Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   J4854_RS02695 Genome accession   NZ_CP072329
Coordinates   517961..519259 (+) Length   432 a.a.
NCBI ID   WP_200772965.1    Uniprot ID   A0A9X0WNU5
Organism   Streptococcus lactarius strain CCUG 66490     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 519999..534789 517961..519259 flank 740


Gene organization within MGE regions


Location: 517961..534789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4854_RS02695 (J4854_02695) comFA/cflA 517961..519259 (+) 1299 WP_200772965.1 DEAD/DEAH box helicase Machinery gene
  J4854_RS02700 (J4854_02700) - 519256..519921 (+) 666 WP_200772964.1 ComF family protein -
  J4854_RS02705 (J4854_02705) hpf 519999..520541 (+) 543 WP_200772963.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  J4854_RS02780 (J4854_02780) - 526819..527352 (-) 534 WP_187540768.1 DUF402 domain-containing protein -
  J4854_RS02785 (J4854_02785) recX 527462..528244 (-) 783 WP_200772148.1 recombination regulator RecX -
  J4854_RS02790 (J4854_02790) - 528255..529031 (-) 777 WP_200772149.1 aminoglycoside 3'-phosphotransferase -
  J4854_RS02795 (J4854_02795) rlmD 529087..530439 (+) 1353 WP_200772150.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
  J4854_RS02800 (J4854_02800) - 530472..530705 (+) 234 WP_200772151.1 DUF1858 domain-containing protein -
  J4854_RS02805 (J4854_02805) - 530705..532051 (+) 1347 WP_200772152.1 DUF438 domain-containing protein -
  J4854_RS02810 (J4854_02810) - 532054..532308 (+) 255 WP_013903179.1 DUF1912 family protein -
  J4854_RS02815 (J4854_02815) - 532489..532836 (+) 348 WP_200772153.1 helix-hairpin-helix domain-containing protein -
  J4854_RS02820 (J4854_02820) - 533165..533743 (+) 579 WP_200772154.1 flavin reductase family protein -
  J4854_RS02825 (J4854_02825) - 533730..534290 (+) 561 WP_200772155.1 GNAT family N-acetyltransferase -
  J4854_RS02830 (J4854_02830) - 534319..534789 (+) 471 WP_200772156.1 hypothetical protein -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49224.12 Da        Isoelectric Point: 9.3833

>NTDB_id=552037 J4854_RS02695 WP_200772965.1 517961..519259(+) (comFA/cflA) [Streptococcus lactarius strain CCUG 66490]
MKIEDTYGRLLTKSQMTDDLYKIAQEIPAIEKQNGANVCFRCGSVIDQKLWRLSEDVLYCRACIQLGRLRSDQKLYAIAQ
QDFEGQEVLNWKGTLTSYQQEVSDGLIQAVKGNKNALVHAVTGAGKTEMMYQVVANSIKNGKAVCIATPRIDVCIELYRR
MKEDFSCPISLLHGESEPYFRTPLVIATTHQLLKFYKAFDLLIIDEVDAFPYVDNPVLYKAAQNAIKKEGNTLYLTATST
DELDKKVKKKEVSRYSLPRRFHGNPLVVPEIKWVSKIRQKIEKGKIPHDLLQLIKQQRQTTYPLLLFVSEIELGKQLKDV
LTKYFPKEKLGFVSSKTEDRLRIVEEFRNREVSILVSTTILERGVTFPLVDVFVIESNHCLFTKSTLVQISGRVGRSKER
PTGQLLFLADGNTKAMKNAVKEIKEMNQEAGF

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=552037 J4854_RS02695 WP_200772965.1 517961..519259(+) (comFA/cflA) [Streptococcus lactarius strain CCUG 66490]
ATGAAGATTGAAGATACTTATGGAAGGCTCTTAACCAAGAGTCAAATGACAGATGATTTATATAAAATAGCCCAAGAAAT
TCCAGCAATAGAAAAACAAAATGGGGCCAACGTTTGTTTTAGATGTGGTAGTGTGATTGATCAAAAACTATGGAGGTTGA
GTGAAGATGTATTGTATTGTAGAGCCTGTATCCAATTGGGAAGACTCCGGAGTGATCAGAAGCTGTATGCCATAGCGCAG
CAAGACTTTGAAGGCCAAGAGGTATTGAACTGGAAAGGAACCTTAACTTCATATCAACAAGAGGTATCTGATGGACTGAT
CCAAGCAGTGAAAGGAAACAAGAATGCTTTGGTCCACGCTGTGACAGGTGCCGGAAAAACGGAAATGATGTACCAAGTTG
TAGCGAATTCGATCAAGAATGGTAAAGCAGTTTGTATTGCAACACCACGAATTGATGTTTGTATCGAATTGTACCGAAGA
ATGAAGGAAGATTTTTCCTGCCCCATATCCTTGCTTCATGGAGAGTCGGAGCCTTATTTTAGAACGCCTTTGGTGATCGC
GACCACACACCAATTGTTAAAGTTTTACAAAGCATTTGATCTTCTGATCATTGATGAAGTGGATGCCTTCCCCTATGTGG
ACAATCCCGTGTTATATAAAGCAGCCCAAAATGCAATTAAAAAAGAAGGCAACACCCTTTATTTAACTGCAACCTCTACA
GATGAATTGGACAAGAAAGTAAAGAAAAAGGAAGTCAGTCGTTATAGTCTACCTAGAAGATTTCACGGCAACCCCTTAGT
AGTTCCTGAGATAAAATGGGTTTCAAAAATTAGACAGAAGATAGAAAAAGGAAAAATTCCACATGATTTACTGCAGCTTA
TAAAACAGCAAAGACAAACAACCTATCCCTTGCTACTATTTGTATCAGAAATTGAATTAGGTAAACAATTGAAAGATGTT
TTAACGAAATATTTTCCAAAGGAAAAACTTGGATTTGTTTCTTCTAAAACAGAGGACCGATTACGGATCGTTGAGGAGTT
TAGAAATAGAGAAGTATCCATTTTGGTTTCAACCACCATTTTAGAGAGAGGAGTTACCTTTCCTTTAGTAGACGTCTTTG
TTATAGAAAGTAATCATTGCTTATTTACGAAAAGTACATTGGTACAAATCTCAGGAAGAGTTGGTCGTAGTAAAGAAAGA
CCAACAGGACAGTTACTCTTTTTAGCTGATGGAAACACAAAAGCCATGAAAAACGCTGTAAAGGAAATTAAAGAAATGAA
TCAGGAGGCAGGCTTTTGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(288-397)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis SK321

59.353

100

0.595
  comFA/cflA Streptococcus pneumoniae Rx1

59.122

100

0.593
  comFA/cflA Streptococcus pneumoniae D39

59.122

100

0.593
  comFA/cflA Streptococcus pneumoniae R6

59.122

100

0.593
  comFA/cflA Streptococcus mitis NCTC 12261

59.122

100

0.593
  comFA/cflA Streptococcus pneumoniae TIGR4

58.891

100

0.59
  comFA Lactococcus lactis subsp. cremoris KW2

50.49

94.444

0.477
  comFA Latilactobacillus sakei subsp. sakei 23K

37.558

100

0.377