Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   KCX77_RS06255 Genome accession   NZ_CP073265
Coordinates   1235098..1238598 (+) Length   1166 a.a.
NCBI ID   WP_063637346.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain CNY01     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1230098..1243598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCX77_RS06230 (KCX77_06230) - 1230519..1232012 (+) 1494 WP_212064407.1 monooxygenase -
  KCX77_RS06235 (KCX77_06235) - 1232096..1232611 (+) 516 WP_106047337.1 MarR family transcriptional regulator -
  KCX77_RS06240 (KCX77_06240) - 1232677..1233825 (-) 1149 WP_010788164.1 AbrB family transcriptional regulator -
  KCX77_RS06245 (KCX77_06245) - 1234032..1234358 (-) 327 WP_212064408.1 four-helix bundle copper-binding protein -
  KCX77_RS06250 (KCX77_06250) - 1234455..1234892 (-) 438 WP_212064409.1 ferritin family protein -
  KCX77_RS06255 (KCX77_06255) addB 1235098..1238598 (+) 3501 WP_063637346.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  KCX77_RS06260 (KCX77_06260) addA 1238585..1242289 (+) 3705 WP_212064410.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  KCX77_RS06265 (KCX77_06265) sbcD 1242360..1243538 (+) 1179 WP_212064411.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134394.22 Da        Isoelectric Point: 5.1681

