05075 WP_101172467.1 955892..957991(-) (clpE) [Bacillus subtilis strain ATC-3]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAACAGAGCGCAGCAAAACAAAGCGGAAAAAAAGGGCTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCTAAAGCCGGTCTCATTGATCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGGAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTGGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGTGATGTTCCAAACAAACTGAAAAACAAAGAGCTATATTTGCTTGATGTTGCATCCCTTGTTGCA
AATACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGCTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTCGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTACAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCGTACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCGTGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAACAAAAGCCCTGGAAGAAGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAACTCGAAGCAAAACTTCATGAACGCGTGATTGGACAAGCAGCCGCTGTTCAAAAAGTGGCAAAGGCGGTAAGACG
AAGCCGCGCCGGTTTAAAATCCAAAAACAGGCCAGTCGGCTCCTTCCTCTTCGTCGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAGAAAGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAAGATGGCCGTCTGACAGACAGCCAAGGCAGAACCGTAAGCTTCAAAGACACTGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTAATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCTGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGAGCGAAATAA

Domains

Predicted by InterProScan.

AAA lid domain (AAA_lid_9)

(253-352)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.528

100

0.601
  clpC Lactococcus lactis subsp. cremoris KW2

56.421

100

0.591
  clpE Streptococcus pneumoniae TIGR4

55.062

100

0.568
  clpE Streptococcus pneumoniae Rx1

55.062

100

0.568
  clpE Streptococcus pneumoniae D39

55.062

100

0.568
  clpE Streptococcus pneumoniae R6

55.062

100

0.568
  clpC Bacillus subtilis subsp. subtilis str. 168

53.858

90.844

0.489
  clpC Streptococcus pneumoniae Rx1

45.579

93.848

0.428
  clpC Streptococcus pneumoniae D39

45.579

93.848

0.428
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.528

89.7

0.426
  clpC Streptococcus thermophilus LMD-9

45.274

93.848

0.425
  clpC Streptococcus thermophilus LMG 18311

44.817

93.848

0.421
  clpC Streptococcus pneumoniae TIGR4

46.142

90.844

0.419
  clpC Streptococcus mutans UA159

46.055

88.841

0.409
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

41.749

86.695

0.362