Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   J5A60_RS07380 Genome accession   NZ_CP072381
Coordinates   1538861..1539412 (-) Length   183 a.a.
NCBI ID   WP_212509945.1    Uniprot ID   -
Organism   Aggregatibacter sp. oral taxon 513 strain W11186     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1533861..1544412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5A60_RS07355 (J5A60_07355) - 1534057..1534509 (-) 453 WP_212509944.1 isoprenylcysteine carboxylmethyltransferase family protein -
  J5A60_RS07360 (J5A60_07360) - 1534564..1535178 (-) 615 WP_048749277.1 YfgM family protein -
  J5A60_RS07365 (J5A60_07365) hisS 1535188..1536465 (-) 1278 WP_006719281.1 histidine--tRNA ligase -
  J5A60_RS07370 (J5A60_07370) ispG 1536491..1537594 (-) 1104 WP_021615982.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  J5A60_RS07375 (J5A60_07375) - 1537605..1538645 (-) 1041 WP_109104055.1 RodZ family helix-turn-helix domain-containing protein -
  J5A60_RS07380 (J5A60_07380) pilF/pilF2 1538861..1539412 (-) 552 WP_212509945.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  J5A60_RS07385 (J5A60_07385) - 1539437..1540591 (-) 1155 WP_006719285.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  J5A60_RS07390 (J5A60_07390) - 1540831..1541349 (-) 519 WP_109104057.1 SprT family zinc-dependent metalloprotease -
  J5A60_RS07395 (J5A60_07395) - 1541561..1544368 (+) 2808 WP_109104058.1 autotransporter outer membrane beta-barrel domain-containing protein -

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 21087.10 Da        Isoelectric Point: 8.5794

>NTDB_id=552252 J5A60_RS07380 WP_212509945.1 1538861..1539412(-) (pilF/pilF2) [Aggregatibacter sp. oral taxon 513 strain W11186]
MVHFSFFTRIIPFIFPFLFSACVSQQVSSDFDKQQAAKARVELALGYLQQRDYNQAKANLDRALSYAPDDYLVHAVRAYF
YQQQGDVIQAEKAYLTAIKLDKKQGDVFNNFGTFLCLQGKYSAAYEQFHKALKAPNYYRQTDTYENLALCALSAKDLTVY
QENLLMLEKMDPERAKKLALRAQ

Nucleotide


Download         Length: 552 bp        

>NTDB_id=552252 J5A60_RS07380 WP_212509945.1 1538861..1539412(-) (pilF/pilF2) [Aggregatibacter sp. oral taxon 513 strain W11186]
ATGGTTCATTTCTCGTTCTTTACTCGAATCATACCGTTTATTTTCCCCTTTCTGTTTTCTGCCTGTGTTTCCCAACAGGT
ATCATCTGATTTTGATAAACAACAGGCGGCAAAAGCTCGTGTTGAATTAGCGCTGGGCTATTTGCAGCAACGAGATTACA
ATCAAGCCAAGGCAAATTTAGATCGCGCGCTTTCTTATGCGCCCGATGACTATTTAGTGCATGCGGTGCGCGCTTATTTT
TATCAGCAACAGGGCGATGTGATTCAAGCAGAGAAAGCCTATTTGACTGCTATTAAGTTAGACAAAAAGCAAGGTGATGT
TTTCAATAATTTCGGTACCTTTCTTTGTTTGCAAGGTAAATATTCTGCCGCCTATGAGCAATTCCATAAAGCATTAAAGG
CGCCTAATTATTATCGGCAGACTGATACTTACGAAAATCTTGCACTCTGTGCTTTATCAGCAAAGGATTTAACGGTTTAT
CAGGAAAATCTGTTAATGCTTGAAAAAATGGATCCGGAAAGAGCAAAAAAATTAGCTTTGCGGGCACAATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_19)

(48-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

55.488

89.617

0.497
  pilF Glaesserella parasuis strain SC1401

44.624

100

0.454