Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KDW96_RS09740 Genome accession   NZ_CP073346
Coordinates   2083797..2084861 (-) Length   354 a.a.
NCBI ID   WP_255840209.1    Uniprot ID   -
Organism   Pseudomonas benzenivorans strain D13-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2078797..2089861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KDW96_RS09715 aroK 2079429..2079947 (-) 519 WP_255840205.1 shikimate kinase AroK -
  KDW96_RS09720 (KDW96_09685) pilQ 2079951..2082029 (-) 2079 WP_255840506.1 type IV pilus secretin PilQ Machinery gene
  KDW96_RS09725 (KDW96_09690) pilP 2082084..2082608 (-) 525 WP_255840206.1 type 4a pilus biogenesis lipoprotein PilP -
  KDW96_RS09730 (KDW96_09695) pilO 2082605..2083228 (-) 624 WP_255840207.1 type 4a pilus biogenesis protein PilO -
  KDW96_RS09735 (KDW96_09700) pilN 2083225..2083797 (-) 573 WP_255840208.1 type 4a pilus biogenesis protein PilN -
  KDW96_RS09740 (KDW96_09705) pilM 2083797..2084861 (-) 1065 WP_255840209.1 pilus assembly protein PilM Machinery gene
  KDW96_RS09745 (KDW96_09710) - 2085046..2087466 (+) 2421 WP_370295410.1 penicillin-binding protein 1A -
  KDW96_RS09750 (KDW96_09715) - 2087557..2088825 (-) 1269 WP_255840211.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37901.13 Da        Isoelectric Point: 4.3813

>NTDB_id=559095 KDW96_RS09740 WP_255840209.1 2083797..2084861(-) (pilM) [Pseudomonas benzenivorans strain D13-4]
MLGLFNKNANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLSKAKTSARSVAV
AVAGSAVITKTIEMEAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGVSPRNGERVEVLLAACRKENVEVREAALAL
SGLTAKVVDVEAYALERAYSLLAEQLGSGQEELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALGGKVNAGALASDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=559095 KDW96_RS09740 WP_255840209.1 2083797..2084861(-) (pilM) [Pseudomonas benzenivorans strain D13-4]
GTGCTAGGGCTCTTCAATAAGAATGCGAATACGCTGCTGGGGATCGATATCAGTTCGACCTCGGTCAAGCTCCTCGAATT
GAGTCGCTCCGGAAGCCGCTACAAGGTAGAGGCTTACGCAGTCGAGCCACTCCCCCCAAACGCAGTGGTCGAGAAGAACA
TCGCCGAACTGGAAGGTGTCGGCCAGGCCCTCTCTCGCGTACTGAGCAAGGCCAAGACCAGCGCCAGATCCGTCGCCGTG
GCAGTGGCCGGCTCGGCAGTGATCACCAAGACCATCGAAATGGAAGCCGGGCTTTCTGACGATGAGCTGGAGAACCAGCT
GAAGATCGAGGCGGACCAATACATTCCGTACCCGCTCGAGGAAGTAGCGATCGACTTCGAGGTGCAAGGCGTATCGCCTC
GCAACGGTGAGCGGGTCGAGGTGCTGCTGGCCGCCTGTCGCAAAGAGAACGTCGAAGTCCGGGAGGCGGCGCTGGCCTTG
TCTGGCCTGACGGCCAAGGTTGTCGACGTCGAGGCCTATGCCTTGGAGCGGGCGTACAGCCTTCTTGCGGAGCAACTCGG
CAGCGGCCAGGAAGAGCTGACAGTCGCCGTAGTCGACATCGGTGCGACCATGACGACCCTGAGCGTTCTGCATAACGGTC
GCACCATCTACACCCGGGAGCAGTTGTTCGGCGGCAAGCAGCTGACCGAGGAGATCCAGCGGCGTTATGGCCTCTCTGTT
GAAGAGGCGGGACTGGCCAAGAAGCAGGGCGGCCTGCCGGATGACTACGACAGCGAGGTGTTGCAGCCCTTTAAAGAGGC
GGTCGTGCAGCAGGTTTCGCGTTCCTTGCAGTTCTTCTTCGCCGCAGGACAGTTCAATGATGTCGACTACATCCTGCTGG
CGGGTGGCACCGCTTCAATTCCCGATCTGGATCGGCTGATTCAACAGAAGATCGGCACGCAGACGCTGGTGGCCAACCCG
TTCGCGGACATGGCGCTGGGCGGCAAGGTCAATGCGGGAGCCCTCGCCAGTGATGCACCATCGTTGATGATTGCCTGTGG
GCTGGCCATGAGGAGTTTTGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

45.763

100

0.458