Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KFV10_RS06810 Genome accession   NZ_CP073808
Coordinates   1325833..1326804 (-) Length   323 a.a.
NCBI ID   WP_254253383.1    Uniprot ID   -
Organism   Macrococcoides canis strain IMD0709     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1320833..1331804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFV10_RS06775 (KFV10_06765) gcvT 1321528..1322613 (-) 1086 WP_254254448.1 glycine cleavage system aminomethyltransferase GcvT -
  KFV10_RS06780 (KFV10_06770) - 1322751..1323257 (-) 507 WP_086042649.1 shikimate kinase -
  KFV10_RS06785 (KFV10_06775) - 1323418..1323870 (-) 453 WP_254253379.1 competence type IV pilus minor pilin ComGF -
  KFV10_RS06790 (KFV10_06780) - 1323815..1324057 (-) 243 WP_133418987.1 hypothetical protein -
  KFV10_RS06795 (KFV10_06785) comGD 1324044..1324487 (-) 444 WP_254253380.1 competence type IV pilus minor pilin ComGD -
  KFV10_RS06800 (KFV10_06790) comGC 1324462..1324794 (-) 333 WP_254253381.1 competence type IV pilus major pilin ComGC -
  KFV10_RS06805 (KFV10_06795) comGB 1324809..1325858 (-) 1050 WP_254253382.1 competence type IV pilus assembly protein ComGB -
  KFV10_RS06810 (KFV10_06800) comGA 1325833..1326804 (-) 972 WP_254253383.1 competence type IV pilus ATPase ComGA Machinery gene
  KFV10_RS06815 (KFV10_06805) - 1327016..1327264 (+) 249 WP_086042733.1 DUF2626 domain-containing protein -
  KFV10_RS06820 (KFV10_06810) - 1327292..1327912 (-) 621 WP_254253384.1 MBL fold metallo-hydrolase -
  KFV10_RS06825 (KFV10_06815) - 1328002..1328241 (+) 240 WP_174804788.1 DUF2759 domain-containing protein -
  KFV10_RS06830 (KFV10_06820) - 1328268..1328579 (-) 312 WP_254253385.1 MTH1187 family thiamine-binding protein -
  KFV10_RS06835 (KFV10_06825) - 1328579..1329547 (-) 969 WP_254253386.1 ROK family glucokinase -
  KFV10_RS06840 (KFV10_06830) - 1329544..1329735 (-) 192 WP_086042738.1 YqgQ family protein -
  KFV10_RS06845 (KFV10_06835) - 1329740..1331188 (-) 1449 WP_254253387.1 rhomboid family protein -
  KFV10_RS06850 (KFV10_06840) - 1331164..1331730 (-) 567 WP_086042740.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 37271.80 Da        Isoelectric Point: 6.7236

>NTDB_id=560900 KFV10_RS06810 WP_254253383.1 1325833..1326804(-) (comGA) [Macrococcoides canis strain IMD0709]
MEQLFNEIIEEAIIQGASDIHFIPNDKSISIKFRIHGDIEDYREIDDMLFRKLLSYIKFTAHLDVSEKNKAQSGIIHFNL
KDLRYNIRASTLPRALGDEACVLRIIRQNFIDEYQTNDKILFNQIKKSSGIIIISGPTGSGKSTLMYQLVHYARNTLKRQ
IISIEDPVEQHIDGIIQVNVNEKAEITYHTAIKAILRCDPDIIMLGEIRDSVVAQQVINAGLSGHLVLTTLHANDCIGAL
FRLKEMGINTVDLYQSINLIINQRLVRKKDGKGRLLTYEFLTKKEIEKYLQGDHINYRTLTDQFKELYETNQISHYEFEK
FDQ

Nucleotide


Download         Length: 972 bp        

>NTDB_id=560900 KFV10_RS06810 WP_254253383.1 1325833..1326804(-) (comGA) [Macrococcoides canis strain IMD0709]
ATGGAACAATTATTCAATGAAATAATAGAGGAAGCAATTATACAAGGTGCATCAGATATTCACTTCATACCAAACGATAA
AAGCATATCTATCAAATTTAGGATACATGGTGATATTGAAGACTATAGAGAAATCGACGACATGTTATTCAGGAAATTAC
TTTCTTATATTAAATTTACGGCACATCTTGATGTATCAGAAAAGAATAAGGCACAGAGTGGAATCATCCATTTTAATCTG
AAAGACTTAAGATATAATATTCGCGCATCTACTTTACCTCGAGCATTAGGCGATGAAGCATGTGTACTGAGAATCATCAG
ACAAAATTTTATAGATGAATATCAGACGAATGACAAGATATTGTTCAATCAAATAAAAAAATCAAGCGGTATTATCATCA
TAAGTGGACCAACTGGAAGCGGGAAAAGCACATTGATGTATCAGCTTGTACATTATGCGAGGAACACATTGAAGAGACAA
ATAATATCAATAGAAGATCCTGTTGAACAGCATATTGATGGAATTATACAAGTTAATGTTAACGAAAAAGCAGAGATAAC
ATATCATACCGCGATAAAGGCAATCCTTAGATGTGATCCGGATATCATTATGCTAGGTGAAATCAGAGATTCAGTCGTAG
CACAGCAAGTGATCAATGCAGGACTAAGCGGTCATCTCGTTTTAACGACATTACATGCGAATGATTGCATCGGTGCATTA
TTTCGGTTAAAAGAAATGGGTATTAATACTGTTGATCTATATCAAAGTATCAATCTGATTATTAATCAAAGGCTTGTAAG
AAAAAAAGATGGGAAGGGGCGTCTATTGACCTATGAATTTTTGACAAAAAAGGAAATTGAAAAATATTTACAAGGTGATC
ATATTAACTATAGAACGTTGACCGATCAATTTAAGGAGCTCTATGAAACAAATCAGATTTCACACTATGAATTCGAAAAA
TTTGATCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

49.085

100

0.498
  comGA Staphylococcus aureus N315

49.085

100

0.498
  pilB Glaesserella parasuis strain SC1401

35.26

100

0.378
  comGA Bacillus subtilis subsp. subtilis str. 168

37.423

100

0.378
  pilB Vibrio parahaemolyticus RIMD 2210633

34.925

100

0.362