Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   KCX77_RS06260 Genome accession   NZ_CP073265
Coordinates   1238585..1242289 (+) Length   1234 a.a.
NCBI ID   WP_212064410.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain CNY01     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1233585..1247289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCX77_RS06245 (KCX77_06245) - 1234032..1234358 (-) 327 WP_212064408.1 four-helix bundle copper-binding protein -
  KCX77_RS06250 (KCX77_06250) - 1234455..1234892 (-) 438 WP_212064409.1 ferritin family protein -
  KCX77_RS06255 (KCX77_06255) addB 1235098..1238598 (+) 3501 WP_063637346.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  KCX77_RS06260 (KCX77_06260) addA 1238585..1242289 (+) 3705 WP_212064410.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  KCX77_RS06265 (KCX77_06265) sbcD 1242360..1243538 (+) 1179 WP_212064411.1 exonuclease subunit SbcD -
  KCX77_RS06270 (KCX77_06270) sbcC 1243535..1246924 (+) 3390 WP_212064412.1 exonuclease subunit SbcC -
  KCX77_RS06275 (KCX77_06275) - 1246941..1247243 (+) 303 WP_010788170.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141506.08 Da        Isoelectric Point: 5.4650

>NTDB_id=558614 KCX77_RS06260 WP_212064410.1 1238585..1242289(+) (addA) [Bacillus atrophaeus strain CNY01]
MQIPKPADSTWTDDQWNAIVSSGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAEA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLRKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPKTWLEGFVHLYDASEKSTIEELPFYPYMKEDIAMVLSAVKEKLQRA
LELTKVPGGPAPRADNFLDDLAQTEDLIAHSADFTELYKRVPAVSFKRAKAVKGDEFDQALTEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLSDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAVNESGERKPSEAAAYYQEQ
FQEVLVDEYQDTNLVQEAILQLVTSGPEASGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTETGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKVGEVDYDEQAELKLGASYPPNDQTETELLLIDRAEEKEASEETEELETVQLEAKAIAKQIR
KLVSSPYKVYDGKSKTHSNIRYRDIVILLRSMPWAPQIMEELKAQGIPVYANLTSGYFEAVEVSVAISALKVIDNPYQDI
PLASVLRSPIVGADENELSLIRMHNKKAPFYEAMKDYLSGGDRNDELYQKLNTFFELLQKWRSFSKNHSVSELIWEVYRD
SKYMDYVGGMPGGKQRQANLRVLYDRSRQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQASASQPDWLLPDFERYQAKTYLDFIGPALIRHRDMGTDALQGLASQADISEHPARFNIQMVSAQD
LLDDDLEERQEEKSGRLDAIRRGEPVSGSFEFEEQARRRLSWRYPYHDVTKIRTKQSVSEIKRKKEYEDEYSGRSLIRPA
DGNMLYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHAPSKEEAERIVKRLYEKELLTEDQQAVIDLEEIVQFFHTEIGE
KLIGAKWINREVPFSMALSAQDAFLDTESKDEPILVQGIIDCLYETDEGLFLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIELYTKAVEQITKTNVKGRALYFFDGGHVLNV

