Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KHS93_RS01745 Genome accession   NZ_CP075547
Coordinates   321578..322015 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SN16-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 316578..327015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHS93_RS19680 - 317157..317318 (-) 162 Protein_297 hypothetical protein -
  KHS93_RS01710 (KHS93_01710) - 317435..318637 (+) 1203 WP_069473143.1 GTP-binding protein -
  KHS93_RS01715 (KHS93_01715) - 318702..319838 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  KHS93_RS01720 (KHS93_01720) - 319853..320287 (+) 435 WP_003156592.1 RDD family protein -
  KHS93_RS01725 (KHS93_01725) - 320359..320679 (+) 321 WP_014304315.1 YckD family protein -
  KHS93_RS01730 (KHS93_01730) - 320783..320899 (+) 117 WP_015388718.1 family 1 glycosylhydrolase -
  KHS93_RS01735 (KHS93_01735) - 320898..321117 (+) 220 Protein_303 family 1 glycosylhydrolase -
  KHS93_RS01740 (KHS93_01740) nin/comJ 321158..321556 (-) 399 WP_043866822.1 competence protein ComJ Regulator
  KHS93_RS01745 (KHS93_01745) nucA/comI 321578..322015 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  KHS93_RS01750 (KHS93_01750) hxlB 322374..322931 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  KHS93_RS01755 (KHS93_01755) hxlA 322928..323563 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  KHS93_RS01760 (KHS93_01760) - 323795..324157 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=569427 KHS93_RS01745 WP_003156588.1 321578..322015(-) (nucA/comI) [Bacillus amyloliquefaciens strain SN16-1]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=569427 KHS93_RS01745 WP_003156588.1 321578..322015(-) (nucA/comI) [Bacillus amyloliquefaciens strain SN16-1]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641