Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KI818_RS05560 Genome accession   NZ_CP075562
Coordinates   1116732..1117706 (-) Length   324 a.a.
NCBI ID   WP_000697217.1    Uniprot ID   A0AAN2D5C1
Organism   Staphylococcus aureus strain Sta2021010     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1111732..1122706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI818_RS05520 (KI818_05490) gcvT 1112233..1113324 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  KI818_RS05525 (KI818_05495) - 1113483..1114007 (-) 525 WP_001015120.1 shikimate kinase -
  KI818_RS05530 (KI818_05500) - 1113997..1114143 (-) 147 WP_001792109.1 hypothetical protein -
  KI818_RS05535 (KI818_05505) comGF 1114240..1114737 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  KI818_RS05540 (KI818_05510) comGE 1114655..1114954 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  KI818_RS05545 (KI818_05515) comGD 1114941..1115387 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  KI818_RS05550 (KI818_05520) comGC 1115365..1115676 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  KI818_RS05555 (KI818_05525) comGB 1115690..1116760 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  KI818_RS05560 (KI818_05530) comGA 1116732..1117706 (-) 975 WP_000697217.1 competence type IV pilus ATPase ComGA Machinery gene
  KI818_RS05565 (KI818_05535) - 1117758..1118381 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  KI818_RS05570 (KI818_05540) - 1118378..1118707 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  KI818_RS05575 (KI818_05545) - 1118707..1119693 (-) 987 WP_000161314.1 ROK family glucokinase -
  KI818_RS05580 (KI818_05550) - 1119690..1119893 (-) 204 WP_000087561.1 YqgQ family protein -
  KI818_RS05585 (KI818_05555) - 1119874..1121337 (-) 1464 WP_001017865.1 rhomboid family intramembrane serine protease -
  KI818_RS05590 (KI818_05560) - 1121349..1121888 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  KI818_RS05595 (KI818_05565) rpmG 1122097..1122246 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36869.90 Da        Isoelectric Point: 8.8516

>NTDB_id=569603 KI818_RS05560 WP_000697217.1 1116732..1117706(-) (comGA) [Staphylococcus aureus strain Sta2021010]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIAPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=569603 KI818_RS05560 WP_000697217.1 1116732..1117706(-) (comGA) [Staphylococcus aureus strain Sta2021010]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGCACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTGGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAATTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997