Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   J8L76_RS04350 Genome accession   NZ_CP072875
Coordinates   945161..946663 (+) Length   500 a.a.
NCBI ID   WP_216130396.1    Uniprot ID   A0A975XTH0
Organism   Azospira inquinata strain Azo-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 940161..951663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8L76_RS04325 (J8L76_04325) bfr 940657..941145 (+) 489 WP_216130386.1 bacterioferritin -
  J8L76_RS04330 (J8L76_04330) - 941191..942747 (-) 1557 WP_216130388.1 PAS domain-containing protein -
  J8L76_RS04335 (J8L76_04335) recA 942859..943893 (+) 1035 WP_216130390.1 recombinase RecA Machinery gene
  J8L76_RS04340 (J8L76_04340) recX 943886..944326 (+) 441 WP_216130392.1 recombination regulator RecX -
  J8L76_RS04345 (J8L76_04345) lptB 944339..945088 (+) 750 WP_216130394.1 LPS export ABC transporter ATP-binding protein -
  J8L76_RS04350 (J8L76_04350) rpoN/rpoN1 945161..946663 (+) 1503 WP_216130396.1 RNA polymerase factor sigma-54 Machinery gene
  J8L76_RS04355 (J8L76_04355) hpf 946680..947003 (+) 324 WP_216130398.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  J8L76_RS04360 (J8L76_04360) ptsN 947155..947640 (+) 486 WP_216130400.1 PTS IIA-like nitrogen regulatory protein PtsN -
  J8L76_RS04365 (J8L76_04365) hprK 947609..948547 (+) 939 WP_216130402.1 HPr(Ser) kinase/phosphatase -
  J8L76_RS04370 (J8L76_04370) - 948623..948976 (+) 354 WP_216130404.1 PsiF family protein -
  J8L76_RS04375 (J8L76_04375) - 949092..949988 (-) 897 WP_216130406.1 adenylate/guanylate cyclase domain-containing protein -
  J8L76_RS04380 (J8L76_04380) ttcA 950116..951021 (-) 906 WP_216130408.1 tRNA 2-thiocytidine(32) synthetase TtcA -

Sequence

Protein


Download         Length: 500 a.a.        Molecular weight: 55576.53 Da        Isoelectric Point: 4.7134

>NTDB_id=556484 J8L76_RS04350 WP_216130396.1 945161..946663(+) (rpoN/rpoN1) [Azospira inquinata strain Azo-2]
MKPTLQLKLSQHLALTPQLQQSIRLLQLSTLELNQELEKYLTENPLLEREDEDFDGPSGPAAGEGSDSAAPDPAGTTPRE
TERGEDDSREHGEPGESAETGIRASDEGNALAAGEDDGWYAEGSYPSASRDEEDDSDYQDIQAASTSLRDHLAWQLGLTP
LSDRDRNLVRFLIEALDDDGYLTQPLEELADALPPELEIDLDDLLIALRHLQHFDPTGVGARSPQECLALQLEALPPEPT
RDLALQLVRQYLELLAGRDFTKLKKLLGCDDDQLRAAAHLVKSLNPRPGAQYAPLDTRYIIPDVVVRKLRNQWVASINPD
AYPRLRINRLYSDILSKHRGSSLAGQLQEARWLIKNVQQRFDTILRVSQAIVERQRQFFEHGEVAMRPLVLREIAEVLGL
HESTVSRVTTQKYMATPRGIFELKYFFGSHVATEAGGACSATAIRALIKQLIGGEDAKKPLSDSQISEILGQQGIVVARR
TIAKYREALNIPPANLRKTI

Nucleotide


Download         Length: 1503 bp        

>NTDB_id=556484 J8L76_RS04350 WP_216130396.1 945161..946663(+) (rpoN/rpoN1) [Azospira inquinata strain Azo-2]
ATGAAACCGACCCTCCAACTTAAACTTTCCCAGCATTTGGCGCTCACGCCCCAACTGCAACAATCCATCCGGTTGTTGCA
GCTGTCCACGCTGGAACTGAACCAGGAGCTGGAGAAGTACCTGACGGAAAATCCCCTCCTGGAACGGGAGGACGAGGATT
TCGACGGTCCTTCCGGGCCTGCCGCCGGGGAAGGAAGTGACAGTGCGGCCCCTGATCCGGCGGGCACCACGCCCCGGGAA
ACGGAGCGGGGCGAGGATGATAGCCGGGAGCACGGGGAGCCGGGGGAAAGCGCCGAAACGGGCATCCGTGCCAGCGACGA
GGGCAATGCCCTGGCCGCCGGGGAGGATGACGGCTGGTATGCGGAGGGCAGCTATCCTTCCGCCTCCCGGGACGAAGAAG
ACGATTCGGATTACCAGGACATCCAGGCCGCCAGCACCTCCCTGCGAGACCATCTGGCCTGGCAGTTAGGCCTCACCCCC
CTGAGTGACCGGGACCGGAATCTGGTACGCTTTCTCATTGAAGCCCTGGACGACGACGGCTACCTGACCCAGCCCCTGGA
AGAACTGGCGGACGCCCTGCCCCCGGAGCTCGAAATCGACCTGGACGACCTGCTCATCGCCCTGCGCCACCTCCAGCATT
TCGACCCCACCGGAGTGGGGGCCCGCTCTCCCCAGGAATGCCTGGCCCTGCAACTGGAAGCCCTGCCCCCGGAACCCACC
CGGGATCTGGCCCTGCAACTGGTGCGCCAGTATCTGGAACTCCTGGCCGGACGGGACTTCACCAAGCTGAAAAAGCTCCT
GGGCTGTGACGACGACCAGCTACGGGCCGCCGCCCATCTGGTGAAGAGCCTCAATCCCCGCCCCGGGGCCCAGTACGCCC
CCCTGGATACCCGCTACATTATTCCCGACGTGGTGGTGCGCAAGCTACGCAACCAGTGGGTGGCCAGCATCAACCCGGAC
GCCTATCCCCGGCTGCGCATCAACCGCCTGTATTCGGATATTCTCTCCAAACACCGGGGCTCCTCCCTGGCTGGCCAGCT
CCAAGAAGCCCGCTGGCTGATCAAGAACGTACAGCAGCGCTTTGACACCATTCTGCGGGTCTCCCAGGCCATCGTGGAAC
GCCAGCGCCAGTTCTTCGAACACGGGGAAGTGGCCATGCGCCCCCTGGTACTGCGGGAAATCGCCGAGGTGCTGGGGCTC
CACGAATCCACCGTTTCCCGGGTCACCACCCAGAAATACATGGCCACCCCCCGGGGCATTTTCGAACTCAAGTATTTCTT
CGGCAGCCACGTGGCCACGGAAGCGGGGGGCGCCTGTTCCGCCACCGCCATCCGGGCCCTGATCAAGCAGTTGATTGGCG
GGGAAGATGCGAAAAAGCCCCTTTCCGACAGCCAGATTTCCGAGATTCTCGGGCAACAGGGCATTGTGGTGGCCCGCCGG
ACCATAGCAAAATACCGGGAGGCGTTGAACATCCCGCCGGCTAACCTACGCAAGACCATCTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-331)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-498)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

57.171

100

0.582