Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   KIH94_RS12605 Genome accession   NZ_CP074571
Coordinates   2461133..2461750 (-) Length   205 a.a.
NCBI ID   WP_015384132.1    Uniprot ID   -
Organism   Bacillus subtilis strain BYS2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2456133..2466750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIH94_RS12575 (KIH94_12575) rpsT 2456435..2456701 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  KIH94_RS12580 (KIH94_12580) holA 2456716..2457759 (-) 1044 WP_015384130.1 DNA polymerase III subunit delta -
  KIH94_RS12585 (KIH94_12585) - 2457799..2457948 (-) 150 WP_003229985.1 hypothetical protein -
  KIH94_RS12590 (KIH94_12590) yqzM 2457989..2458123 (+) 135 WP_003229983.1 YqzM family protein -
  KIH94_RS12595 (KIH94_12595) comEC 2458163..2460493 (-) 2331 WP_041517945.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  KIH94_RS12600 (KIH94_12600) comEB 2460497..2461066 (-) 570 WP_003229978.1 ComE operon protein 2 -
  KIH94_RS12605 (KIH94_12605) comEA 2461133..2461750 (-) 618 WP_015384132.1 competence protein ComEA Machinery gene
  KIH94_RS12610 (KIH94_12610) comER 2461834..2462655 (+) 822 WP_004398597.1 late competence protein ComER -
  KIH94_RS12615 (KIH94_12615) yqeM 2462721..2463464 (-) 744 WP_015384134.1 class I SAM-dependent methyltransferase -
  KIH94_RS12620 (KIH94_12620) rsfS 2463461..2463817 (-) 357 WP_014480315.1 ribosome silencing factor -
  KIH94_RS12625 (KIH94_12625) yqeK 2463835..2464395 (-) 561 WP_015483448.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  KIH94_RS12630 (KIH94_12630) nadD 2464385..2464954 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  KIH94_RS12635 (KIH94_12635) yhbY 2464966..2465256 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  KIH94_RS12640 (KIH94_12640) aroE 2465250..2466092 (-) 843 WP_015384136.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21744.44 Da        Isoelectric Point: 4.7155

>NTDB_id=565318 KIH94_RS12605 WP_015384132.1 2461133..2461750(-) (comEA) [Bacillus subtilis strain BYS2]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKDLVKQAIPTETENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=565318 KIH94_RS12605 WP_015384132.1 2461133..2461750(-) (comEA) [Bacillus subtilis strain BYS2]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGACCTGGTGAAGCAAGCTATACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCCTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus N315

33.784

100

0.366