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=558614 KCX77_RS06260 WP_212064410.1 1238585..1242289(+) (addA) [Bacillus atrophaeus strain CNY01]
ATGCAAATTCCTAAACCGGCAGACAGCACCTGGACAGATGACCAGTGGAACGCCATTGTCTCATCCGGACAGGATATTCT
CGTTGCAGCGGCCGCTGGATCTGGAAAAACCGCTGTGCTTGTTGAGAGAATGATCCGAAAAATTACCGCGGAGGAAAATC
CGATAGATGTGGACCGTCTTTTGGTCGTGACATTTACGAACGCCTCTGCGGCTGAAATGAAGCACCGGATCGCGGAGGCT
TTGGAGAAAGAGCTCGTGAAACGTCCCGGCTCTCTTCATATCAGGCGGCAGCTGTCTCTGTTGAACCGTGCCAGCATTTC
AACCCTTCATTCGTTCTGCTTGCAGGTGCTCAGAAAATATTATTATCTCATTGATCTTGATCCGGGCTTTCGGATTGCCG
ATCAGACAGAAGGCGAGCTGCTCGGGGACGAGGTCCTTGATGAACTGTTTGAAGACGAATACGCGAAGGGTGAAAAAGCG
TTTTTCGAACTGGTGGACCGGTATACGACAGACCGGCACGACTTAGACCTTCAGCACCTCGTGAAACGGGTGTATGAGTT
TTCCCGTTCCCATCCCGATCCAAAAACGTGGCTGGAAGGTTTCGTTCATCTTTACGATGCCTCAGAAAAAAGCACGATTG
AAGAACTGCCGTTTTATCCATATATGAAAGAAGACATTGCGATGGTGCTGAGTGCTGTGAAAGAAAAGCTTCAGCGGGCG
CTTGAGCTGACAAAAGTGCCGGGCGGCCCTGCGCCGCGCGCTGATAATTTTCTGGATGATCTTGCACAGACAGAGGATCT
GATCGCACATTCGGCAGATTTCACTGAATTATACAAGCGGGTTCCCGCAGTCTCCTTTAAGCGTGCCAAAGCGGTCAAAG
GAGATGAATTTGACCAGGCGCTCACAGAAGAAGCGACGGATTTACGGAACGGCGCGAAAAAACTGCTGGAAAAACTAAAA
ACGGACTATTTCACAAGAAGCCCCGAGCAGCATCTGAAGAGCCTGTCGGATATGAAGCCTGTGATTGAAACGCTCGTCCA
GCTTGTCATCAGCTACGGAAAACGGTTTGAAGCCGCCAAACAAGAGAAATCAATTATTGATTTCTCTGACTTGGAGCATT
ACTGCTTGTCTATCCTGACAGCCGTGAATGAGTCGGGGGAGCGCAAGCCGAGTGAGGCGGCGGCTTACTATCAAGAGCAG
TTCCAAGAGGTGCTGGTTGACGAATATCAAGATACGAATCTTGTGCAGGAAGCCATCCTGCAGCTTGTCACAAGCGGACC
TGAAGCTTCAGGAAACTTGTTTATGGTAGGGGACGTCAAACAGTCCATCTACCGATTCAGGCTTGCCGAACCGCTTCTTT
TCCTTTCTAAATATAAACGATTTACCGAAACGGGAGAGGGGACAGGCCGGAAAATTGATTTGAACAAAAACTTTCGAAGC
CGGGCGGATATTTTAGACAGCACGAACTTTCTGTTTAAGCAGCTCATGGGCGGCAAGGTCGGCGAGGTTGATTATGATGA
GCAGGCGGAATTGAAGCTAGGAGCCTCTTACCCTCCGAATGACCAGACTGAAACGGAGCTGTTGCTGATTGACAGAGCGG
AGGAAAAAGAGGCGAGTGAAGAAACAGAAGAGCTCGAAACCGTTCAGCTTGAAGCAAAAGCGATTGCAAAGCAGATTCGA
AAGCTCGTATCCTCTCCTTACAAAGTCTATGATGGAAAATCGAAAACACACAGCAATATCCGCTACCGCGATATCGTGAT
TTTACTGCGTTCCATGCCGTGGGCGCCGCAAATCATGGAAGAACTGAAAGCTCAAGGCATACCCGTCTATGCCAATTTGA
