Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA5   Type   Machinery gene
Locus tag   KBZ93_RS10450 Genome accession   NZ_CP073017
Coordinates   2219990..2220535 (+) Length   181 a.a.
NCBI ID   WP_223211273.1    Uniprot ID   -
Organism   Synechocystis sp. PCC 6803     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2214990..2225535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBZ93_RS10430 (KBZ93_10440) - 2215055..2215300 (+) 246 WP_020862237.1 hypothetical protein -
  KBZ93_RS10435 (KBZ93_10445) - 2215380..2218079 (-) 2700 WP_010873202.1 chloride channel protein -
  KBZ93_RS10440 (KBZ93_10450) - 2218194..2218697 (+) 504 WP_020862235.1 hypothetical protein -
  KBZ93_RS10445 (KBZ93_10455) - 2218645..2219865 (+) 1221 WP_010873204.1 DUF58 domain-containing protein -
  KBZ93_RS10450 (KBZ93_10460) pilA/pilA5 2219990..2220535 (+) 546 WP_223211273.1 type II secretion system protein J Machinery gene
  KBZ93_RS10455 (KBZ93_10465) - 2220558..2221103 (+) 546 WP_231848034.1 Tfp pilus assembly protein FimT/FimU -
  KBZ93_RS10460 (KBZ93_10470) - 2221220..2221780 (+) 561 WP_010873207.1 Tfp pilus assembly protein FimT/FimU -
  KBZ93_RS10465 (KBZ93_10475) - 2221802..2222719 (+) 918 WP_010873208.1 PilW family protein -
  KBZ93_RS10470 (KBZ93_10480) - 2222777..2223472 (+) 696 WP_010873209.1 alpha/beta fold hydrolase -
  KBZ93_RS10475 (KBZ93_10485) - 2223693..2223983 (-) 291 WP_223211274.1 hypothetical protein -
  KBZ93_RS10480 (KBZ93_10490) - 2224182..2224613 (-) 432 WP_010873211.1 hypothetical protein -
  KBZ93_RS10485 (KBZ93_10495) - 2224670..2225434 (-) 765 WP_010873212.1 IS5 family transposase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 19521.27 Da        Isoelectric Point: 5.2061

>NTDB_id=557388 KBZ93_RS10450 WP_223211273.1 2219990..2220535(+) (pilA/pilA5) [Synechocystis sp. PCC 6803]
MFKAIFPRYSVGDRRLLESVRHSAQQGFTMFEVLIALMISFLFLTGTLNAMVMATVMRVKAEREAQAGYWIKEDLEQVRA
LAANFNPATGNGCPTSVGETFRDANNSADADPNNDGLASLPVPANKSIVGVTAAITRTPGVSNNVLTLSYRVRNTNVTDN
DMATLATLYTEVLPAAALACE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=557388 KBZ93_RS10450 WP_223211273.1 2219990..2220535(+) (pilA/pilA5) [Synechocystis sp. PCC 6803]
ATGTTTAAGGCTATTTTCCCAAGGTATTCCGTTGGCGATCGCCGTTTGCTAGAGTCAGTCCGCCACTCAGCTCAGCAGGG
TTTCACCATGTTCGAGGTGCTGATTGCCTTGATGATTTCCTTTCTATTTCTGACAGGAACCCTGAATGCCATGGTAATGG
CAACGGTAATGCGGGTTAAAGCGGAAAGAGAGGCCCAAGCTGGCTATTGGATAAAAGAAGATTTAGAGCAAGTTCGGGCA
TTAGCTGCAAACTTCAATCCGGCAACTGGTAATGGATGCCCTACAAGTGTGGGTGAAACTTTTCGAGATGCAAACAATAG
TGCTGACGCCGATCCTAACAATGATGGACTAGCGAGCTTACCAGTTCCTGCCAACAAATCAATTGTCGGTGTAACAGCCG
CTATTACTAGAACACCAGGGGTATCTAATAACGTTTTAACTCTTAGCTATCGTGTCAGGAACACCAATGTCACCGACAAT
GATATGGCTACCCTTGCAACACTTTACACCGAGGTTCTTCCTGCTGCCGCTCTTGCTTGTGAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(24-47)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA5 Synechocystis sp. PCC 6803

100

83.978

0.84