Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KGI96_RS01205 Genome accession   NZ_CP074346
Coordinates   255170..255739 (+) Length   189 a.a.
NCBI ID   WP_211599132.1    Uniprot ID   -
Organism   Pasteurella atlantica strain NVI-9100     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 250170..260739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KGI96_RS01185 (KGI96_01185) - 250914..252239 (-) 1326 WP_211599102.1 patatin-like phospholipase family protein -
  KGI96_RS01190 (KGI96_01190) yfaE 252339..252587 (-) 249 WP_211599103.1 class I ribonucleotide reductase maintenance protein YfaE -
  KGI96_RS01195 (KGI96_01195) nrdB 252587..253717 (-) 1131 WP_211599104.1 class Ia ribonucleoside-diphosphate reductase subunit beta -
  KGI96_RS01200 (KGI96_01200) - 254053..255156 (+) 1104 WP_229577254.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  KGI96_RS01205 (KGI96_01205) pilF 255170..255739 (+) 570 WP_211599132.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  KGI96_RS01210 (KGI96_01210) sohB 255832..256914 (-) 1083 WP_211599106.1 protease SohB -
  KGI96_RS01215 (KGI96_01215) folC 256933..258234 (-) 1302 WP_211599107.1 bifunctional tetrahydrofolate synthase/dihydrofolate synthase -
  KGI96_RS01220 (KGI96_01220) accD 258234..259142 (-) 909 WP_211599108.1 acetyl-CoA carboxylase, carboxyltransferase subunit beta -
  KGI96_RS01225 (KGI96_01225) - 259435..259989 (+) 555 WP_211599109.1 YqiA/YcfP family alpha/beta fold hydrolase -

Sequence

Protein


Download         Length: 189 a.a.        Molecular weight: 21611.58 Da        Isoelectric Point: 9.1873

>NTDB_id=563992 KGI96_RS01205 WP_211599132.1 255170..255739(+) (pilF) [Pasteurella atlantica strain NVI-9100]
MKIANFYSNLTACIIALTLTGCVSTSTEHSINKTEAMQARLNLALAYLKENNFPKAKLNIDKALEHNNQHYLPYSVLAYY
YQQTGKVSDASQTYQQAVTLSQKFTKDHQPRPDILNNYGTFLCKQAQFKQAYNLFEQALTSKQSYYHQADTLENIILCAK
GEKNHTQMNNALIQLRKIDKERAEKISSM

Nucleotide


Download         Length: 570 bp        

>NTDB_id=563992 KGI96_RS01205 WP_211599132.1 255170..255739(+) (pilF) [Pasteurella atlantica strain NVI-9100]
ATGAAAATTGCAAATTTTTATTCAAATCTTACCGCTTGTATAATTGCTTTGACTTTGACAGGTTGTGTTTCTACATCAAC
AGAACACTCAATAAACAAAACAGAGGCAATGCAAGCAAGACTCAACCTTGCACTTGCTTATTTAAAAGAAAATAATTTTC
CAAAAGCAAAATTAAATATCGACAAAGCACTGGAACATAACAATCAGCACTATTTACCTTATTCAGTACTTGCTTATTAC
TATCAACAGACAGGTAAAGTTTCAGATGCATCACAAACCTATCAACAAGCGGTTACATTAAGTCAAAAATTTACAAAAGA
TCACCAACCTCGTCCTGATATATTAAATAATTATGGAACTTTTTTATGTAAACAAGCCCAATTTAAACAAGCTTATAATT
TATTTGAACAAGCATTAACAAGTAAACAATCTTATTATCACCAAGCTGATACGCTAGAAAATATTATACTTTGTGCTAAA
GGTGAGAAAAACCATACTCAAATGAACAACGCATTAATTCAGTTAAGAAAAATAGATAAAGAACGAGCAGAAAAAATTAG
TTCAATGTGA

Domains

Predicted by InterproScan.

Coatomer epsilon subunit (Coatomer_E)

(31-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

58.602

98.413

0.577
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.111

95.238

0.439