Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MF614_RS19310 Genome accession   NZ_CP092252
Coordinates   3983017..3983457 (+) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain ISHS7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3978017..3988457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MF614_RS19290 (MF614_19290) aroP 3979015..3980388 (+) 1374 WP_000969914.1 aromatic amino acid transporter AroP -
  MF614_RS19295 (MF614_19295) ampE 3980431..3981285 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  MF614_RS19300 (MF614_19300) ampD 3981282..3981833 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MF614_RS19305 (MF614_19305) nadC 3981921..3982814 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MF614_RS19310 (MF614_19310) pilA 3983017..3983457 (+) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  MF614_RS19315 (MF614_19315) pilB 3983467..3984852 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  MF614_RS19320 (MF614_19320) hofC 3984842..3986044 (+) 1203 WP_000157234.1 protein transport protein HofC -
  MF614_RS19325 (MF614_19325) guaC 3986079..3987122 (-) 1044 WP_001217338.1 GMP reductase -
  MF614_RS19330 (MF614_19330) - 3987278..3987322 (-) 45 WP_120795372.1 protein YacM -
  MF614_RS19335 (MF614_19335) coaE 3987347..3987967 (+) 621 WP_094320677.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=564918 MF614_RS19310 WP_000360902.1 3983017..3983457(+) (pilA) [Escherichia coli strain ISHS7]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=564918 MF614_RS19310 WP_000360902.1 3983017..3983457(+) (pilA) [Escherichia coli strain ISHS7]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377