Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   J4774_RS12325 Genome accession   NZ_CP072310
Coordinates   2469196..2469633 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain AD8     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2464196..2474633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4774_RS19255 - 2464776..2464901 (-) 126 Protein_2395 hypothetical protein -
  J4774_RS12290 - 2465054..2466256 (+) 1203 WP_139890341.1 GTP-binding protein -
  J4774_RS12295 - 2466321..2467457 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  J4774_RS12300 - 2467472..2467906 (+) 435 WP_003156592.1 RDD family protein -
  J4774_RS12305 - 2467978..2468298 (+) 321 WP_014304315.1 YckD family protein -
  J4774_RS12315 - 2468517..2468735 (+) 219 Protein_2400 family 1 glycosylhydrolase -
  J4774_RS12320 nin/comJ 2468776..2469174 (-) 399 WP_014304317.1 competence protein ComJ Regulator
  J4774_RS12325 nucA/comI 2469196..2469633 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  J4774_RS12330 hxlB 2469992..2470549 (-) 558 WP_003156587.1 6-phospho-3-hexuloisomerase -
  J4774_RS12335 hxlA 2470546..2471181 (-) 636 WP_003156586.1 3-hexulose-6-phosphate synthase -
  J4774_RS12340 - 2471413..2471775 (+) 363 WP_003156585.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=551829 J4774_RS12325 WP_003156588.1 2469196..2469633(-) (nucA/comI) [Bacillus velezensis strain AD8]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=551829 J4774_RS12325 WP_003156588.1 2469196..2469633(-) (nucA/comI) [Bacillus velezensis strain AD8]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGTCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641