Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KFV04_RS06605 Genome accession   NZ_CP073798
Coordinates   1316107..1317078 (-) Length   323 a.a.
NCBI ID   WP_236365614.1    Uniprot ID   -
Organism   Macrococcoides canis strain KM2258     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1311107..1322078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFV04_RS06570 (KFV04_06550) gcvT 1311804..1312889 (-) 1086 WP_086043664.1 glycine cleavage system aminomethyltransferase GcvT -
  KFV04_RS06575 (KFV04_06555) - 1313027..1313533 (-) 507 WP_086042649.1 shikimate kinase -
  KFV04_RS06580 (KFV04_06560) - 1313694..1314146 (-) 453 WP_254254545.1 competence type IV pilus minor pilin ComGF -
  KFV04_RS06585 (KFV04_06565) - 1314091..1314333 (-) 243 WP_236365610.1 hypothetical protein -
  KFV04_RS06590 (KFV04_06570) comGD 1314320..1314766 (-) 447 WP_236365611.1 competence type IV pilus minor pilin ComGD -
  KFV04_RS06595 (KFV04_06575) comGC 1314738..1315070 (-) 333 WP_254254546.1 competence type IV pilus major pilin ComGC -
  KFV04_RS06600 (KFV04_06580) comGB 1315083..1316132 (-) 1050 WP_254254547.1 competence type IV pilus assembly protein ComGB -
  KFV04_RS06605 (KFV04_06585) comGA 1316107..1317078 (-) 972 WP_236365614.1 competence type IV pilus ATPase ComGA Machinery gene
  KFV04_RS06610 (KFV04_06590) - 1317290..1317538 (+) 249 WP_086042733.1 DUF2626 domain-containing protein -
  KFV04_RS06615 (KFV04_06595) - 1317566..1318186 (-) 621 WP_086042734.1 MBL fold metallo-hydrolase -
  KFV04_RS06620 (KFV04_06600) - 1318276..1318515 (+) 240 WP_174804788.1 DUF2759 domain-containing protein -
  KFV04_RS06625 (KFV04_06605) - 1318542..1318853 (-) 312 WP_086042736.1 MTH1187 family thiamine-binding protein -
  KFV04_RS06630 (KFV04_06610) - 1318853..1319821 (-) 969 WP_236365616.1 ROK family glucokinase -
  KFV04_RS06635 (KFV04_06615) - 1319818..1320009 (-) 192 WP_086042738.1 YqgQ family protein -
  KFV04_RS06640 (KFV04_06620) - 1320014..1321441 (-) 1428 WP_254254548.1 rhomboid family protein -
  KFV04_RS06645 (KFV04_06625) - 1321438..1321992 (-) 555 WP_157891076.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 37271.88 Da        Isoelectric Point: 6.7240

>NTDB_id=560742 KFV04_RS06605 WP_236365614.1 1316107..1317078(-) (comGA) [Macrococcoides canis strain KM2258]
MEKLFNEIIEEAIIQGASDIHFIPNDKSISIKFRIHGDIEDYREIDDMLFRKLLSYIKFTAHLDVSEKNKAQSGIIHFNL
KDLRYNIRASTLPRALGDEACVLRIIRQNFIDEYQTDDKILFNQIKKSSGIIIISGPTGSGKSTLMYQLVHYARNTLKRQ
IISIEDPVEQHIEGIIQVNVNEKAEITYHTAIKAILRCDPDIIMLGEIRDSVVAQQVINAGLSGHLVLTTLHANDCIGAL
FRLKEMGINTVDLYQSINLIINQRLVRKKDGKGRLLTYEFLTKKDIEKYLQGEHINYRTLTDQFKELYETNQISHYEFEK
FDL

Nucleotide


Download         Length: 972 bp        

>NTDB_id=560742 KFV04_RS06605 WP_236365614.1 1316107..1317078(-) (comGA) [Macrococcoides canis strain KM2258]
ATGGAAAAATTATTCAATGAAATAATAGAGGAAGCAATTATACAAGGTGCATCAGATATTCACTTCATACCAAACGATAA
AAGCATATCTATCAAATTTAGGATACATGGTGATATTGAAGACTATAGAGAAATCGACGACATGTTATTCAGGAAATTAC
TTTCTTATATTAAATTTACGGCACATCTTGACGTATCAGAAAAGAATAAGGCACAGAGTGGAATCATCCATTTTAATCTG
AAAGACTTAAGATATAATATTCGCGCATCTACTTTACCTCGAGCATTAGGCGATGAAGCATGTGTACTGAGAATCATCAG
ACAAAATTTTATTGATGAATATCAGACGGATGACAAGATATTGTTCAATCAAATAAAAAAATCAAGCGGTATTATCATCA
TAAGTGGACCAACTGGAAGCGGGAAAAGCACATTGATGTACCAGCTTGTACATTATGCGAGGAACACATTGAAGAGACAA
ATAATATCAATAGAAGATCCTGTTGAACAGCATATTGAAGGAATTATACAAGTTAATGTTAACGAAAAAGCAGAGATAAC
ATATCATACCGCGATAAAGGCAATCCTTAGATGTGATCCGGATATCATTATGCTAGGTGAAATCAGAGATTCAGTCGTAG
CACAGCAAGTGATCAATGCAGGACTAAGCGGTCATCTCGTTTTAACGACATTACATGCGAATGATTGCATCGGTGCATTA
TTTCGGTTAAAAGAAATGGGTATTAATACTGTTGATCTATATCAAAGTATCAATCTGATTATTAATCAAAGGCTTGTAAG
AAAGAAAGATGGGAAGGGGCGTCTATTGACCTATGAATTTTTGACAAAAAAGGATATTGAAAAATATTTACAAGGTGAAC
ATATTAACTATAGAACGTTGACCGATCAATTTAAGGAGCTCTATGAAACAAATCAGATTTCACACTATGAATTCGAAAAA
TTTGATCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

48.78

100

0.495
  comGA Staphylococcus aureus N315

48.78

100

0.495
  comGA Bacillus subtilis subsp. subtilis str. 168

38.037

100

0.384
  pilB Glaesserella parasuis strain SC1401

34.012

100

0.362
  pilB Vibrio parahaemolyticus RIMD 2210633

34.513

100

0.362