Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KIJ28_RS03790 Genome accession   NZ_CP074578
Coordinates   853064..854758 (-) Length   564 a.a.
NCBI ID   WP_024648205.1    Uniprot ID   -
Organism   Pseudomonas syringae strain Susan2139     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 848064..859758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIJ28_RS26305 - 848148..848384 (-) 237 WP_327397062.1 DUF3094 family protein -
  KIJ28_RS03755 (KIJ28_03755) - 848269..848802 (-) 534 WP_213568008.1 MOSC domain-containing protein -
  KIJ28_RS03760 (KIJ28_03760) - 848799..849428 (-) 630 WP_003345130.1 DUF1780 domain-containing protein -
  KIJ28_RS03765 (KIJ28_03765) - 849556..850017 (+) 462 WP_213568009.1 hypothetical protein -
  KIJ28_RS03770 (KIJ28_03770) yacG 850144..850353 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  KIJ28_RS03775 (KIJ28_03775) coaE 850350..850973 (-) 624 WP_024648208.1 dephospho-CoA kinase -
  KIJ28_RS03780 (KIJ28_03780) pilD 850970..851842 (-) 873 WP_213631566.1 A24 family peptidase Machinery gene
  KIJ28_RS03785 (KIJ28_03785) pilC 851844..853061 (-) 1218 WP_024648206.1 type II secretion system F family protein Machinery gene
  KIJ28_RS03790 (KIJ28_03790) pilB 853064..854758 (-) 1695 WP_024648205.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KIJ28_RS03795 (KIJ28_03795) pilA2 854984..855409 (+) 426 WP_003392698.1 pilin Machinery gene
  KIJ28_RS26310 - 855549..855720 (+) 172 Protein_749 DUF433 domain-containing protein -
  KIJ28_RS03800 (KIJ28_03800) - 855768..856313 (-) 546 WP_003345116.1 DUF1523 family protein -
  KIJ28_RS03805 (KIJ28_03805) - 856433..857431 (+) 999 WP_213631567.1 ATP-grasp domain-containing protein -
  KIJ28_RS03810 (KIJ28_03810) - 857486..858625 (+) 1140 WP_096120366.1 trypsin-like peptidase domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62388.65 Da        Isoelectric Point: 6.1188

>NTDB_id=565399 KIJ28_RS03790 WP_024648205.1 853064..854758(-) (pilB) [Pseudomonas syringae strain Susan2139]
MTDAVLTGLAKQLVLAELLTEPVALQAYQQARRDKISLVSYLVQNGLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDNDSGLGNLEDVDLGLD
IEPADGKETSLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRDTLIQEGFPEAK
IGTFKIFGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=565399 KIJ28_RS03790 WP_024648205.1 853064..854758(-) (pilB) [Pseudomonas syringae strain Susan2139]
ATGACTGATGCTGTTCTCACTGGCTTGGCCAAACAATTGGTCTTAGCCGAGCTGCTTACTGAACCTGTTGCGCTGCAAGC
GTATCAACAGGCGCGTCGTGACAAAATTTCGTTAGTGAGCTATTTGGTTCAGAACGGACTAGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCCGATCAGTTTGGCGTCCCCTTCATGGACCTAGCCAGCCTGGACAAAGAAAGCCAGCCTAAAGGCCTG
GTGAGTGAAAAGCTGGTTCGACAACATCACGCGCTGCCGCTGTGGCGACGCGGCAATAAACTGTTTATAGGGATTTCGGA
CCCTACGAATCATCAGGCGGTGACTGATATTCAGTTCAGCACAGGTCTGAACACCGAAGCGATTCTGGTCGAAGATGACA
AGCTGACGACCGCGATAGATCGGTTCTTTGACAATGATAGCGGTCTGGGCAATCTGGAAGATGTCGATCTTGGTCTGGAT
ATCGAACCGGCTGATGGCAAGGAAACTTCCCTCGCTACGCAGAATGACGCAGACGATGCCCCGGTGGTGCGATTCGTCAA
CAAAATGTTGATGGACGCTATTCGGTTAGGATCGTCCGACCTGCACTTTGAGCCTTACGAAAAGATCTTCCGCGTGCGGT
TGCGTACCGACGGCATCTTGCATGAAGTCGCTAGACCACCAATTCATTTGGCGAACCGTATCGCCGCGCGCTTGAAAGTG
ATGGCCAGCCTGGATATCTCGGAACGACGCAAACCGCAGGACGGAAGGGTCAAGTTGCGGGTGTCCAAAACAAAGGCCAT
CGATTTTCGTATGAATACCTTGCCCACACTGTGGGGCGAAAAGATCGTGATGCGGATCCTCGACCCGACCAGCGCTCAGA
TGGGTATCGATGCGCTGGGCTATGAGCCAGAACAAAAGGCTTTGTATCTGGAGGCGCTGAAGCAGCCGCAAGGTATGATT
CTTGTAACCGGCCCGACAGGCTCCGGCAAGACCGTGTCCCTGTATACCGGCCTGAATATCCTTAATACCGTGGATATCAA
CATTTCCACAGCTGAAGACCCGGTAGAGATCAACCTGGAAGGCATCAATCAAGTCAACGTCAACCCGCGCCAGGGACTGG
ACTTTTCCCAAGCCTTGCGCGCATTTTTGCGTCAAGATCCCGACGTGATCATGGTCGGCGAGATACGAGACCTGGAAACT
GCCGAAATCGCCATCAAAGCTTCGCAGACTGGCCATATGGTGCTTTCCACACTGCACACCAACAGCGCGGCGGAAACATT
GACTCGCCTGCACCATATGGGCGTTGCAGCATTCAATATTGCAACAGCGATCAACCTGATTATTGCGCAACGACTTGCGC
GGAAGCTGTGTAGCCATTGCAAGAAAGAACTGGATATCCCTCGCGACACACTGATTCAGGAAGGTTTTCCAGAAGCTAAA
ATCGGCACCTTCAAGATCTTTGGACCGGTGGGCTGTGAGCACTGCAATGGGGGCTACAGAGGCAGGGTCGGTATTTACGA
AGTGGTCAAAAAAACACCGGAGCTGGAGCGCATCATCATGGAAGAAGGCAACTCACTGGAGATCTCCAGACAGATGCGCA
AGGACGGCTTCAATGACCTGCGTACCTCCGGACTGTCAAAGGCCATGCAGGGCATAACCAGTCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.848

100

0.582
  pilB Acinetobacter baylyi ADP1

56.714

100

0.569
  pilB Legionella pneumophila strain ERS1305867

55.044

100

0.551
  pilB Vibrio cholerae strain A1552

50.534

99.645

0.504
  pilB Vibrio campbellii strain DS40M4

49.647

100

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

51.371

96.986

0.498
  pilF Neisseria gonorrhoeae MS11

48.043

99.645

0.479
  pilF Thermus thermophilus HB27

39.332

95.567

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.783

93.262

0.362