Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KIH94_RS01950 Genome accession   NZ_CP074571
Coordinates   364313..364762 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain BYS2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 359313..369762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIH94_RS01915 (KIH94_01915) yckA 359458..360138 (-) 681 WP_015382730.1 amino acid ABC transporter permease -
  KIH94_RS01920 (KIH94_01920) yckB 360148..361011 (-) 864 WP_015482776.1 transporter substrate-binding domain-containing protein -
  KIH94_RS01925 (KIH94_01925) - 361173..361370 (+) 198 WP_144052033.1 hypothetical protein -
  KIH94_RS01930 (KIH94_01930) yckC 361420..361851 (+) 432 WP_046340202.1 RDD family protein -
  KIH94_RS01935 (KIH94_01935) yckD 361933..362265 (+) 333 WP_015252849.1 YckD family protein -
  KIH94_RS01940 (KIH94_01940) bglC 362418..363851 (+) 1434 WP_015382735.1 6-phospho-beta-glucosidase -
  KIH94_RS01945 (KIH94_01945) nin/comJ 363888..364286 (-) 399 WP_015382736.1 DNA-entry nuclease inhibitor Regulator
  KIH94_RS01950 (KIH94_01950) nucA/comI 364313..364762 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  KIH94_RS01955 (KIH94_01955) tlpC 364928..366649 (-) 1722 WP_041517645.1 methyl-accepting chemotaxis protein TlpC -
  KIH94_RS01960 (KIH94_01960) hxlB 366760..367317 (-) 558 WP_015482781.1 6-phospho-3-hexuloisomerase -
  KIH94_RS01965 (KIH94_01965) hxlA 367323..367954 (-) 632 Protein_343 3-hexulose-6-phosphate synthase -
  KIH94_RS01970 (KIH94_01970) hxlR 368186..368548 (+) 363 WP_015482783.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=565275 KIH94_RS01950 WP_014475770.1 364313..364762(-) (nucA/comI) [Bacillus subtilis strain BYS2]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=565275 KIH94_RS01950 WP_014475770.1 364313..364762(-) (nucA/comI) [Bacillus subtilis strain BYS2]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGATATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987