Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AZKH_RS21445 Genome accession   NC_020516
Coordinates   4770452..4771885 (+) Length   477 a.a.
NCBI ID   WP_015437902.1    Uniprot ID   H0PWV3
Organism   Azoarcus sp. KH32C     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4765452..4776885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZKH_RS21415 (AZKH_4319) thiL 4766187..4767146 (+) 960 WP_015437896.1 thiamine-phosphate kinase -
  AZKH_RS21420 (AZKH_4320) - 4767174..4767674 (+) 501 WP_015437897.1 phosphatidylglycerophosphatase A -
  AZKH_RS21425 (AZKH_4321) - 4767659..4768147 (+) 489 WP_015437898.1 CinA family protein -
  AZKH_RS21430 (AZKH_4322) recA 4768224..4769261 (+) 1038 WP_015437899.1 recombinase RecA Machinery gene
  AZKH_RS21435 (AZKH_4323) recX 4769272..4769721 (+) 450 WP_015437900.1 recombination regulator RecX -
  AZKH_RS21440 (AZKH_4324) lptB 4769708..4770430 (+) 723 WP_015437901.1 LPS export ABC transporter ATP-binding protein -
  AZKH_RS21445 (AZKH_4325) rpoN/rpoN1 4770452..4771885 (+) 1434 WP_015437902.1 RNA polymerase factor sigma-54 Machinery gene
  AZKH_RS21450 (AZKH_4326) hpf 4771903..4772226 (+) 324 WP_015437903.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AZKH_RS21455 (AZKH_4327) ptsN 4772361..4772846 (+) 486 WP_041656475.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AZKH_RS21460 (AZKH_4328) hprK 4772815..4773756 (+) 942 WP_015437905.1 HPr(Ser) kinase/phosphatase -
  AZKH_RS21465 (AZKH_4329) ilvA 4773844..4775364 (+) 1521 WP_015437906.1 threonine ammonia-lyase, biosynthetic -
  AZKH_RS21470 (AZKH_4330) - 4775361..4775957 (-) 597 WP_015437907.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53379.08 Da        Isoelectric Point: 4.9481

>NTDB_id=56731 AZKH_RS21445 WP_015437902.1 4770452..4771885(+) (rpoN/rpoN1) [Azoarcus sp. KH32C]
MKPSLQLKISQHLTLTPQLQQSIKLLQLSTLELNQELERFLQDNPMLEREDGDGADFSPGLPNSGSASGEQERSEPTESP
REDAEPTSFDEAGEWSSMSSGGSSRDDDDDTDFQELQAAGTTLRDHLDQQISLSPLSDRDRALVRFLIEALDDDGYLHQT
LEDLLPLLPAELEYELDDLSIALRHVQHLDPAGVGARSPQECLSLQLRAMPGSETRDLAQKIVDQHLDLLAERNFARLKR
LLGCDDDALRRAHALICSLDPHPGSQHSVQETRYVVPDVVVRKLRSRWVVSLNPEAMPRLRINSLYASILQQNRGSGGAL
TSQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDYGDVAMRPLTLREIADQLELHESTVSRVTTQKFMATPRGVFEL
KYFFGSHVATDTGGAASSTAIRALIRQLVDAEDRKKPLSDAKIAELLGQQGIVVARRTIAKYRESLNIPPVSLRKTI

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=56731 AZKH_RS21445 WP_015437902.1 4770452..4771885(+) (rpoN/rpoN1) [Azoarcus sp. KH32C]
ATGAAGCCCTCCCTCCAGCTCAAGATCTCGCAGCACCTCACGCTGACCCCGCAGTTGCAGCAGTCGATCAAGCTGCTGCA
ACTGTCGACCCTTGAACTGAACCAGGAGCTTGAACGCTTCCTGCAGGACAACCCGATGCTCGAGCGCGAAGACGGCGACG
GCGCCGATTTTTCGCCCGGTTTGCCCAACTCGGGCAGCGCCAGCGGCGAGCAGGAACGCAGCGAACCGACCGAAAGCCCG
CGCGAGGACGCCGAACCGACCAGCTTCGACGAAGCCGGCGAATGGTCGAGCATGAGCAGCGGCGGCTCGAGCCGCGACGA
TGACGACGACACCGACTTCCAGGAACTGCAGGCCGCCGGCACCACGCTGCGCGACCACCTCGACCAGCAGATCAGCCTGT
CGCCGCTGTCCGACCGCGACCGCGCGCTGGTGCGCTTCCTCATCGAAGCGCTCGACGACGACGGCTACCTGCACCAGACA
CTCGAAGACCTGCTGCCGCTGCTGCCCGCGGAACTCGAATACGAGCTCGACGACCTGTCGATCGCGCTGCGCCACGTGCA
GCACCTCGACCCCGCCGGCGTCGGCGCCCGCAGTCCGCAGGAGTGCCTGAGCCTGCAATTGCGCGCGATGCCTGGCAGCG
AGACGCGCGATCTCGCGCAGAAGATCGTCGACCAGCATCTCGACCTCCTCGCCGAACGCAACTTCGCACGCCTCAAGCGC
CTGCTGGGCTGCGACGACGACGCACTGCGCCGCGCCCATGCGCTGATCTGCAGCCTCGACCCGCATCCGGGCTCGCAGCA
TTCGGTGCAGGAGACCCGCTACGTGGTCCCGGACGTCGTCGTCCGCAAGCTGCGCTCGCGCTGGGTCGTGAGCCTCAACC
CCGAGGCGATGCCGCGCCTGCGCATCAACTCGCTTTACGCCAGCATCCTGCAACAGAACCGCGGATCGGGCGGCGCGCTG
ACCAGCCAGCTGCAGGAAGCGCGCTGGCTGATCAAGAACGTGCAGCAGCGTTTCGACACGATTCTGCGGGTTTCGCAAGC
GATCGTTGACCAGCAGCGGCAGTTCTTCGATTATGGCGATGTGGCGATGCGCCCTCTGACGCTCAGGGAGATCGCGGACC
AACTGGAGCTGCACGAATCCACGGTCTCGCGGGTCACGACGCAGAAGTTCATGGCCACACCGCGCGGCGTGTTCGAACTG
AAGTATTTTTTCGGCAGCCACGTTGCCACCGATACCGGTGGGGCGGCATCCTCCACGGCGATTCGCGCGCTGATTCGCCA
GCTGGTGGATGCCGAAGACAGGAAAAAACCCCTGTCCGACGCGAAGATTGCCGAATTGTTGGGCCAGCAGGGTATCGTGG
TGGCGCGGAGAACCATCGCGAAGTACCGTGAGTCACTCAATATCCCGCCGGTCAGCCTGCGCAAAACGATCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(113-306)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H0PWV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

57.086

100

0.6

Multiple sequence alignment