Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KFV08_RS06965 Genome accession   NZ_CP073805
Coordinates   1361565..1362536 (-) Length   323 a.a.
NCBI ID   WP_252455213.1    Uniprot ID   -
Organism   Macrococcoides canis strain Epi3241-OR     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1356565..1367536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFV08_RS06930 (KFV08_06925) gcvT 1357260..1358345 (-) 1086 WP_211554736.1 glycine cleavage system aminomethyltransferase GcvT -
  KFV08_RS06935 (KFV08_06930) - 1358483..1358989 (-) 507 WP_252455208.1 shikimate kinase -
  KFV08_RS06940 (KFV08_06935) - 1359150..1359602 (-) 453 WP_210151817.1 competence type IV pilus minor pilin ComGF -
  KFV08_RS06945 (KFV08_06940) - 1359547..1359789 (-) 243 WP_252455209.1 hypothetical protein -
  KFV08_RS06950 (KFV08_06945) comGD 1359776..1360201 (-) 426 WP_254250668.1 competence type IV pilus minor pilin ComGD -
  KFV08_RS06955 (KFV08_06950) comGC 1360194..1360526 (-) 333 WP_174804787.1 competence type IV pilus major pilin ComGC -
  KFV08_RS06960 (KFV08_06955) comGB 1360541..1361590 (-) 1050 WP_254250669.1 competence type IV pilus assembly protein ComGB -
  KFV08_RS06965 (KFV08_06960) comGA 1361565..1362536 (-) 972 WP_252455213.1 competence type IV pilus ATPase ComGA Machinery gene
  KFV08_RS06970 (KFV08_06965) - 1362749..1362997 (+) 249 WP_086042733.1 DUF2626 domain-containing protein -
  KFV08_RS06975 (KFV08_06970) - 1363025..1363645 (-) 621 WP_252455214.1 MBL fold metallo-hydrolase -
  KFV08_RS06980 (KFV08_06975) - 1363735..1363974 (+) 240 WP_174804788.1 DUF2759 domain-containing protein -
  KFV08_RS06985 (KFV08_06980) - 1364000..1364311 (-) 312 WP_086042736.1 MTH1187 family thiamine-binding protein -
  KFV08_RS06990 (KFV08_06985) - 1364311..1365279 (-) 969 WP_138071475.1 ROK family glucokinase -
  KFV08_RS06995 (KFV08_06990) - 1365276..1365467 (-) 192 WP_086042738.1 YqgQ family protein -
  KFV08_RS07000 (KFV08_06995) - 1365472..1366920 (-) 1449 WP_254250670.1 rhomboid family protein -
  KFV08_RS07005 (KFV08_07000) - 1366896..1367450 (-) 555 WP_158290713.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 37245.91 Da        Isoelectric Point: 6.8834

>NTDB_id=560867 KFV08_RS06965 WP_252455213.1 1361565..1362536(-) (comGA) [Macrococcoides canis strain Epi3241-OR]
MEKLFNEIIEVAIIQSASDIHFIPNDKSISIKFRIHGDIEDYREIDDMLFRKLLSYIKFTAHLDVSEKNKAQSGIIQFNL
KDLRYNIRASTLPRSLGDEACVLRIIRQNFIDEYQTNDKILYNQIKKSSGIIIISGPTGSGKSTLMYQLVHYARNTLKRQ
IISIEDPVEQHIEGIIQVNVNEKAEITYHTAIKAILRCDPDIIMLGEIRDSVVAHQVINAGLSGHLVLTTLHANDCIGAL
FRLKEMGINAVDLYQSINLIINQRLVRKKDGMGRLLAYEFLTKKDIEKYLQGEHINYRTLTDQFKELYETNQISHYEFEK
FDL

Nucleotide


Download         Length: 972 bp        

>NTDB_id=560867 KFV08_RS06965 WP_252455213.1 1361565..1362536(-) (comGA) [Macrococcoides canis strain Epi3241-OR]
ATGGAAAAGTTATTCAATGAAATAATAGAGGTAGCAATTATACAAAGTGCATCAGATATTCACTTCATACCAAACGATAA
AAGCATATCTATCAAATTTAGGATACATGGTGATATTGAAGACTATAGAGAAATCGACGACATGTTATTCAGGAAATTAC
TTTCTTATATTAAATTTACAGCACATCTTGATGTATCAGAAAAGAATAAGGCACAGAGTGGAATCATCCAATTTAATCTG
AAAGACTTAAGATATAATATTCGCGCATCTACTTTACCTCGATCATTAGGCGATGAAGCATGTGTACTGAGAATCATCAG
ACAAAATTTTATAGATGAATATCAGACGAATGACAAGATATTGTACAATCAAATAAAAAAATCAAGTGGTATTATCATCA
TAAGTGGACCAACTGGAAGCGGGAAAAGCACATTGATGTATCAGCTTGTACATTATGCGAGGAACACATTGAAGAGACAA
ATAATATCAATAGAAGATCCTGTTGAACAGCATATTGAAGGAATTATACAGGTTAATGTTAACGAAAAAGCAGAGATAAC
GTATCATACTGCGATAAAGGCAATCCTTAGATGTGACCCGGATATCATTATGCTAGGTGAAATCAGAGATTCAGTCGTAG
CACATCAAGTGATCAATGCAGGACTAAGCGGTCATCTTGTTTTAACGACATTACATGCGAATGATTGCATCGGTGCATTA
TTTCGGTTAAAAGAAATGGGTATTAATGCTGTTGATCTATATCAAAGTATCAATCTGATTATTAATCAAAGGCTTGTAAG
AAAAAAAGATGGGATGGGGCGTCTATTGGCCTATGAATTTTTAACAAAAAAGGATATTGAAAAATATTTACAAGGTGAAC
ATATTAACTATAGAACGTTGACCGATCAATTTAAGGAGCTCTATGAAACAAATCAGATTTCACACTATGAATTCGAAAAA
TTTGATCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

49.39

100

0.502
  comGA Staphylococcus aureus N315

49.39

100

0.502
  comGA Bacillus subtilis subsp. subtilis str. 168

37.73

100

0.381
  pilB Glaesserella parasuis strain SC1401

34.012

100

0.362
  pilB Vibrio parahaemolyticus RIMD 2210633

34.513

100

0.362