Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MMZ75_RS14015 Genome accession   NZ_CP093016
Coordinates   3005613..3006077 (+) Length   154 a.a.
NCBI ID   WP_003122079.1    Uniprot ID   P17836
Organism   Pseudomonas aeruginosa strain H15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3000613..3011077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMZ75_RS13990 (MMZ75_13985) yacG 3000783..3000983 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  MMZ75_RS13995 (MMZ75_13990) coaE 3000980..3001591 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  MMZ75_RS14000 (MMZ75_13995) pilD 3001588..3002460 (-) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  MMZ75_RS14005 (MMZ75_14000) pilC 3002461..3003678 (-) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  MMZ75_RS14010 (MMZ75_14005) pilB 3003682..3005382 (-) 1701 WP_241474364.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MMZ75_RS14015 (MMZ75_14010) pilA 3005613..3006077 (+) 465 WP_003122079.1 pilin Machinery gene
  MMZ75_RS14020 (MMZ75_14015) - 3006153..3007538 (+) 1386 WP_003122080.1 O-antigen ligase -
  MMZ75_RS14030 (MMZ75_14025) nadC 3007719..3008567 (-) 849 WP_003122081.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16277.67 Da        Isoelectric Point: 8.9987

>NTDB_id=569480 MMZ75_RS14015 WP_003122079.1 3005613..3006077(+) (pilA) [Pseudomonas aeruginosa strain H15]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSATPKDTQYDIGFTE
STLLDGSGKSQIQVTDNKDGTVELVATLGKSSGSAIKGAVITVSRKNDGVWNCKITKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=569480 MMZ75_RS14015 WP_003122079.1 3005613..3006077(+) (pilA) [Pseudomonas aeruginosa strain H15]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTTGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCAGCGCGACTCCTAAAGATACCCAGTATGACATTGGCTTCACCGAG
TCTACTTTGCTAGATGGTTCTGGTAAGAGTCAGATCCAGGTAACGGACAATAAAGATGGCACCGTTGAGTTGGTCGCTAC
CTTGGGTAAATCTTCTGGTTCCGCCATCAAAGGGGCTGTAATCACTGTTTCGCGTAAAAATGACGGAGTCTGGAACTGCA
AAATCACCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8V7P

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

44.737

98.701

0.442
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.444

99.351

0.442
  pilA Pseudomonas aeruginosa PAK

45.985

88.961

0.409
  pilA2 Legionella pneumophila strain ERS1305867

39.073

98.052

0.383
  pilA Vibrio parahaemolyticus RIMD 2210633

37.255

99.351

0.37
  pilA2 Legionella pneumophila str. Paris

38

97.403

0.37
  pilA Vibrio cholerae strain A1552

36.774

100

0.37
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.774

100

0.37
  pilA Vibrio cholerae C6706

36.774

100

0.37