Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AVFI_RS01845 Genome accession   NZ_CP092712
Coordinates   403259..404983 (+) Length   574 a.a.
NCBI ID   WP_005417461.1    Uniprot ID   Q5E7Y9
Organism   Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 398259..409983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AVFI_RS01820 (AVFI_01820) pilO 398635..399273 (+) 639 WP_017020160.1 type 4a pilus biogenesis protein PilO -
  AVFI_RS01825 (AVFI_01825) - 399275..399616 (+) 342 WP_012534271.1 hypothetical protein -
  AVFI_RS01830 (AVFI_01830) mshL 399628..401268 (+) 1641 WP_017020159.1 pilus (MSHA type) biogenesis protein MshL -
  AVFI_RS01835 (AVFI_01835) - 401281..402129 (+) 849 WP_012533188.1 ExeA family protein -
  AVFI_RS01840 (AVFI_01840) - 402126..403259 (+) 1134 WP_188863668.1 lipopolysaccharide assembly protein LapB -
  AVFI_RS01845 (AVFI_01845) pilB/pilB1 403259..404983 (+) 1725 WP_005417461.1 GspE/PulE family protein Machinery gene
  AVFI_RS01850 (AVFI_01850) - 404993..406219 (+) 1227 WP_005417463.1 type II secretion system F family protein -
  AVFI_RS01855 (AVFI_01855) - 406222..406677 (+) 456 WP_188863669.1 type IV pilus, mannose-sensitive hemagglutinin protein MshF -
  AVFI_RS01860 (AVFI_01860) - 406764..407327 (+) 564 WP_005417465.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AVFI_RS01865 (AVFI_01865) - 407492..407953 (+) 462 WP_054775771.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AVFI_RS01870 (AVFI_01870) - 408080..408676 (+) 597 WP_054775772.1 type II secretion system protein -
  AVFI_RS01875 (AVFI_01875) - 408685..409254 (+) 570 WP_235630768.1 type II secretion system protein -
  AVFI_RS01880 (AVFI_01880) - 409254..409979 (+) 726 WP_012533580.1 type II secretion system protein J -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 64104.54 Da        Isoelectric Point: 6.0528

