Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   J4774_RS03060 Genome accession   NZ_CP072310
Coordinates   683424..684494 (-) Length   356 a.a.
NCBI ID   WP_139890048.1    Uniprot ID   -
Organism   Bacillus velezensis strain AD8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 678424..689494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4774_RS03010 - 678483..679067 (-) 585 WP_109567595.1 signal peptidase I -
  J4774_RS03015 tapA 679039..679710 (-) 672 WP_015388007.1 amyloid fiber anchoring/assembly protein TapA -
  J4774_RS03020 - 679970..680299 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  J4774_RS03025 - 680339..680518 (-) 180 WP_003153093.1 YqzE family protein -
  J4774_RS03030 comGG 680575..680952 (-) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  J4774_RS03035 comGF 680953..681348 (-) 396 WP_046559876.1 competence type IV pilus minor pilin ComGF -
  J4774_RS03040 comGE 681362..681676 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  J4774_RS03045 comGD 681660..682097 (-) 438 WP_043867285.1 competence type IV pilus minor pilin ComGD Machinery gene
  J4774_RS03050 comGC 682087..682353 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  J4774_RS03055 comGB 682400..683437 (-) 1038 WP_139890049.1 competence type IV pilus assembly protein ComGB Machinery gene
  J4774_RS03060 comGA 683424..684494 (-) 1071 WP_139890048.1 competence type IV pilus ATPase ComGA Machinery gene
  J4774_RS03065 - 684686..685636 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  J4774_RS03070 - 685782..687083 (+) 1302 WP_014305416.1 hemolysin family protein -
  J4774_RS03075 - 687121..687951 (-) 831 WP_003153080.1 STAS domain-containing protein -
  J4774_RS03085 - 688157..688537 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  J4774_RS03090 - 688774..689019 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40488.84 Da        Isoelectric Point: 9.4677

>NTDB_id=551792 J4774_RS03060 WP_139890048.1 683424..684494(-) (comGA) [Bacillus velezensis strain AD8]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVTMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRKLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=551792 J4774_RS03060 WP_139890048.1 683424..684494(-) (comGA) [Bacillus velezensis strain AD8]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGTTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGAGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCGGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGACCATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAAGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

81.742

100

0.817
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.123

95.787

0.365