Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   H175_RS21440 Genome accession   NC_020376
Coordinates   4237852..4238895 (-) Length   347 a.a.
NCBI ID   WP_001013177.1    Uniprot ID   -
Organism   Bacillus thuringiensis serovar thuringiensis str. IS5056     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4232852..4243895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H175_RS21395 (H175_ch4317) - 4233286..4233486 (-) 201 WP_000106083.1 YqzE family protein -
  H175_RS21400 (H175_ch4318) aroK 4233525..4234022 (-) 498 WP_000836649.1 shikimate kinase AroK -
  H175_RS21405 (H175_ch4319) - 4234138..4234788 (-) 651 WP_000183853.1 2OG-Fe(II) oxygenase -
  H175_RS21410 (H175_ch4320) comGG 4234962..4235333 (-) 372 WP_001214374.1 competence type IV pilus minor pilin ComGG -
  H175_RS21415 (H175_ch4321) comGF 4235330..4235800 (-) 471 WP_000923097.1 competence type IV pilus minor pilin ComGF -
  H175_RS21420 (H175_ch4322) - 4235770..4236072 (-) 303 WP_000829462.1 hypothetical protein -
  H175_RS21425 (H175_ch4323) comGD 4236065..4236520 (-) 456 WP_002094140.1 competence type IV pilus minor pilin ComGD -
  H175_RS21430 (H175_ch4324) comGC 4236517..4236816 (-) 300 WP_001243321.1 competence type IV pilus major pilin ComGC -
  H175_RS21435 (H175_ch4325) comGB 4236828..4237859 (-) 1032 WP_000475180.1 competence type IV pilus assembly protein ComGB -
  H175_RS21440 (H175_ch4326) comGA 4237852..4238895 (-) 1044 WP_001013177.1 competence type IV pilus ATPase ComGA Machinery gene
  H175_RS21445 (H175_ch4327) - 4239101..4239796 (+) 696 WP_000434124.1 helix-turn-helix domain-containing protein -
  H175_RS21450 (H175_ch4328) - 4239923..4240165 (+) 243 WP_000375619.1 DUF2626 domain-containing protein -
  H175_RS21455 (H175_ch4329) - 4240273..4241667 (+) 1395 WP_001094344.1 L-cystine transporter -
  H175_RS37830 - 4241747..4242139 (-) 393 Protein_4281 hypothetical protein -
  H175_RS21465 (H175_ch4332) - 4242227..4242442 (-) 216 WP_001008326.1 DUF3912 family protein -
  H175_RS21470 (H175_ch4333) - 4242696..4243007 (+) 312 WP_001093240.1 hypothetical protein -
  H175_RS21475 (H175_ch4334) - 4243042..4243530 (-) 489 WP_000764263.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39177.60 Da        Isoelectric Point: 8.8944

>NTDB_id=56302 H175_RS21440 WP_001013177.1 4237852..4238895(-) (comGA) [Bacillus thuringiensis serovar thuringiensis str. IS5056]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRHCIKKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEDDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=56302 H175_RS21440 WP_001013177.1 4237852..4238895(-) (comGA) [Bacillus thuringiensis serovar thuringiensis str. IS5056]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACATTGTATTAAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTGCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTCATGACAACGTTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATAAGAAAAGGATATGCTTTAGGTTTTTTAGAAGATGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.196

100

0.562
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369

Multiple sequence alignment