>NTDB_id=567928 AVFI_RS01845 WP_005417461.1 403259..404983(+) (pilB/pilB1) [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
MRLKLRKRLGDLLVEENIVTEEQIEQALSTQKKTGRKLGATLIQLGFLSEKQMLTFLSQQLGVPLVDLNRAEIDAAAVQL
LPEVHARRLRALVLSQQGNTIRVAMSDPADLSAQEALFSQLQQYQVEMVIASERQLVDSFDRFYRRTKEIASFAEKLHSE
HQVNDDFNFGISDNDNEEVTVVKLINSLFEDAIQVGASDIHIEPDERVLRLRQRIDGVLHETLLNEVNIASALVLRLKLM
ANLDISEKRLPQDGRFNIKSRGQSVDIRLSTMPVQYGESVVMRLLNQTSGVQELEQSGLPEHLLTRLRKQLRRPHGMILV
TGPTGSGKTTTLYGALSELNQPSKKIITAEDPVEYRLPRVNQVQVNSKIDLSFSKILRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDSVDSALRMMDMGAPGYLVASAVRAVLAQRLVKKVCPECSAPDELDEARKQWVQSRFPNQV
GVTFQRGNGCQSCNLTGYRGRIGVFELLELDQPMMDALRANDAVGFSQKARQNPDYKPLLASAMELAMSGVISLDEVMVL
GEGDNASSHQPILM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=567928 AVFI_RS01845 WP_005417461.1 403259..404983(+) (pilB/pilB1) [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
ATGCGTCTTAAGTTAAGAAAAAGACTTGGTGATCTATTAGTCGAAGAAAACATTGTTACTGAAGAGCAAATCGAACAGGC
TCTTTCGACTCAAAAGAAAACAGGCCGAAAGTTAGGGGCAACATTAATTCAGTTAGGTTTCCTGAGTGAAAAACAAATGT
TGACGTTTTTATCTCAGCAGTTGGGTGTCCCTTTAGTTGATTTGAACCGTGCTGAAATTGATGCGGCAGCCGTGCAGTTG
TTGCCTGAAGTGCATGCTCGTCGCTTACGTGCATTAGTACTTAGCCAGCAAGGCAATACGATTCGAGTTGCCATGAGTGA
TCCTGCTGATCTATCTGCTCAAGAAGCCTTGTTTTCACAGCTTCAGCAATATCAAGTAGAAATGGTCATTGCTTCTGAAA
GACAGCTCGTTGATAGCTTTGATCGTTTCTATCGTAGAACCAAAGAAATCGCTTCATTTGCTGAGAAGTTGCATTCAGAA
CACCAAGTAAACGACGATTTTAATTTTGGTATATCAGATAACGATAATGAAGAAGTAACCGTTGTTAAGCTGATTAACTC
ATTATTTGAAGATGCGATTCAAGTTGGTGCTTCAGATATACATATTGAACCGGATGAAAGAGTCCTACGTTTACGTCAGC
GTATTGATGGGGTATTACACGAAACATTATTAAATGAAGTTAATATCGCATCAGCATTGGTTTTACGTTTAAAACTGATG
GCGAATTTGGATATTTCTGAAAAACGTTTGCCACAAGATGGACGATTTAACATCAAATCTCGTGGTCAGTCTGTGGATAT
TCGTTTATCGACAATGCCAGTTCAATATGGTGAGTCGGTTGTTATGCGTTTATTGAATCAGACATCTGGCGTTCAAGAGC
TAGAACAATCTGGATTACCGGAACATTTACTTACTCGTCTGCGTAAACAACTTCGTCGTCCACATGGCATGATCTTAGTT
ACGGGGCCAACAGGTTCTGGTAAAACGACAACATTGTATGGTGCTTTAAGTGAATTAAATCAACCAAGCAAGAAAATCAT
TACAGCAGAAGATCCGGTTGAATACCGTTTACCTCGTGTTAATCAGGTACAAGTAAATAGCAAAATTGATTTAAGCTTTT
CAAAAATTTTAAGAACGTTTTTACGTCAAGACCCGGATATTATCCTTATTGGTGAAATGCGTGATCAAGAAACTGTGGAA
ATTGGTTTACGTGCCGCTTTAACTGGTCACTTAGTGTTATCTACATTACATACGAATGACTCGGTTGATAGTGCATTACG
TATGATGGATATGGGAGCGCCAGGTTATCTGGTTGCTAGTGCCGTTCGTGCGGTATTAGCGCAACGTCTAGTTAAAAAAG
TGTGTCCAGAGTGCTCCGCTCCTGATGAGTTAGATGAAGCGAGAAAACAGTGGGTACAAAGCCGTTTTCCAAATCAAGTC
GGAGTAACTTTCCAACGTGGAAATGGATGTCAAAGTTGTAATTTGACAGGCTACCGTGGCCGTATTGGTGTGTTTGAATT
ACTAGAGTTGGATCAGCCAATGATGGATGCATTAAGAGCAAATGATGCGGTTGGCTTTTCACAAAAAGCACGTCAAAATC
CTGATTATAAACCACTGCTTGCCTCTGCTATGGAGCTTGCGATGAGTGGTGTGATTAGCTTGGATGAAGTAATGGTATTA
GGTGAAGGAGATAATGCATCTTCTCATCAACCAATATTGATGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(188-453)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5E7Y9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

36.23

100

0.385
  pilF Thermus thermophilus HB27

36.62

98.955

0.362
  pilB Vibrio parahaemolyticus RIMD 2210633

36.814

98.432

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.767

98.084

0.361