Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   J5X96_RS03660 Genome accession   NZ_CP072548
Coordinates   711770..712324 (-) Length   184 a.a.
NCBI ID   WP_209364413.1    Uniprot ID   -
Organism   Aggregatibacter sp. 2125159857     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 706770..717324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5X96_RS03635 (J5X96_03635) - 707033..707485 (-) 453 WP_209364409.1 isoprenylcysteine carboxylmethyltransferase family protein -
  J5X96_RS03640 (J5X96_03640) - 707536..708150 (-) 615 WP_021615980.1 YfgM family protein -
  J5X96_RS03645 (J5X96_03645) hisS 708160..709437 (-) 1278 WP_209364410.1 histidine--tRNA ligase -
  J5X96_RS03650 (J5X96_03650) ispG 709463..710566 (-) 1104 WP_209364411.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  J5X96_RS03655 (J5X96_03655) - 710577..711554 (-) 978 WP_209364412.1 RodZ family helix-turn-helix domain-containing protein -
  J5X96_RS03660 (J5X96_03660) pilF/pilF2 711770..712324 (-) 555 WP_209364413.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  J5X96_RS03665 (J5X96_03665) - 712350..713504 (-) 1155 WP_209364414.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  J5X96_RS03670 (J5X96_03670) epmA 713672..714643 (-) 972 WP_209364415.1 elongation factor P--(R)-beta-lysine ligase -
  J5X96_RS03675 (J5X96_03675) frdA 714968..716770 (+) 1803 WP_209364416.1 fumarate reductase (quinol) flavoprotein subunit -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20944.75 Da        Isoelectric Point: 7.7737

>NTDB_id=554094 J5X96_RS03660 WP_209364413.1 711770..712324(-) (pilF/pilF2) [Aggregatibacter sp. 2125159857]
MRPIQFIFYRFAIFIFPFLLSACVSHSDTSDFDKQAAAKARVELALGYLQQQNGSQAKLNLDKALSYAPKYYLVHAALAY
FYQQQGDMEHAKQAYLTAIKLDDTQGDVLNNFGAFLCAQGEYQAAYKQFTQALNSPQYYHQADTYENLALCALSAKDQKI
YQENLTALEKIAPERAKRFAQFTK

Nucleotide


Download         Length: 555 bp        

>NTDB_id=554094 J5X96_RS03660 WP_209364413.1 711770..712324(-) (pilF/pilF2) [Aggregatibacter sp. 2125159857]
ATGCGCCCTATTCAGTTTATCTTTTACCGTTTTGCGATCTTTATTTTTCCTTTTTTACTTTCTGCCTGCGTCAGCCATTC
CGACACATCAGATTTTGATAAACAGGCGGCGGCAAAAGCCCGTGTAGAACTGGCATTAGGTTATTTACAACAACAAAACG
GTAGCCAAGCGAAATTAAATTTAGATAAAGCCCTTTCTTATGCCCCAAAATACTATTTGGTACACGCTGCGTTAGCCTAT
TTTTATCAACAACAAGGGGATATGGAACACGCTAAACAAGCGTATTTAACGGCCATTAAATTGGATGATACACAAGGGGA
TGTCTTGAATAATTTCGGTGCGTTTTTGTGTGCGCAAGGAGAATATCAGGCGGCCTATAAACAATTCACACAGGCATTAA
ATAGCCCGCAGTATTATCACCAAGCCGACACTTATGAAAACCTGGCACTCTGCGCCTTATCCGCGAAAGATCAGAAAATT
TACCAAGAAAATCTGACCGCACTTGAGAAGATCGCACCAGAACGTGCAAAGAGATTCGCTCAATTCACAAAATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(75-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

55.114

95.652

0.527
  pilF Glaesserella parasuis strain SC1401

47.399

94.022

0.446