Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   KEF49_RS05560 Genome accession   NZ_CP073635
Coordinates   1127859..1129688 (+) Length   609 a.a.
NCBI ID   WP_013351834.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Ba13     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1122859..1134688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEF49_RS05530 (KEF49_05530) - 1123522..1124100 (+) 579 WP_013351830.1 GNAT family N-acetyltransferase -
  KEF49_RS05535 (KEF49_05535) spx 1124278..1124673 (+) 396 WP_003327578.1 transcriptional regulator Spx -
  KEF49_RS05540 (KEF49_05540) - 1124731..1125387 (-) 657 WP_065981171.1 TerC family protein -
  KEF49_RS05545 (KEF49_05545) - 1125570..1125672 (+) 103 Protein_1040 hypothetical protein -
  KEF49_RS05550 (KEF49_05550) mecA 1125663..1126319 (+) 657 WP_212100921.1 adaptor protein MecA Regulator
  KEF49_RS05555 (KEF49_05555) - 1126470..1127630 (+) 1161 WP_212100924.1 competence protein CoiA family protein -
  KEF49_RS05560 (KEF49_05560) pepF 1127859..1129688 (+) 1830 WP_013351834.1 oligoendopeptidase F Regulator
  KEF49_RS05565 (KEF49_05565) - 1129724..1129891 (-) 168 WP_016938224.1 hypothetical protein -
  KEF49_RS05570 (KEF49_05570) spxH 1130176..1131078 (-) 903 WP_013351835.1 protease adaptor protein SpxH -
  KEF49_RS05575 (KEF49_05575) - 1131075..1131473 (-) 399 WP_212100927.1 thiol management oxidoreductase -
  KEF49_RS05580 (KEF49_05580) - 1131698..1132384 (-) 687 WP_212100929.1 lytic transglycosylase domain-containing protein -
  KEF49_RS05585 (KEF49_05585) - 1132389..1132961 (-) 573 WP_065981176.1 CYTH domain-containing protein -
  KEF49_RS05590 (KEF49_05590) - 1133086..1133451 (+) 366 WP_013351839.1 hypothetical protein -
  KEF49_RS05595 (KEF49_05595) yjbM 1133479..1134114 (+) 636 WP_013351840.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69744.39 Da        Isoelectric Point: 5.4370

>NTDB_id=559578 KEF49_RS05560 WP_013351834.1 1127859..1129688(+) (pepF) [Bacillus amyloliquefaciens strain Ba13]
MTEENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTSRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKVYSHAIEEITKQRPHVLSEK
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSDREVRKNAFKAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNNIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHNYDLYTPLVKDSGM
KVTYEEAKDYMLKGLAPLGDEYASILKEGLENRWVDVYENKGKRSGAYSSGTYGTNPYILMNWHDTVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNKLYYDLNKKYFGDNMVIDEEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTNFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=559578 KEF49_RS05560 WP_013351834.1 1127859..1129688(+) (pepF) [Bacillus amyloliquefaciens strain Ba13]
ATGACTGAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATTCCGAATTTATCTTCCTTTAAAGGCA
CGCTTGCTCATTCAGCCGATAATTTATACGCGGCGCTTACCGCACAGGATGAAGTGACGAGCCGTCTGGGAAAACTTTAC
GCATACGCGCATATGCGCTCAGATCAGGATACGGGAAATTCTTTCTACCAAGGTCTGAACGACAAAGCCGCAAGTCTGTA
TTCTCAGGCCGCCAGTGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCATCAGTTTATTC
TGGAAAAAGAAGAGCTTAAGGTTTACTCCCATGCCATTGAAGAAATTACAAAACAGCGTCCGCACGTGCTGAGCGAAAAA
GAAGAGGCCCTGCTTGCGGAAGCGTCAGAAGCGCTGTCTTCACCTTCCAATACATTCAGTGTTTTAAACAACGCCGATAT
TACGTTTCCGTCGATAAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTTCTGGAAAGCA
GCGATCGTGAAGTCCGTAAAAACGCCTTTAAAGCAGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTGAAAAAAGATAATTTCTACGCCCGGGTGAAAAAATACAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAACATCCCTGAAGAAGTTTATGATAACCTTATCAAAACGATCAATAAACATCTTCCGTTATTACACCGTTACATTG
ATCTGCGGAAAAAGGTGCTCGGCCTTGATGAAGTCCACAATTATGACCTGTACACACCGCTTGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCAAAAGATTATATGCTCAAAGGGCTTGCTCCGTTAGGCGATGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATGTATACGAAAACAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAACCCGTACATCTTAATGAACTGGCACGACACTGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCGTACGGAAACTACAGCATTTTTGTCGCTGAAGTCGCTTCTAC
GACCAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAAGATGAAAAACAGCGTCTGTATCTTCTCAACCACA
TGCTTGAAGGTTTCAGAGGCACAGTCTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAA
GAAGGCGAGCCGTTAACGCCTGAACTGCTGAACAAGCTTTATTATGATCTCAATAAAAAGTATTTCGGAGACAACATGGT
AATTGATGAAGAAATCAGCCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTATATCAATATGCGACAG
GCTACAGTGCGGCTCAAGCGCTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTTGAGCGTTATACCAACTTCTTA
AAAGCCGGAAGCTCAGAATATCCGATCGACATCCTGAAAAAAGCGGGAGTCGATATGACATCGCCTGAACCGATCGAAGC
TGCCTGCAAAATGTTTGAAGAGAAACTGAATGAAATGGAAGAACTCCTTTCGAAAGTGAATCCTTCCTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50

97.537

0.488