Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KDW96_RS09720 Genome accession   NZ_CP073346
Coordinates   2079951..2082029 (-) Length   692 a.a.
NCBI ID   WP_255840506.1    Uniprot ID   -
Organism   Pseudomonas benzenivorans strain D13-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2074951..2087029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KDW96_RS09705 (KDW96_09675) - 2076683..2078263 (-) 1581 WP_255840203.1 SPOR domain-containing protein -
  KDW96_RS09710 (KDW96_09680) aroB 2078271..2079377 (-) 1107 WP_255840204.1 3-dehydroquinate synthase -
  KDW96_RS09715 aroK 2079429..2079947 (-) 519 WP_255840205.1 shikimate kinase AroK -
  KDW96_RS09720 (KDW96_09685) pilQ 2079951..2082029 (-) 2079 WP_255840506.1 type IV pilus secretin PilQ Machinery gene
  KDW96_RS09725 (KDW96_09690) pilP 2082084..2082608 (-) 525 WP_255840206.1 type 4a pilus biogenesis lipoprotein PilP -
  KDW96_RS09730 (KDW96_09695) pilO 2082605..2083228 (-) 624 WP_255840207.1 type 4a pilus biogenesis protein PilO -
  KDW96_RS09735 (KDW96_09700) pilN 2083225..2083797 (-) 573 WP_255840208.1 type 4a pilus biogenesis protein PilN -
  KDW96_RS09740 (KDW96_09705) pilM 2083797..2084861 (-) 1065 WP_255840209.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74399.37 Da        Isoelectric Point: 4.6594

