Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   KCX77_RS13265 Genome accession   NZ_CP073265
Coordinates   2658082..2658705 (-) Length   207 a.a.
NCBI ID   WP_212063633.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain CNY01     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2653082..2663705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCX77_RS13235 (KCX77_13235) rpsT 2653376..2653642 (+) 267 WP_003325396.1 30S ribosomal protein S20 -
  KCX77_RS13240 (KCX77_13240) holA 2653657..2654700 (-) 1044 WP_010789253.1 DNA polymerase III subunit delta -
  KCX77_RS13245 (KCX77_13245) - 2654740..2654889 (-) 150 WP_010789254.1 hypothetical protein -
  KCX77_RS13250 (KCX77_13250) - 2654930..2655064 (+) 135 WP_003229983.1 YqzM family protein -
  KCX77_RS13255 (KCX77_13255) comEC 2655105..2657438 (-) 2334 WP_212063632.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  KCX77_RS13260 (KCX77_13260) - 2657445..2658014 (-) 570 WP_003325392.1 ComE operon protein 2 -
  KCX77_RS13265 (KCX77_13265) comEA 2658082..2658705 (-) 624 WP_212063633.1 helix-hairpin-helix domain-containing protein Machinery gene
  KCX77_RS13270 (KCX77_13270) comER 2658789..2659610 (+) 822 WP_003325389.1 late competence protein ComER -
  KCX77_RS13275 (KCX77_13275) - 2659680..2660420 (-) 741 WP_003325388.1 class I SAM-dependent methyltransferase -
  KCX77_RS13280 (KCX77_13280) rsfS 2660417..2660773 (-) 357 WP_003325387.1 ribosome silencing factor -
  KCX77_RS13285 (KCX77_13285) yqeK 2660790..2661350 (-) 561 WP_010789259.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  KCX77_RS13290 (KCX77_13290) - 2661340..2661909 (-) 570 WP_003325385.1 nicotinate-nucleotide adenylyltransferase -
  KCX77_RS13295 (KCX77_13295) yhbY 2661921..2662211 (-) 291 WP_003325384.1 ribosome assembly RNA-binding protein YhbY -
  KCX77_RS13300 (KCX77_13300) aroE 2662205..2663047 (-) 843 WP_010789260.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 21989.77 Da        Isoelectric Point: 4.6181

>NTDB_id=558647 KCX77_RS13265 WP_212063633.1 2658082..2658705(-) (comEA) [Bacillus atrophaeus strain CNY01]
MNWFYQHKKIILTAGAAVIFIVILLNFPGAKKQEPVQQITASPDTAGTQVQQQESKGDADGEKIIIDIKGAVKHPGVYEL
KTGDRVSQAIEKAGGINSEADEKQVNLAELLQDGTVVYIPSEGEEPTQSAVANASVQNGAGKEALVNINTASLEELQAIS
GVGPSKAEAIIAYREENGKFQAVEDITNVSGIGEKSFEKIKSAITVK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=558647 KCX77_RS13265 WP_212063633.1 2658082..2658705(-) (comEA) [Bacillus atrophaeus strain CNY01]
ATGAATTGGTTTTACCAGCACAAAAAAATAATCTTAACGGCAGGTGCTGCTGTCATCTTCATCGTCATCCTGCTTAATTT
TCCCGGCGCAAAGAAGCAGGAGCCTGTTCAGCAAATCACAGCGTCGCCTGACACGGCGGGCACACAAGTTCAGCAGCAAG
AATCGAAAGGTGATGCTGACGGAGAAAAAATTATCATTGATATTAAAGGCGCTGTCAAACATCCGGGTGTTTACGAATTG
AAAACGGGAGACAGAGTTTCACAGGCTATCGAAAAAGCGGGAGGCATTAATTCGGAGGCTGATGAAAAACAAGTAAATTT
AGCTGAACTCCTGCAGGACGGCACTGTCGTGTACATTCCGAGTGAGGGGGAAGAGCCAACACAAAGCGCTGTCGCAAATG
CTTCTGTGCAAAACGGAGCAGGAAAAGAGGCTTTGGTTAATATCAATACAGCGTCATTAGAGGAACTGCAGGCTATTTCC
GGGGTCGGTCCGTCAAAAGCAGAAGCTATAATAGCTTATCGTGAAGAAAACGGAAAATTTCAAGCCGTTGAAGACATTAC
AAATGTCTCGGGAATCGGCGAAAAATCATTTGAGAAAATAAAATCAGCCATCACAGTTAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(66-120)

Helix-hairpin-helix motif (HHH_3)

(143-205)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

65.7

100

0.657
  comEA Staphylococcus aureus MW2

36.232

100

0.362