Detailed information NTG0565940

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KIP77_RS16640
• Genome accession: NZ_CP074710
• Coordinates: 3243695..3244813 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain ATCC 17978
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3238695..3249813

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KIP77_RS16630 (KIP77_16525)	-	3241811..3242503 (-)	693	WP_001108509.1	YggS family pyridoxal phosphate-dependent enzyme	-
KIP77_RS16635 (KIP77_16530)	pilT	3242630..3243667 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
KIP77_RS16640 (KIP77_16535)	pilU	3243695..3244813 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KIP77_RS16645 (KIP77_16540)	fur	3244883..3245320 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
KIP77_RS16650 (KIP77_16545)	-	3245432..3245830 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
KIP77_RS16655 (KIP77_16550)	-	3245885..3246208 (-)	324	WP_000442590.1	RnfH family protein	-
KIP77_RS16660 (KIP77_16555)	-	3246208..3247275 (-)	1068	WP_004748097.1	hypothetical protein	-
KIP77_RS16665 (KIP77_16560)	-	3247428..3247883 (-)	456	WP_000782976.1	bacteriohemerythrin	-
KIP77_RS16670 (KIP77_16565)	-	3248136..3248984 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=565940 KIP77_RS16640 WP_000347034.1 3243695..3244813(+) (pilU) [Acinetobacter baumannii strain ATCC 17978]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=565940 KIP77_RS16640 WP_000347034.1 3243695..3244813(+) (pilU) [Acinetobacter baumannii strain ATCC 17978]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39