Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   MKS87_RS07440 Genome accession   NZ_CP092631
Coordinates   1416281..1418380 (-) Length   699 a.a.
NCBI ID   WP_173614052.1    Uniprot ID   -
Organism   Bacillus subtilis strain YB-15     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 1411281..1423380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKS87_RS07410 (MKS87_07410) spo0E 1411336..1411593 (+) 258 WP_003232480.1 aspartyl-phosphate phosphatase Spo0E -
  MKS87_RS07415 (MKS87_07415) - 1411679..1412110 (+) 432 WP_003232479.1 hypothetical protein -
  MKS87_RS07420 (MKS87_07420) kinD 1412138..1413658 (-) 1521 WP_080477770.1 sporulation kinase KinD -
  MKS87_RS07425 (MKS87_07425) mhqR 1413851..1414288 (+) 438 WP_003232475.1 MarR family transcriptional regulator MhqR -
  MKS87_RS07430 (MKS87_07430) motB 1414328..1415113 (-) 786 WP_003232473.1 flagellar motor protein MotB -
  MKS87_RS07435 (MKS87_07435) motA 1415085..1415897 (-) 813 WP_003238984.1 flagellar motor stator protein MotA -
  MKS87_RS07440 (MKS87_07440) clpE 1416281..1418380 (-) 2100 WP_173614052.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  MKS87_RS07445 (MKS87_07445) - 1418746..1419789 (+) 1044 WP_021479408.1 membrane protein -
  MKS87_RS07450 (MKS87_07450) queC 1420102..1420761 (+) 660 WP_003232465.1 7-cyano-7-deazaguanine synthase QueC -
  MKS87_RS07455 (MKS87_07455) queD 1420754..1421203 (+) 450 WP_003232462.1 6-carboxytetrahydropterin synthase QueD -
  MKS87_RS07460 (MKS87_07460) queE 1421196..1421927 (+) 732 WP_003232460.1 7-carboxy-7-deazaguanine synthase QueE -
  MKS87_RS07465 (MKS87_07465) queF 1421945..1422442 (+) 498 WP_003218613.1 preQ(1) synthase -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77849.99 Da        Isoelectric Point: 5.1809

>NTDB_id=567336 MKS87_RS07440 WP_173614052.1 1416281..1418380(-) (clpE) [Bacillus subtilis strain YB-15]
MRCQHCHQNEATIRLNMQINFVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEQSAAKQSGKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPTIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKTKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQAAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=567336 MKS87_RS07440 WP_173614052.1 1416281..1418380(-) (clpE) [Bacillus subtilis strain YB-15]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTTCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAACAGAGCGCAGCAAAACAAAGCGGAAAAAAAGGGCTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCTAAAGCCGGTCTCATTGATCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGGAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTGGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGTGATGTTCCAAACAAACTGAAAAACAAAGAGCTATATTTGCTTGATGTTGCATCCCTTGTTGCA
AATACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGCTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTCGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTACAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCGTACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCGTGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAACAAAAGCCCTGGAAGAAGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAACTCGAAGCAAAACTTCATGAACGCGTGATTGGACAAGCAGCCGCTGTTCAAAAAGTGGCAAAGGCGGTAAGACG
AAGCCGCGCCGGTTTAAAATCCAAAAACAGGCCAGTCGGCTCCTTCCTCTTCGTCGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAGAAAGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAAGATGGCCGTCTGACAGACAGCCAAGGCAGAACCGTAAGCTTCAAAGACACTGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTAATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCTGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGAGCGAAATAA

Domains

Predicted by InterProScan.

AAA lid domain (AAA_lid_9)

(253-352)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.528

100

0.601
  clpC Lactococcus lactis subsp. cremoris KW2

56.421

100

0.591
  clpE Streptococcus pneumoniae TIGR4

55.062

100

0.568
  clpE Streptococcus pneumoniae Rx1

55.062

100

0.568
  clpE Streptococcus pneumoniae D39

55.062

100

0.568
  clpE Streptococcus pneumoniae R6

55.062

100

0.568
  clpC Bacillus subtilis subsp. subtilis str. 168

53.858

90.844

0.489
  clpC Streptococcus pneumoniae Rx1

45.579

93.848

0.428
  clpC Streptococcus pneumoniae D39

45.579

93.848

0.428
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.528

89.7

0.426
  clpC Streptococcus thermophilus LMD-9

45.274

93.848

0.425
  clpC Streptococcus thermophilus LMG 18311

44.817

93.848

0.421
  clpC Streptococcus pneumoniae TIGR4

46.142

90.844

0.419
  clpC Streptococcus mutans UA159

46.055

88.841

0.409
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

41.749

86.695

0.362