Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KI608_RS20000 Genome accession   NZ_CP075192
Coordinates   4157991..4159460 (-) Length   489 a.a.
NCBI ID   WP_226449349.1    Uniprot ID   -
Organism   Ferribacterium limneticum strain 132     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4152991..4164460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI608_RS19970 (KI608_19910) ttcA 4154014..4154877 (+) 864 WP_226449340.1 tRNA 2-thiocytidine(32) synthetase TtcA -
  KI608_RS19975 (KI608_19915) - 4154955..4155854 (+) 900 WP_226449341.1 adenylate/guanylate cyclase domain-containing protein -
  KI608_RS19980 (KI608_19920) - 4155904..4156158 (-) 255 WP_226449342.1 hypothetical protein -
  KI608_RS19985 (KI608_19925) hprK 4156207..4157142 (-) 936 WP_226449343.1 HPr(Ser) kinase/phosphatase -
  KI608_RS19990 (KI608_19930) ptsN 4157152..4157598 (-) 447 WP_226449345.1 PTS IIA-like nitrogen regulatory protein PtsN -
  KI608_RS19995 (KI608_19935) hpf 4157649..4157975 (-) 327 WP_226449347.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  KI608_RS20000 (KI608_19940) rpoN/rpoN1 4157991..4159460 (-) 1470 WP_226449349.1 RNA polymerase factor sigma-54 Machinery gene
  KI608_RS20005 (KI608_19945) lptB 4159488..4160222 (-) 735 WP_226449351.1 LPS export ABC transporter ATP-binding protein -
  KI608_RS20010 (KI608_19950) recX 4160219..4160650 (-) 432 WP_226449353.1 recombination regulator RecX -
  KI608_RS20015 (KI608_19955) recA 4160643..4161674 (-) 1032 WP_226449355.1 recombinase RecA Machinery gene
  KI608_RS20020 (KI608_19960) - 4161856..4162047 (+) 192 WP_226449357.1 hypothetical protein -
  KI608_RS20025 (KI608_19965) - 4162050..4162706 (+) 657 WP_226449359.1 RluA family pseudouridine synthase -
  KI608_RS20030 (KI608_19970) - 4162778..4164325 (+) 1548 WP_226449361.1 sodium:solute symporter family protein -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 55363.61 Da        Isoelectric Point: 5.2313

>NTDB_id=568614 KI608_RS20000 WP_226449349.1 4157991..4159460(-) (rpoN/rpoN1) [Ferribacterium limneticum strain 132]
MKPALQLKLSQHLALTPQLQQSIKLLQLSTVEMQQEIERYLLENPMLEREDEHAGESFSSAQQFDAPQSSEGEREQKVER
EERDAREEREQDVPSAPSEVDDDRWSSDAGTFTGAGRDEDDDSDSRDIHAANVSLRDHLGWQLGMTQVSERDRSLVRFLI
EALDDDGYLSATLVELWETLPPEYEIEIEELEIALRHIQNFDPIGIGARSLQECLTLQLKVLPESAERTLALSIVDKHLE
LLAARDFAKIRRLTGCDDEALKAAHSLITSLNPRPAAGYAQIEARYITPDVIVKKLKGKWTAYINPDAYPRLRINRLYAE
ILAKQRRGNGNLSTQLQEARWLIKNVQQRFETIHRVTQTIVDRQRQFFEHGEVAMRPLVLREIADILGLHESTVSRVTSQ
KYMATPRGIFELKYFFGSHVATDTGGACSATAIRALIKQLIAAEDGKKPLSDSQLSEILGQQGIVVARRTVAKYRESLNI
PPVNLRKTL

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=568614 KI608_RS20000 WP_226449349.1 4157991..4159460(-) (rpoN/rpoN1) [Ferribacterium limneticum strain 132]
ATGAAGCCTGCCCTCCAGCTAAAACTATCCCAGCATCTGGCGCTGACCCCTCAGCTCCAGCAGTCGATCAAGCTTCTTCA
GTTGTCGACTGTCGAGATGCAGCAGGAAATCGAGCGTTACCTGCTCGAAAACCCAATGCTGGAACGTGAAGACGAGCACG
CTGGCGAGAGTTTCTCATCCGCCCAGCAGTTCGATGCGCCGCAAAGCAGCGAAGGCGAACGCGAGCAGAAGGTTGAGCGG
GAAGAACGGGATGCCCGCGAAGAGCGCGAGCAGGATGTTCCGTCAGCCCCATCCGAAGTGGACGATGATCGCTGGTCTTC
CGATGCCGGTACCTTCACCGGCGCCGGGCGCGACGAGGACGACGATAGCGATTCCCGCGACATCCATGCCGCCAACGTCA
GTCTGCGCGACCATCTAGGCTGGCAGCTGGGCATGACCCAGGTGTCGGAGCGCGACCGCAGTCTGGTGCGCTTCCTGATC
GAAGCGCTCGACGACGATGGTTATCTCTCGGCGACGCTGGTCGAACTGTGGGAAACCCTGCCGCCGGAGTACGAAATTGA
AATCGAAGAGCTGGAAATCGCCTTGCGCCATATCCAGAATTTCGACCCGATCGGCATTGGCGCCCGCAGTCTGCAGGAGT
GTCTGACCCTGCAGCTCAAGGTCTTGCCGGAGAGTGCGGAGCGAACGCTGGCTCTCTCCATTGTCGACAAGCATCTCGAA
CTGCTGGCTGCCCGCGATTTCGCCAAGATTCGTCGCCTGACCGGCTGCGATGACGAGGCCTTGAAGGCCGCGCACAGCCT
GATCACCAGCCTCAACCCGCGGCCGGCGGCCGGCTATGCCCAGATTGAAGCGCGCTACATCACGCCGGACGTGATCGTGA
AAAAGCTGAAGGGCAAATGGACTGCCTACATCAATCCCGACGCCTATCCGCGACTGCGCATCAACCGCCTGTATGCCGAG
ATTCTCGCCAAGCAGCGGCGCGGCAACGGCAACCTGTCCACGCAATTGCAGGAAGCGCGCTGGCTGATCAAGAATGTCCA
GCAGCGCTTTGAAACCATACACCGTGTCACGCAGACCATCGTCGACCGCCAGCGCCAGTTCTTCGAACATGGCGAAGTAG
CCATGCGGCCGCTGGTCTTGCGTGAAATTGCCGATATTCTCGGCCTGCACGAGTCGACCGTATCGCGGGTGACCAGCCAG
AAATACATGGCGACACCACGCGGTATCTTCGAACTGAAATACTTCTTTGGCAGCCATGTCGCCACCGATACCGGTGGTGC
CTGTTCTGCCACGGCCATCCGGGCATTGATCAAGCAGTTGATCGCTGCCGAGGATGGCAAGAAGCCCTTGTCGGATAGCC
AATTATCCGAAATACTAGGCCAACAGGGAATCGTAGTTGCCCGACGGACGGTTGCCAAATACCGCGAGTCGCTCAATATC
CCCCCGGTCAATTTACGCAAGACCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(127-318)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(332-487)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

56.175

100

0.577