Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MMZ72_RS11760 Genome accession   NZ_CP093021
Coordinates   2491551..2493278 (+) Length   575 a.a.
NCBI ID   WP_241469747.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H09     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2486551..2498278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMZ72_RS11740 (MMZ72_11740) - 2487251..2488180 (-) 930 WP_241469737.1 LysR family transcriptional regulator -
  MMZ72_RS11745 (MMZ72_11745) - 2488203..2489177 (-) 975 WP_241469743.1 quinone oxidoreductase -
  MMZ72_RS11750 (MMZ72_11750) - 2489403..2490155 (+) 753 WP_003110095.1 class I SAM-dependent methyltransferase -
  MMZ72_RS11755 (MMZ72_11755) - 2490758..2491561 (+) 804 WP_241469906.1 ABC transporter permease -
  MMZ72_RS11760 (MMZ72_11760) pilF 2491551..2493278 (+) 1728 WP_241469747.1 GspE/PulE family protein Machinery gene
  MMZ72_RS11765 (MMZ72_11765) - 2493283..2494470 (+) 1188 WP_058139113.1 type II secretion system F family protein -
  MMZ72_RS11770 (MMZ72_11770) gspG 2494524..2494958 (+) 435 WP_024915882.1 type II secretion system major pseudopilin GspG -
  MMZ72_RS11775 (MMZ72_11775) - 2494927..2495337 (+) 411 WP_024915883.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MMZ72_RS11780 (MMZ72_11780) - 2495337..2495762 (+) 426 WP_024915884.1 type II secretion system protein J -
  MMZ72_RS11785 (MMZ72_11785) - 2495759..2496349 (+) 591 WP_024915885.1 type II secretion system protein J -
  MMZ72_RS11790 (MMZ72_11790) - 2496339..2497418 (+) 1080 WP_124099933.1 general secretion pathway protein GspK -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63673.77 Da        Isoelectric Point: 5.0943

>NTDB_id=569668 MMZ72_RS11760 WP_241469747.1 2491551..2493278(+) (pilF) [Pseudomonas aeruginosa strain H09]
MSSEVASPSPQMTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGDDL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSIRETLGYFYPERPIQAVLCRSQDLDGWLEHSLD
LARQGADRQAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLRLDRLGMLPDHLGRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLITVEDPVEYQVPNITQVQVHADIGLTFAAALRSILRQDPDVIMIGEI
RDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAA
TEAATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAW
LGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=569668 MMZ72_RS11760 WP_241469747.1 2491551..2493278(+) (pilF) [Pseudomonas aeruginosa strain H09]
ATGTCCTCTGAGGTTGCTTCCCCCAGCCCGCAGATGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGCCTGGGAGGCATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGCTGGTGGGGGACGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGTACCCCGATCAATACCGACTGGTTCGTCGAAGAGCAGCT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCGCGTGATCCGTTATCTCCCTCGATACGCGAGACCCTGGGCT
ATTTCTATCCGGAGCGCCCGATTCAAGCGGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAACATTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCAAGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGCTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGCTCTTCGGACATTCACATCGAACCCCGGGAAT
TCGACTTCCGTATCCGCTTCCGCGTCGATGGCGTCCTGCACGACCAACTGAGCCTTCCTCGCGAGCGATTCGCGGCGGTC
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCGGGGGTGCATGGCGAATCGGTTGTCATGCGCCTGTTGCCGA
AGGAGCGCGAGGGGCTGCGCCTGGACCGGCTGGGGATGCTCCCGGACCACCTTGGGAGGATGCGCGCATGGGTGGCGGAA
CCCCACGGCATCATCCTGGTTACCGGCCCCACCGGATCGGGCAAGTCCACGACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCACCGTGGAGGACCCCGTGGAGTACCAGGTGCCCAATATCACCCAGGTCCAGGTACATG
CCGATATCGGTCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATA
CGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCTTCGCTTACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGA
TGCAGTGAGCGCCTTCACGCGTTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGG
CCCAGCGACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG
ACCGAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTGCGAAGG
TACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGAG
CTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGG
TTGGGTATGACCAGCCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(194-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

42.049

98.435

0.414
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.153

98.609

0.386
  pilF Neisseria gonorrhoeae MS11

37.193

99.13

0.369