Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   INT76_RS04340 Genome accession   NZ_CP073084
Coordinates   857966..859258 (-) Length   430 a.a.
NCBI ID   WP_212572574.1    Uniprot ID   -
Organism   Streptococcus oriscaviae strain HKU75     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 852966..864258
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INT76_RS04320 (INT76_04320) - 855073..855894 (-) 822 WP_212572566.1 pyruvate, water dikinase regulatory protein -
  INT76_RS04325 (INT76_04325) - 855891..856514 (-) 624 WP_212572568.1 helix-turn-helix transcriptional regulator -
  INT76_RS04330 (INT76_04330) raiA 856689..857231 (-) 543 WP_212572570.1 ribosome-associated translation inhibitor RaiA -
  INT76_RS04335 (INT76_04335) - 857308..857973 (-) 666 WP_212572572.1 ComF family protein -
  INT76_RS04340 (INT76_04340) comFA/cflA 857966..859258 (-) 1293 WP_212572574.1 DEAD/DEAH box helicase Machinery gene
  INT76_RS04345 (INT76_04345) - 859352..859984 (+) 633 WP_212572576.1 YigZ family protein -
  INT76_RS04350 (INT76_04350) cysK 860075..860998 (+) 924 WP_212572578.1 cysteine synthase A -
  INT76_RS04355 (INT76_04355) - 861189..862670 (+) 1482 WP_212572580.1 ABC transporter permease -
  INT76_RS04360 (INT76_04360) - 862682..863356 (+) 675 WP_212572582.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48422.73 Da        Isoelectric Point: 7.5754

>NTDB_id=557834 INT76_RS04340 WP_212572574.1 857966..859258(-) (comFA/cflA) [Streptococcus oriscaviae strain HKU75]
MTGFEHLYGRLLTSGQLTEEERQMANRLASKTKDGSCFRCGTAFVAEHSLPNGAVYCRECLMLGRVRTDEDLYYFPQQGF
PKQQSLCWTGQLTDWQQQVSDGLCQAVAERRASLVHAVTGAGKTEMIYQAVASVIDEGGAVCLASPRIDVCIELYKRLSK
DFACPISLLHGESEAYFRTPLVIATTHQLLKFYQAFDLILIDEVDAFPYVDNPMLYRAVENACKPDGVQVFLTATSTEAL
DKKVKRGDLERFRLPRRFHGNPLVPPQKVWLAHFERELGKKKLVKKLERAIRKQRESGYPLLIFVPEIKLGQAFCQVLSQ
VFPEELIGFVSSQTENRLELVEGFRKQELSILVSTTILERGVTFPCVDVFVVDAHHTLYSSSSLVQIAGRVGRSLERPTG
QLLFFHAGSNRAIERAIAEIRLMNKEAGHE

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=557834 INT76_RS04340 WP_212572574.1 857966..859258(-) (comFA/cflA) [Streptococcus oriscaviae strain HKU75]
ATGACAGGATTTGAACACTTATATGGTCGCTTGCTGACCAGCGGTCAGTTGACAGAAGAAGAAAGACAGATGGCTAACCG
ACTAGCCTCAAAAACCAAGGATGGCTCTTGTTTCCGTTGTGGAACGGCCTTTGTGGCGGAGCATAGCCTCCCAAACGGAG
CAGTTTACTGCAGGGAGTGCTTGATGCTTGGCCGGGTGAGGACGGATGAAGACCTTTATTATTTTCCTCAGCAAGGCTTT
CCGAAGCAGCAAAGTCTTTGCTGGACGGGGCAGCTGACTGACTGGCAGCAACAGGTTTCGGATGGTTTGTGTCAGGCGGT
TGCTGAGCGAAGGGCTAGTCTGGTTCACGCGGTTACGGGAGCTGGCAAGACAGAGATGATCTATCAGGCGGTTGCTTCAG
TGATTGATGAGGGAGGGGCGGTTTGTTTGGCTAGCCCACGCATTGATGTGTGCATTGAACTGTATAAGCGATTGAGTAAG
GATTTTGCTTGTCCGATTAGTTTGCTGCATGGAGAGTCAGAGGCTTATTTTCGCACCCCCTTGGTTATTGCGACCACTCA
TCAGCTTCTCAAATTTTATCAGGCTTTTGATTTGATATTGATTGATGAGGTAGATGCCTTTCCTTATGTGGACAATCCCA
TGCTCTATCGGGCGGTGGAGAATGCCTGTAAACCCGATGGGGTTCAGGTCTTTTTGACAGCAACTTCAACAGAAGCCTTG
GATAAAAAAGTCAAGCGTGGGGATTTAGAGCGCTTCCGCCTTCCCAGACGATTCCATGGCAATCCCTTGGTTCCTCCTCA
AAAAGTTTGGTTGGCACATTTTGAAAGGGAGTTAGGCAAAAAGAAATTGGTCAAGAAGTTGGAAAGGGCAATCCGAAAGC
AACGAGAAAGTGGCTATCCTCTGTTGATTTTTGTTCCAGAAATAAAGTTGGGGCAAGCTTTTTGTCAGGTGCTCAGCCAG
GTTTTTCCAGAGGAGTTGATCGGTTTTGTATCTAGTCAGACGGAAAATCGGCTGGAGCTGGTGGAGGGCTTTCGGAAGCA
GGAGCTGTCGATTTTGGTATCAACGACCATCTTGGAGCGTGGGGTGACTTTTCCTTGTGTAGATGTCTTTGTCGTTGATG
CCCACCATACCCTGTATAGTTCTTCTTCACTCGTCCAGATTGCAGGGCGCGTTGGACGGAGCTTGGAGCGGCCAACAGGA
CAGTTGCTCTTCTTTCATGCTGGCAGTAACCGGGCTATCGAACGGGCTATTGCAGAAATTCGCTTGATGAATAAGGAGGC
TGGTCATGAGTGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(286-393)

Type III restriction enzyme, res subunit (ResIII)

(91-235)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

65.485

98.372

0.644
  comFA/cflA Streptococcus mitis SK321

65.248

98.372

0.642
  comFA/cflA Streptococcus pneumoniae R6

65.012

98.372

0.64
  comFA/cflA Streptococcus pneumoniae Rx1

65.012

98.372

0.64
  comFA/cflA Streptococcus pneumoniae D39

65.012

98.372

0.64
  comFA/cflA Streptococcus pneumoniae TIGR4

64.775

98.372

0.637
  comFA Lactococcus lactis subsp. cremoris KW2

52.926

91.395

0.484
  comFA Latilactobacillus sakei subsp. sakei 23K

38.657

100

0.388