Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   WQG_RS09125 Genome accession   NC_020515
Coordinates   1985956..1986528 (-) Length   190 a.a.
NCBI ID   WP_015433244.1    Uniprot ID   A0A426FFN3
Organism   Bibersteinia trehalosi USDA-ARS-USMARC-192     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1980956..1991528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WQG_RS09095 (WQG_18250) - 1981268..1981432 (-) 165 WP_015433237.1 Trm112 family protein -
  WQG_RS09100 (WQG_18260) lpxK 1981425..1982414 (-) 990 WP_015433238.1 tetraacyldisaccharide 4'-kinase -
  WQG_RS09105 (WQG_18280) clpX 1982584..1983837 (-) 1254 WP_015433240.1 ATP-dependent protease ATP-binding subunit ClpX -
  WQG_RS09110 (WQG_18290) clpP 1983837..1984427 (-) 591 WP_025266793.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  WQG_RS09115 (WQG_18300) - 1984535..1984933 (+) 399 WP_015433242.1 SufE family protein -
  WQG_RS09120 (WQG_18310) - 1984915..1985901 (-) 987 WP_015433243.1 AEC family transporter -
  WQG_RS09125 (WQG_18320) pilF 1985956..1986528 (-) 573 WP_015433244.1 tetratricopeptide repeat protein Machinery gene
  WQG_RS09130 (WQG_18330) - 1986627..1987190 (-) 564 WP_015433245.1 anthranilate synthase component II -
  WQG_RS09135 (WQG_18340) rmuC 1987195..1988823 (-) 1629 WP_015433246.1 DNA recombination protein RmuC -
  WQG_RS09140 (WQG_18350) ung 1988892..1989554 (-) 663 WP_015433247.1 uracil-DNA glycosylase -
  WQG_RS09145 (WQG_18360) grcA 1989671..1990054 (+) 384 WP_015433248.1 autonomous glycyl radical cofactor GrcA -
  WQG_RS09150 (WQG_18370) rpsO 1990182..1990451 (-) 270 WP_015433249.1 30S ribosomal protein S15 -

Sequence

Protein


Download         Length: 190 a.a.        Molecular weight: 21357.36 Da        Isoelectric Point: 7.3201

>NTDB_id=56700 WQG_RS09125 WP_015433244.1 1985956..1986528(-) (pilF) [Bibersteinia trehalosi USDA-ARS-USMARC-192]
MFFAKLPQYLSACLLIFSLSGCVNSQTSADFDRDEALIARASLALAYLEQQDFSKAKENIEKALSHNSQHYLAHLVAAYY
YQQLSDVQHAEQSYQAAIRLSHQIKQNSPLPEVLNNYGTFLCQQGKFVQAYTQFEQALTGEKAYLRQGDTLENMVLCAKQ
ANQLEKMAQALARLALLDAERATKLAKIVK

Nucleotide


Download         Length: 573 bp        

>NTDB_id=56700 WQG_RS09125 WP_015433244.1 1985956..1986528(-) (pilF) [Bibersteinia trehalosi USDA-ARS-USMARC-192]
ATGTTTTTCGCAAAATTGCCTCAATATTTAAGCGCTTGCTTGCTGATTTTTTCACTCAGTGGTTGCGTGAACTCACAAAC
GTCAGCTGATTTTGATAGAGATGAAGCACTAATAGCGCGAGCAAGCCTTGCTCTTGCTTATCTTGAACAGCAGGATTTCT
CAAAAGCGAAAGAGAATATAGAGAAAGCCTTGAGCCACAATAGCCAACATTATTTGGCGCACCTTGTTGCAGCTTATTAC
TATCAGCAACTTAGCGATGTTCAGCATGCAGAGCAAAGTTATCAAGCGGCGATTCGTTTAAGTCATCAGATTAAGCAAAA
TTCACCATTGCCTGAGGTTCTCAATAACTATGGGACATTTTTATGCCAACAAGGTAAATTTGTTCAAGCTTATACGCAAT
TTGAGCAAGCACTTACAGGAGAAAAAGCTTATCTACGCCAAGGTGATACATTAGAAAATATGGTATTGTGTGCCAAGCAG
GCTAACCAGTTAGAGAAAATGGCTCAGGCACTAGCGCGCTTAGCGCTATTAGATGCAGAACGGGCTACAAAACTTGCAAA
AATTGTCAAATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat-like domain (TPR_21)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A426FFN3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

54.301

97.895

0.532
  pilF/pilF2 Haemophilus influenzae Rd KW20

43.182

92.632

0.4

Multiple sequence alignment