>NTDB_id=559094 KDW96_RS09720 WP_255840506.1 2079951..2082029(-) (pilQ) [Pseudomonas benzenivorans strain D13-4]
MNSSLSRIGISLSAMLLSPALLAADLQALDVAALPGDRVELKLSFDEPVPAPRGYTIEQPARIALDLPGVSNKLGAKNRE
LGVGNARSVTVVEAKDRTRLIINLTNLAPYSTRVDGSNLYVLVGDAGNRSGAAAQPLATSTPVAVAPAAKVYAPQRKAIS
DIDFQRGEQGEGNVVITLSDASVSPDIQEQGGKIRLDFAKTQLPEALRVRLDVKDFATPVQFVSATGTADRTSIIIEPTG
LYDYLAYQTDNKLTLSIKPLTEDEAEKRKNDRFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQN
VPWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADMAKLFQSVTSAEG
SSDERGSITVDDRTNSIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKALGVEWRGVSLGDNNFVVGGTN
SLIKGSDPLDFQAGTFVDLGAAGANTGLNLGFITNNTLLDLELSAMEKTGNGEVVSQPKVVTSDKETAKILKGAEVPYQE
ASSSGATTTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFSNAAASGGVPAINKNEVNAKVLVADGETIVIGGVFSN
TQSKSVDKVPLLGDIPFIGAMFRRDVVKDSKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=559094 KDW96_RS09720 WP_255840506.1 2079951..2082029(-) (pilQ) [Pseudomonas benzenivorans strain D13-4]
ATGAATAGCTCCCTTTCACGTATAGGTATTTCTCTGTCGGCGATGTTGTTGTCGCCGGCGCTGTTGGCAGCGGACCTGCA
AGCGCTCGATGTGGCCGCCTTGCCTGGCGACAGGGTTGAATTGAAGCTGTCGTTTGATGAGCCCGTTCCCGCACCACGGG
GTTACACGATCGAACAGCCCGCGCGTATCGCTTTGGATTTGCCTGGCGTCTCCAATAAGTTGGGAGCAAAGAATCGCGAG
CTGGGCGTGGGTAACGCGCGCAGCGTGACAGTGGTCGAGGCCAAGGACCGCACTCGGTTGATCATCAATCTGACCAATCT
GGCTCCTTATAGTACTCGCGTCGACGGCTCAAATCTTTACGTGCTTGTCGGTGATGCTGGCAATAGAAGCGGCGCCGCCG
CGCAGCCTCTAGCGACATCGACGCCTGTGGCGGTAGCTCCAGCTGCCAAGGTTTACGCGCCGCAGCGCAAGGCCATTAGT
GATATCGACTTTCAACGAGGCGAGCAGGGCGAGGGTAATGTTGTTATTACTTTGTCCGACGCGTCGGTGAGCCCGGATAT
CCAGGAGCAAGGTGGTAAAATCCGCTTGGACTTCGCGAAGACTCAACTGCCTGAGGCCTTACGCGTGCGCCTGGATGTGA
AGGACTTCGCCACTCCCGTACAATTTGTGAGTGCGACGGGTACTGCAGATAGAACAAGTATTATTATCGAGCCGACAGGG
CTCTACGATTACTTGGCTTATCAGACAGATAATAAGTTGACGCTTAGTATCAAGCCGTTGACTGAAGATGAGGCCGAGAA
ACGTAAGAATGACCGCTTTGCGTATACCGGTGAAAAGCTGTCTCTGAATTTTCAAGACATCGATGTGCGCTCGGTTCTGC
AGTTGATCGCTGACTTTACTGATTTGAACTTGGTTGCAAGTGATACTGTGCAAGGCAATATTACCTTGCGGTTGCAAAAC
GTTCCGTGGGACCAGGCTCTGGACTTGGTGTTGAAAACGAAGGGGTTAGACAAGCGTCAGGTCGGCAATGTGTTGCTGGT
GGCGCCGGCGGACGAAATAGCCGCCCGCGAGCGCCAGGAGCTGGAGGCGCAGAAGCAGATCGCCGAACTGGCCCCATTGC
GCCGTGAGCTGATTCAGGTCAACTACGCCAAGGCCGCTGACATGGCTAAGTTATTCCAGTCGGTGACCAGCGCAGAAGGA
AGCTCCGATGAGCGCGGGTCTATTACGGTGGATGATCGGACCAACAGCATTATCGCCTATCAAACCCAGGACCGGCTGGA
CGAGTTGCGCCGCATTGTGTCCCAGCTGGATATCCCGGTTCGACAAGTGATGATTGAGGCGCGAATTGTTGAAGCCAACG
TCGATTATGACAAGGCCCTCGGTGTTGAGTGGCGCGGGGTAAGCCTTGGAGACAATAACTTTGTCGTGGGCGGGACCAAT
AGTCTGATTAAGGGTTCGGATCCGCTTGACTTCCAGGCGGGTACCTTCGTCGATCTAGGTGCGGCAGGAGCTAATACCGG
CCTCAACCTAGGCTTTATTACCAACAATACGTTGCTGGATCTTGAGCTCTCGGCCATGGAGAAAACCGGTAACGGGGAAG
TGGTGTCGCAGCCTAAGGTGGTCACCTCGGATAAGGAAACTGCAAAAATATTAAAAGGTGCAGAAGTCCCGTATCAGGAG
GCCAGCTCCAGTGGTGCGACTACTACCTCGTTCAAGGAAGCTGCGCTGTCTCTCGAGGTTACACCTCAGATCACGCCCGA
CAACCGGATTATCATGGAGGTCAAGGTCACCAAGGATGCGCCCGACTTCTCAAACGCTGCGGCGAGCGGCGGGGTGCCGG
CAATCAACAAGAATGAGGTGAACGCTAAGGTGCTGGTGGCTGACGGCGAGACGATAGTAATTGGTGGCGTGTTCTCCAAT
ACGCAAAGTAAGTCGGTCGACAAGGTGCCATTGTTGGGCGACATCCCGTTCATTGGGGCAATGTTTCGGCGCGATGTCGT
CAAGGACAGTAAGTCGGAGCTGCTGGTCTTTCTCACTCCGCGTATCATGAACAATCAGGCCATCTCTGTGAGCCGCTAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(38-121)

Bacterial type II and III secretion system protein (Secretin)

(525-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.173

100

0.783
  comQ Acinetobacter baylyi ADP1

42.486

100

0.425
  pilQ Acinetobacter baumannii strain A118

41.311

100

0.419
  pilQ Acinetobacter baumannii D1279779

41.311

100

0.419
  pilQ Legionella pneumophila strain Lp02

37.592

98.41

0.37
  pilQ Legionella pneumophila strain ERS1305867

37.592

98.41

0.37