Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MJ392_RS18005 Genome accession   NZ_CP092639
Coordinates   3729940..3730380 (+) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain HS     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3724940..3735380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ392_RS17985 (MJ392_17985) aroP 3725938..3727311 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  MJ392_RS17990 (MJ392_17990) ampE 3727354..3728208 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  MJ392_RS17995 (MJ392_17995) ampD 3728205..3728756 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MJ392_RS18000 (MJ392_18000) nadC 3728844..3729737 (+) 894 WP_001135187.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MJ392_RS18005 (MJ392_18005) pilA 3729940..3730380 (+) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  MJ392_RS18010 (MJ392_18010) pilB 3730390..3731775 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  MJ392_RS18015 (MJ392_18015) hofC 3731765..3732967 (+) 1203 WP_000157234.1 protein transport protein HofC -
  MJ392_RS18020 (MJ392_18020) guaC 3733002..3734045 (-) 1044 WP_001217338.1 GMP reductase -
  MJ392_RS18025 (MJ392_18025) - 3734201..3734245 (-) 45 WP_120795372.1 protein YacM -
  MJ392_RS18030 (MJ392_18030) coaE 3734270..3734890 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=567589 MJ392_RS18005 WP_000360902.1 3729940..3730380(+) (pilA) [Escherichia coli strain HS]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=567589 MJ392_RS18005 WP_000360902.1 3729940..3730380(+) (pilA) [Escherichia coli strain HS]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377