Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KFV05_RS06905 Genome accession   NZ_CP073799
Coordinates   1355196..1356167 (-) Length   323 a.a.
NCBI ID   WP_164942281.1    Uniprot ID   -
Organism   Macrococcoides canis strain Epi0089     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1350196..1361167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFV05_RS06870 (KFV05_06875) gcvT 1350891..1351976 (-) 1086 WP_211554736.1 glycine cleavage system aminomethyltransferase GcvT -
  KFV05_RS06875 (KFV05_06880) - 1352114..1352620 (-) 507 WP_252455208.1 shikimate kinase -
  KFV05_RS06880 (KFV05_06885) - 1352781..1353233 (-) 453 WP_210151817.1 competence type IV pilus minor pilin ComGF -
  KFV05_RS06885 (KFV05_06890) - 1353178..1353420 (-) 243 WP_158290712.1 hypothetical protein -
  KFV05_RS06890 (KFV05_06895) comGD 1353407..1353832 (-) 426 WP_254245865.1 competence type IV pilus minor pilin ComGD -
  KFV05_RS06895 (KFV05_06900) comGC 1353825..1354157 (-) 333 WP_174804787.1 competence type IV pilus major pilin ComGC -
  KFV05_RS06900 (KFV05_06905) comGB 1354172..1355221 (-) 1050 WP_254245866.1 competence type IV pilus assembly protein ComGB -
  KFV05_RS06905 (KFV05_06910) comGA 1355196..1356167 (-) 972 WP_164942281.1 competence type IV pilus ATPase ComGA Machinery gene
  KFV05_RS06910 (KFV05_06915) - 1356380..1356628 (+) 249 WP_086042733.1 DUF2626 domain-containing protein -
  KFV05_RS06915 (KFV05_06920) - 1356656..1357276 (-) 621 WP_254245867.1 MBL fold metallo-hydrolase -
  KFV05_RS06920 (KFV05_06925) - 1357366..1357605 (+) 240 WP_174804788.1 DUF2759 domain-containing protein -
  KFV05_RS06925 (KFV05_06930) - 1357632..1357943 (-) 312 WP_254245868.1 MTH1187 family thiamine-binding protein -
  KFV05_RS06930 (KFV05_06935) - 1357943..1358911 (-) 969 WP_210151820.1 ROK family glucokinase -
  KFV05_RS06935 (KFV05_06940) - 1358908..1359099 (-) 192 WP_086042738.1 YqgQ family protein -
  KFV05_RS06940 (KFV05_06945) - 1359104..1360552 (-) 1449 WP_254245869.1 rhomboid family protein -
  KFV05_RS06945 (KFV05_06950) - 1360528..1361082 (-) 555 WP_254245870.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 37275.84 Da        Isoelectric Point: 6.5751

>NTDB_id=560772 KFV05_RS06905 WP_164942281.1 1355196..1356167(-) (comGA) [Macrococcoides canis strain Epi0089]
MEQLFNEIIEEAIIQSASDIHFIPNDKSISIKFRIHGDIEDYREIDDMLFRKLLSYIKFTAHLDVSEKNKAQSGIIQFNL
KDLRYNIRASTLPRSLGDEACVLRIIRQNFIDEYQTNDKILYNQIKKSSGIIIISGPTGSGKSTLMYQLVHYARNTLKRQ
IISIEDPVEQHIEGIIQVNVNEKAEITYHTAIKAILRCDPDIIMLGEIRDSVVAHQVINAGLSGHLVLTTLHANDCIGAL
FRLKEMGINAVDLYQSINLIINQRLVRKKDGMGRLLAYEFLTKKDIEKYLQGEHINYRTLTDQFKELYETNQISHYEFEK
FDL

Nucleotide


Download         Length: 972 bp        

>NTDB_id=560772 KFV05_RS06905 WP_164942281.1 1355196..1356167(-) (comGA) [Macrococcoides canis strain Epi0089]
ATGGAACAGTTATTCAATGAAATAATAGAGGAAGCAATTATACAAAGTGCATCAGATATTCACTTCATACCAAACGATAA
AAGCATATCTATCAAATTTAGGATACATGGTGATATTGAAGACTATAGAGAAATCGATGACATGTTATTCAGGAAATTAC
TTTCTTATATTAAATTTACAGCACATCTTGATGTATCAGAAAAGAATAAGGCACAGAGTGGAATCATCCAATTTAATCTG
AAAGACTTAAGATATAATATTCGCGCATCTACTTTACCTCGATCATTAGGCGATGAAGCATGTGTACTGAGAATCATCAG
ACAAAATTTTATAGATGAATATCAGACGAATGACAAGATATTGTACAATCAAATAAAAAAATCAAGCGGTATTATCATCA
TAAGTGGACCAACTGGAAGCGGGAAAAGCACATTGATGTATCAGCTTGTACATTATGCGAGGAACACATTGAAGAGACAA
ATAATATCAATAGAAGATCCTGTTGAACAGCATATTGAAGGAATTATACAGGTTAATGTTAACGAAAAAGCAGAGATAAC
GTATCATACTGCGATAAAGGCAATCCTTAGATGTGACCCGGATATAATTATGCTAGGTGAAATCAGAGATTCAGTCGTAG
CACATCAAGTGATCAATGCAGGACTAAGCGGTCATCTCGTTTTAACGACATTACATGCGAATGATTGCATCGGTGCATTA
TTTCGGTTAAAAGAAATGGGTATTAATGCTGTTGATCTATATCAAAGTATCAATCTGATTATTAATCAAAGGCTTGTAAG
AAAAAAAGATGGGATGGGGCGTCTATTGGCCTATGAATTTTTAACAAAAAAGGATATTGAAAAATATTTACAAGGTGAAC
ATATTAACTATAGAACGTTGACCGATCAATTTAAGGAGCTCTATGAAACAAATCAGATTTCACACTATGAATTCGAAAAA
TTTGATCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

49.39

100

0.502
  comGA Staphylococcus aureus N315

49.39

100

0.502
  comGA Bacillus subtilis subsp. subtilis str. 168

37.73

100

0.381
  pilB Glaesserella parasuis strain SC1401

34.302

100

0.365
  pilB Vibrio parahaemolyticus RIMD 2210633

35.03

100

0.362