>NTDB_id=558613 KCX77_RS06255 WP_063637346.1 1235098..1238598(+) (addB) [Bacillus atrophaeus strain CNY01]
MGAEFLVGRSGSGKTKLIIDSIQDELRRNPFGKPIIFLVPDQMTFLMEYELAKTPGIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTSTGIQMLLRKLIEEHKQEFKVYQKASDKSGFTEQVERMLTEFKRYCLEPEDIRRMIESGTATDYRGERVLTD
KLHDLYILYQQMEKSLENQYLHSEDYLTLLAEQIPYAEDLKGAHVYVDGFYQFTPQEFRVLTQLMAQAEHVTFSLTADKP
SHVQAPDELDLFRMTGKTYYQLHQAASELHLDITNRELTETKRYQQTPELAHLEAMYDARPAVPFLEKQEAFTVMQAANR
RTELEGISREIHALVRDKGLRYKDMAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLVEFIRSSLDVIKGNWRY
EAVFRCVKTELLFPLDRPKNIIREQVDQLENYCIAYGIKGDRWTRGDRFVYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFRLQKRMKQAKTVQDMAEALYLYLEETDIPLKLDQERQRAEEDGRIIEAQQHNQAWDAVIQLLEEFVEMMGEDEI
SLSLFQQMMETGAESLKFALIPPALDQVFVGNMDLSRMYGTRCTFVIGANDGVLPARPEDDGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSTVVKRLTELFPEHQERLLTNEPEQVSDCEQLLYLVNKS
VAQSYTASQFRLWLREYDISDVWWSAYNVLMNEPDRLNSKKLFSSLFFRNEVKPLERAVSRQLYGEHIHGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKDQCNKFSYEAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVARVSGILSEHAKASGFAPVGLELGFGGKGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLSEVYYGLALQMLTYLDLSITYSADWLGMKATPAGVLYFHIHDPMIQASVPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEEEFHLLTQHVRRTFQEAGEQITDGCVSIEPYKLKDKTP
CTYCSFKSVCQFDESLEENEYRTLKAEKDHTILDWLKKEGDGDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=558613 KCX77_RS06255 WP_063637346.1 1235098..1238598(+) (addB) [Bacillus atrophaeus strain CNY01]
TTGGGAGCAGAGTTTTTAGTAGGCAGATCCGGGAGTGGAAAAACGAAGCTGATCATAGACAGCATTCAAGATGAGCTGCG
CCGGAATCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCTGGTATAGGCGGAATGATACGCGCTCAAGTGTTCAGCTTTTCACGTTTGGCCTGGCGTGTTCTGCAGCATACGGGC
GGAATGAACCGGCCGTTTGTCACCAGCACGGGAATCCAAATGCTTTTGAGAAAGCTCATTGAAGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGAACAGGTAGAACGGATGCTGACGGAATTTAAACGGTACT
GCCTTGAGCCTGAAGATATCCGCCGCATGATTGAAAGCGGAACTGCCACAGACTATCGCGGCGAGCGGGTGTTAACTGAT
AAGCTGCATGATTTGTATATTTTATATCAGCAGATGGAAAAGAGCCTTGAAAACCAGTATCTTCATTCAGAGGATTATTT
AACGCTGCTGGCGGAACAGATCCCGTATGCGGAAGATTTGAAAGGGGCGCATGTCTATGTAGACGGCTTTTATCAGTTTA
CTCCGCAGGAATTCAGAGTGCTTACGCAGCTTATGGCACAGGCTGAGCATGTGACGTTTTCACTGACGGCGGACAAGCCG
TCCCATGTGCAGGCGCCTGATGAGCTGGATCTGTTCAGAATGACAGGCAAAACATATTATCAGCTCCATCAAGCAGCTTC
GGAGCTTCATTTGGACATCACAAACAGAGAACTGACCGAGACGAAACGATATCAGCAAACACCGGAGCTTGCCCATTTAG
AAGCAATGTATGACGCGCGCCCTGCTGTTCCGTTTTTGGAAAAGCAAGAAGCCTTTACCGTGATGCAGGCTGCGAACAGG
CGGACAGAGCTGGAAGGAATTTCCCGTGAAATTCATGCTCTTGTAAGAGATAAGGGATTACGCTACAAGGATATGGCAAT
TCTCGCCCGCCAGCCGGAAGATTATAAGGATATGGTTAAAGAAGTATTTGCAGATTATGAAATTCCTTACTTTATAGATG
GAAAGGCATCCATGCTGAACCATCCGCTTGTTGAGTTTATCCGTTCAAGCCTTGATGTCATAAAGGGAAATTGGAGATAT
GAAGCGGTGTTTCGCTGTGTGAAAACCGAACTGTTATTCCCGCTTGACCGTCCGAAGAACATCATAAGGGAGCAGGTCGA
TCAACTGGAAAACTATTGTATCGCTTACGGAATTAAAGGCGACCGATGGACGAGAGGCGACCGTTTTGTTTACAGGCGGT
TTGTTTCACTGGATGATGATTTTGCGCAGACAGATCAAGAGATTGAAATGGAAAACATGCTGAATGACACAAGAGACTGG
ATTGTTCCGCCGCTTTTCCGTCTTCAAAAACGGATGAAACAAGCAAAAACGGTCCAAGACATGGCGGAGGCTCTTTATCT
TTATTTAGAAGAAACGGATATTCCGTTAAAGCTGGACCAAGAAAGACAGCGCGCGGAAGAGGACGGGAGAATTATCGAAG
CGCAGCAGCATAATCAGGCTTGGGACGCTGTTATACAGCTTCTCGAGGAATTTGTGGAAATGATGGGCGAAGACGAAATT
TCTCTGTCTCTTTTTCAGCAAATGATGGAGACGGGTGCGGAATCGTTAAAATTCGCTCTGATTCCGCCTGCACTCGATCA
AGTCTTTGTCGGCAATATGGATTTATCGAGAATGTACGGTACACGCTGCACCTTTGTTATCGGTGCGAACGACGGCGTAC
TTCCGGCGCGGCCTGAGGATGACGGGGTGCTGTCTGATGACGACAGGGAATGGCTGAAAACCATTGGAGTCGAGCTGTCA
TCCGGAGGGCGCGAACGTCTGCTTGATGAGCACTTTCTCATATATATGGCGCTTTCCAGTCCGTCTGACCGCCTTTATGT
ATCCTACCCGATCGCAGACGCCGAAGGTAAAACGCTTTTGCCGTCAACTGTGGTCAAACGGCTTACAGAACTGTTTCCGG
AGCATCAGGAAAGGCTTTTGACCAATGAACCGGAACAGGTCAGCGACTGCGAACAGCTCTTGTATCTCGTCAATAAAAGC
GTGGCGCAATCGTATACGGCAAGCCAATTCAGGTTGTGGTTAAGGGAGTATGACATCAGTGATGTATGGTGGAGCGCGTA
TAATGTATTGATGAATGAACCGGACAGGCTGAACTCGAAAAAACTTTTCTCCAGCCTGTTTTTCAGAAATGAAGTCAAAC
CGCTTGAACGCGCTGTATCGAGACAGCTGTACGGCGAGCATATTCATGGAAGTGTGTCCAGAATGGAGACCTTTAACGCT
TGTCCGTTCTCCCATTTTGCGTCCCACGGCTTACATCTGAAAGAACGGCAATTTTTCAAGCTGGAAGCGCCGGATATCGG
ACAGCTGTTCCATTCCAGTCTGAAGCTGATTTCAGACCGGCTTCGTGAACAAAAGCTGGATTGGCGCGATTTAACAAAAG
ACCAATGTAATAAGTTCTCTTACGAGGCGGTGGAGCGGCTCGCGCCGAAGCTCCAAAAAGAAATTCTGCTTAGCTCAAAC
AGACATTATTATGTCAAGGAAAAGCTGCAAAAAATTGTGGCGCGCGTGTCCGGCATTTTAAGCGAGCATGCGAAGGCGAG
TGGGTTTGCACCGGTTGGGCTTGAGCTTGGTTTTGGCGGGAAAGGCCCGCTGCCGCCTCTTACATTCACATTAAAAAACG
GCTGCACGATGGAGCTTGTCGGACGCATTGACCGCGTAGATAAAGCGGAAAGTTCAAAAGGGCTGCTGCTGAGAATAGTG
GATTATAAATCCAGCGACAAGGGTCTTGATTTGTCAGAGGTATACTACGGCCTTGCCCTGCAAATGCTCACGTACCTTGA
TTTATCCATTACGTATTCGGCAGATTGGCTCGGCATGAAGGCTACACCGGCCGGCGTTCTTTATTTCCATATTCACGACC
CGATGATTCAAGCAAGCGTTCCGCTCGGGCTTGATGAAATAGAGCAGGAAATTTTTAAGAAATTTAAGATGAAAGGCCTG
CTGCTCGGCGATCAGGAAGCGATTCGATTAATGGATACGACGCTCCAAGAGGGAAGGTCAAATATCATTAATGCCGGGCT
TAAAAAAGACGGTTCATTAAGGTCTGATTCAGCGGCGGTCGGAGAAGAGGAATTTCATCTGCTGACACAGCACGTGCGGC
GGACCTTTCAGGAGGCCGGCGAACAAATCACCGACGGTTGTGTGTCGATTGAGCCGTATAAGCTGAAAGATAAAACACCC
TGCACATACTGCTCATTCAAATCAGTATGCCAGTTCGATGAATCATTGGAAGAAAACGAATATCGCACGCTGAAAGCGGA
AAAGGATCATACCATATTAGATTGGCTGAAAAAGGAGGGAGATGGCGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

88.679

100

0.887