CATCAGGCTATTTTGAAGCGGTTGAGGTGTCTGTTGCGATTTCCGCTTTAAAAGTGATTGATAATCCGTACCAAGATATT
CCTCTCGCTTCCGTGCTGCGCTCGCCGATTGTCGGCGCAGATGAAAATGAACTTTCGCTGATTCGGATGCATAATAAAAA
AGCGCCTTTCTATGAAGCGATGAAGGACTATCTTTCAGGCGGAGACCGAAATGACGAGCTATATCAAAAGCTGAACACGT
TTTTTGAGCTTTTGCAAAAATGGCGTTCTTTTTCGAAAAACCATTCAGTTTCCGAGCTGATATGGGAAGTTTATCGTGAC
AGCAAATATATGGACTATGTCGGCGGTATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCGTTCTCTATGACCGGTC
CCGTCAGTATGAATCGACGGCATTTCGCGGTTTATTTCGTTTCTTGCGGTTTATAGAACGTATGCAGGAACGGGGCGATG
ACCTCGGGACAGCGAGAGCGCTCAGCGAGCAGGAGGACGTGGTCCGCCTGATGACGATTCACAGCAGCAAGGGGCTTGAA
TTCCCTGTCGTATTCGCTGCGGGACTTGGACGGAATTTTAATATGATGGATTTGAATAAATCATATCTGCTGGACAAAGA
GCTTGGTTTCGGCACAAAGTTTATTCACCCCGAATGGCGTATTAGCTATCCGACACTTCCGCTCATCGCCATGAAGAAAA
AAATGCGCCGGGAGCTGCTGTCAGAAGAGCTGCGTGTGCTTTACGTGGCTTTAACAAGAGCGAAGGAAAAGCTGTTTCTT
GTCGGTTCCTGCAAGAATCGTGAGAAGCAGCTGGCGAAATGGCAGGCTTCAGCGTCTCAGCCAGACTGGCTTCTCCCGGA
TTTTGAGCGGTATCAGGCGAAAACGTATTTGGATTTCATCGGTCCCGCATTGATTCGTCACAGAGACATGGGGACAGATG
CTTTACAAGGGCTTGCGAGTCAAGCTGACATATCAGAACACCCTGCCCGGTTTAACATCCAAATGGTCAGCGCCCAAGAT
TTATTGGATGATGATCTGGAAGAAAGACAAGAGGAAAAGAGCGGCCGTCTTGACGCCATCCGCCGCGGTGAGCCTGTGTC
CGGTTCGTTTGAATTCGAAGAACAAGCGCGGCGACGCCTGTCATGGCGATACCCTTATCACGACGTGACAAAAATTCGCA
CGAAGCAGTCTGTTTCCGAGATCAAGAGAAAGAAAGAATACGAGGACGAATACAGCGGCCGCTCTCTCATCAGACCTGCT
GACGGAAACATGTTGTACAGGCGCCCTGCGTTTATGATGAAAAAAGGGCTGACTGCGGCTGAAAAAGGAACGGCGATGCA
CACCGTTATGCAGCATATCCCGCTGACACATGCGCCGTCAAAAGAGGAAGCGGAACGTATTGTCAAACGTCTTTATGAAA
AAGAGCTGCTCACCGAAGACCAGCAAGCCGTTATTGACCTAGAGGAAATCGTGCAATTTTTCCATACTGAAATCGGCGAA
AAGCTGATCGGCGCAAAATGGATTAACCGCGAAGTGCCGTTTAGCATGGCGCTTTCAGCACAAGATGCGTTTCTGGACAC
TGAATCTAAAGATGAGCCTATATTGGTGCAAGGAATTATTGACTGTCTGTATGAGACCGATGAAGGGCTTTTCTTGCTGG
ATTACAAAACGGACCGAATCGAAGGGAAATTCCAGCACGGATTTGAGGGGGCGGCTCCTATTCTTAAAAAACGGTACGAA
ACTCAAATCGAGCTATACACAAAAGCTGTAGAACAAATAACGAAAACCAATGTGAAGGGACGTGCACTCTACTTCTTCGA
TGGAGGACACGTTTTGAACGTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

86.548

100

0.865