Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   J9174_RS07100 Genome accession   NZ_CP072837
Coordinates   1366946..1367917 (-) Length   323 a.a.
NCBI ID   WP_164942281.1    Uniprot ID   -
Organism   Macrococcoides canis strain 19/EPI0118     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1361946..1372917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9174_RS07065 (J9174_07065) gcvT 1362641..1363726 (-) 1086 WP_210153031.1 glycine cleavage system aminomethyltransferase GcvT -
  J9174_RS07070 (J9174_07070) - 1363864..1364370 (-) 507 WP_164942276.1 shikimate kinase -
  J9174_RS07075 (J9174_07075) - 1364531..1364983 (-) 453 WP_210151817.1 competence type IV pilus minor pilin ComGF -
  J9174_RS07080 (J9174_07080) - 1364928..1365170 (-) 243 WP_158290712.1 hypothetical protein -
  J9174_RS07085 (J9174_07085) comGD 1365157..1365582 (-) 426 WP_164953585.1 competence type IV pilus minor pilin ComGD -
  J9174_RS07090 (J9174_07090) comGC 1365575..1365907 (-) 333 WP_174804829.1 competence type IV pilus major pilin ComGC -
  J9174_RS07095 (J9174_07095) comGB 1365922..1366971 (-) 1050 WP_210151818.1 competence type IV pilus assembly protein ComGB -
  J9174_RS07100 (J9174_07100) comGA 1366946..1367917 (-) 972 WP_164942281.1 competence type IV pilus ATPase ComGA Machinery gene
  J9174_RS07105 (J9174_07105) - 1368130..1368378 (+) 249 WP_086042733.1 DUF2626 domain-containing protein -
  J9174_RS07110 (J9174_07110) - 1368407..1369027 (-) 621 WP_210151819.1 MBL fold metallo-hydrolase -
  J9174_RS07115 (J9174_07115) - 1369117..1369356 (+) 240 WP_174804788.1 DUF2759 domain-containing protein -
  J9174_RS07120 (J9174_07120) - 1369383..1369694 (-) 312 WP_086042736.1 MTH1187 family thiamine-binding protein -
  J9174_RS07125 (J9174_07125) - 1369694..1370662 (-) 969 WP_210151820.1 ROK family glucokinase -
  J9174_RS07130 (J9174_07130) - 1370659..1370850 (-) 192 WP_086042738.1 YqgQ family protein -
  J9174_RS07135 (J9174_07135) - 1370855..1372303 (-) 1449 WP_210151821.1 rhomboid family protein -
  J9174_RS07140 (J9174_07140) - 1372279..1372833 (-) 555 WP_210151822.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 37275.84 Da        Isoelectric Point: 6.5751

>NTDB_id=556035 J9174_RS07100 WP_164942281.1 1366946..1367917(-) (comGA) [Macrococcoides canis strain 19/EPI0118]
MEQLFNEIIEEAIIQSASDIHFIPNDKSISIKFRIHGDIEDYREIDDMLFRKLLSYIKFTAHLDVSEKNKAQSGIIQFNL
KDLRYNIRASTLPRSLGDEACVLRIIRQNFIDEYQTNDKILYNQIKKSSGIIIISGPTGSGKSTLMYQLVHYARNTLKRQ
IISIEDPVEQHIEGIIQVNVNEKAEITYHTAIKAILRCDPDIIMLGEIRDSVVAHQVINAGLSGHLVLTTLHANDCIGAL
FRLKEMGINAVDLYQSINLIINQRLVRKKDGMGRLLAYEFLTKKDIEKYLQGEHINYRTLTDQFKELYETNQISHYEFEK
FDL

Nucleotide


Download         Length: 972 bp        

>NTDB_id=556035 J9174_RS07100 WP_164942281.1 1366946..1367917(-) (comGA) [Macrococcoides canis strain 19/EPI0118]
ATGGAACAGTTATTCAATGAAATAATAGAGGAAGCAATTATACAAAGTGCATCAGATATTCACTTCATACCAAACGATAA
AAGCATATCTATCAAATTTAGGATACATGGTGATATTGAAGACTATAGAGAAATCGATGACATGTTATTCAGGAAATTAC
TTTCTTATATTAAATTTACAGCACATCTTGATGTATCAGAAAAGAATAAGGCACAGAGTGGAATCATCCAATTTAATCTG
AAAGACTTAAGATATAATATTCGCGCATCTACTTTACCTCGATCATTAGGCGATGAAGCATGTGTACTGAGAATCATCAG
ACAAAATTTTATAGATGAATATCAGACGAATGACAAGATATTGTACAATCAAATAAAAAAATCAAGCGGTATTATCATCA
TAAGTGGACCAACTGGAAGCGGGAAAAGCACATTGATGTATCAGCTTGTACATTATGCGAGGAACACATTGAAGAGACAA
ATAATATCAATAGAAGATCCTGTTGAACAGCATATTGAAGGAATTATACAGGTTAATGTTAACGAAAAAGCAGAGATAAC
GTATCATACTGCGATAAAGGCAATCCTTAGATGTGACCCGGATATAATTATGCTAGGTGAAATCAGAGATTCAGTCGTAG
CACATCAAGTGATCAATGCAGGACTAAGCGGTCATCTCGTTTTAACGACATTACATGCGAATGATTGCATCGGTGCATTA
TTTCGGTTAAAAGAAATGGGTATTAATGCTGTTGATCTATATCAAAGTATCAATCTGATTATTAATCAAAGGCTTGTAAG
AAAAAAAGATGGGATGGGGCGTCTATTGGCCTATGAATTTTTAACAAAAAAGGATATTGAAAAATATTTACAAGGTGAAC
ATATTAACTATAGAACGTTGACCGATCAATTTAAGGAGCTCTATGAAACAAATCAGATTTCACACTATGAATTCGAAAAA
TTTGATCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

49.39

100

0.502
  comGA Staphylococcus aureus N315

49.39

100

0.502
  comGA Bacillus subtilis subsp. subtilis str. 168

37.73

100

0.381
  pilB Glaesserella parasuis strain SC1401

34.302

100

0.365
  pilB Vibrio parahaemolyticus RIMD 2210633

35.03

100

0.362