Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KFV12_RS08175 Genome accession   NZ_CP073819
Coordinates   1544070..1545038 (-) Length   322 a.a.
NCBI ID   WP_254256546.1    Uniprot ID   -
Organism   Macrococcus epidermidis strain Epi3002-OL     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1539070..1550038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFV12_RS08145 (KFV12_08180) gcvT 1539719..1540804 (-) 1086 WP_111715536.1 glycine cleavage system aminomethyltransferase GcvT -
  KFV12_RS08150 (KFV12_08185) - 1540942..1541448 (-) 507 WP_111715158.1 shikimate kinase -
  KFV12_RS08155 (KFV12_08190) - 1541631..1542077 (-) 447 WP_239242759.1 competence type IV pilus minor pilin ComGF -
  KFV12_RS08160 (KFV12_08195) - 1542257..1542727 (-) 471 WP_170122460.1 hypothetical protein -
  KFV12_RS08165 (KFV12_08200) comGC 1542678..1543034 (-) 357 WP_255724760.1 competence type IV pilus major pilin ComGC Machinery gene
  KFV12_RS08170 (KFV12_08205) comGB 1543046..1544083 (-) 1038 WP_170122461.1 competence type IV pilus assembly protein ComGB -
  KFV12_RS08175 (KFV12_08210) comGA 1544070..1545038 (-) 969 WP_254256546.1 competence type IV pilus ATPase ComGA Machinery gene
  KFV12_RS08180 (KFV12_08215) - 1545248..1545496 (+) 249 WP_099578437.1 DUF2626 domain-containing protein -
  KFV12_RS08185 (KFV12_08220) - 1545524..1546153 (-) 630 WP_254256547.1 MBL fold metallo-hydrolase -
  KFV12_RS08190 (KFV12_08225) - 1546246..1546485 (+) 240 WP_096076359.1 DUF2759 domain-containing protein -
  KFV12_RS08195 (KFV12_08230) - 1546522..1546833 (-) 312 WP_111715170.1 MTH1187 family thiamine-binding protein -
  KFV12_RS08200 (KFV12_08235) - 1546833..1547804 (-) 972 WP_111715172.1 ROK family glucokinase -
  KFV12_RS08205 (KFV12_08240) - 1547801..1547992 (-) 192 WP_111715173.1 YqgQ family protein -
  KFV12_RS08210 (KFV12_08245) - 1547997..1549421 (-) 1425 WP_111715175.1 rhomboid family intramembrane serine protease -
  KFV12_RS08215 (KFV12_08250) - 1549418..1549984 (-) 567 WP_111715177.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 322 a.a.        Molecular weight: 36772.56 Da        Isoelectric Point: 6.7709

>NTDB_id=560964 KFV12_RS08175 WP_254256546.1 1544070..1545038(-) (comGA) [Macrococcus epidermidis strain Epi3002-OL]
MEKLLHTILNDAITKKTSDVHFIPEADNVMMKFRIGGNMYAYNQLNTEQFEKLLSYVKFITHLDVSEKNKAQSGILTYEK
DEILYNIRASTLPHTIGHEACVMRIIPALFSQNISIEQELLSLMHKGSGLILISGPTGCGKSTLMYQMLNYAALNLNKQI
ISIEDPVEQRIQGMIQINVNKKANITYQNAIKAILRCDPDIIMIGEIRDAETAKQVINASLSGHLVLSTLHANTCFGAIH
RLMEMGVTKVELEQCLEIIINQRLVNTTTNRTRIFEQLIKGDVRNYLNDRKVQHYHPLSEQLQVMHDEEKITQLEYERYA
LE

Nucleotide


Download         Length: 969 bp        

>NTDB_id=560964 KFV12_RS08175 WP_254256546.1 1544070..1545038(-) (comGA) [Macrococcus epidermidis strain Epi3002-OL]
ATGGAAAAACTATTACATACAATATTGAATGATGCAATCACTAAAAAAACTTCAGACGTACACTTTATACCCGAAGCTGA
TAATGTCATGATGAAATTCAGAATTGGAGGTAATATGTATGCTTATAATCAGTTAAATACTGAACAATTCGAAAAATTAT
TATCATATGTAAAGTTTATAACACATCTTGATGTTTCTGAAAAAAATAAAGCACAGAGCGGTATATTGACCTATGAGAAG
GATGAAATACTTTACAATATCAGGGCATCGACATTACCGCATACGATTGGACATGAAGCTTGTGTGATGCGAATAATTCC
AGCATTATTTAGTCAAAATATTTCAATCGAACAGGAATTATTATCTTTAATGCATAAAGGAAGCGGATTAATTTTGATTA
GTGGTCCTACGGGATGCGGAAAAAGTACATTGATGTACCAAATGTTAAATTATGCAGCGCTAAATTTAAATAAACAGATT
ATTTCGATTGAAGATCCCGTTGAACAGAGAATTCAAGGTATGATTCAAATCAATGTAAATAAAAAAGCTAATATAACATA
TCAGAATGCAATAAAGGCCATTCTTCGCTGTGATCCGGATATTATTATGATAGGAGAAATTCGTGATGCTGAAACTGCAA
AACAAGTCATCAATGCAAGTTTAAGTGGTCATCTTGTGCTGTCGACACTGCATGCAAATACATGTTTTGGTGCTATTCAT
CGGTTGATGGAAATGGGGGTAACCAAAGTTGAACTTGAGCAGTGTCTGGAAATAATCATTAATCAGCGATTAGTAAATAC
TACTACAAATCGAACACGTATTTTTGAGCAGTTGATAAAAGGCGATGTACGTAATTATTTAAATGATAGAAAAGTTCAGC
ATTATCATCCTTTATCAGAACAATTGCAAGTTATGCACGACGAAGAAAAGATTACGCAGCTGGAGTATGAACGTTATGCT
TTGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

51.125

96.584

0.494
  comGA Staphylococcus aureus N315

51.125

96.584

0.494