Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   J9319_RS11650 Genome accession   NZ_CP072851
Coordinates   2250146..2251216 (-) Length   356 a.a.
NCBI ID   WP_017357987.1    Uniprot ID   -
Organism   Bacillus altitudinis strain 6ww6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2245146..2256216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9319_RS11600 (J9319_11600) - 2245242..2245814 (-) 573 WP_017357979.1 signal peptidase I -
  J9319_RS11605 (J9319_11605) tapA 2245864..2246391 (-) 528 WP_116307132.1 amyloid fiber anchoring/assembly protein TapA -
  J9319_RS11610 (J9319_11610) - 2246644..2246970 (+) 327 WP_083561443.1 DUF3889 domain-containing protein -
  J9319_RS11615 (J9319_11615) - 2247004..2247198 (-) 195 WP_034319624.1 YqzE family protein -
  J9319_RS11620 (J9319_11620) comGG 2247259..2247642 (-) 384 WP_116307134.1 competence type IV pilus minor pilin ComGG -
  J9319_RS11625 (J9319_11625) comGF 2247639..2248136 (-) 498 WP_116307136.1 competence type IV pilus minor pilin ComGF -
  J9319_RS11630 (J9319_11630) comGE 2248078..2248392 (-) 315 WP_081114339.1 competence type IV pilus minor pilin ComGE -
  J9319_RS11635 (J9319_11635) comGD 2248376..2248822 (-) 447 WP_116307138.1 competence type IV pilus minor pilin ComGD -
  J9319_RS11640 (J9319_11640) comGC 2248815..2249108 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  J9319_RS11645 (J9319_11645) comGB 2249125..2250165 (-) 1041 WP_116307140.1 competence type IV pilus assembly protein ComGB -
  J9319_RS11650 (J9319_11650) comGA 2250146..2251216 (-) 1071 WP_017357987.1 competence type IV pilus ATPase ComGA Machinery gene
  J9319_RS11655 (J9319_11655) - 2251682..2252554 (-) 873 WP_116307142.1 STAS domain-containing protein -
  J9319_RS11665 (J9319_11665) - 2252780..2253160 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  J9319_RS11670 (J9319_11670) - 2253373..2253615 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  J9319_RS11675 (J9319_11675) - 2253664..2254302 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  J9319_RS11680 (J9319_11680) - 2254463..2254636 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  J9319_RS11685 (J9319_11685) - 2254661..2254975 (-) 315 WP_008342314.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40339.70 Da        Isoelectric Point: 9.0449

>NTDB_id=556381 J9319_RS11650 WP_017357987.1 2250146..2251216(-) (comGA) [Bacillus altitudinis strain 6ww6]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=556381 J9319_RS11650 WP_017357987.1 2250146..2251216(-) (comGA) [Bacillus altitudinis strain 6ww6]
TTGTATGGTATTGAATATTTGGGACAAGAGCTGCTAGAAGAGGCATGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGGTCATTGGCT
GTGATGCTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCGACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACTGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGAAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACACTACAT
GCAAAAAATGCAAAAGGAGCTCTTTATCGAATGCTGGAATTTGGAGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGATGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

65.254

99.438

0.649