Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   DACSA_RS06005 Genome accession   NC_019780
Coordinates   1191377..1193395 (-) Length   672 a.a.
NCBI ID   WP_015228894.1    Uniprot ID   K9YU62
Organism   Dactylococcopsis salina PCC 8305     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1186377..1198395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DACSA_RS05990 (Dacsa_1180) - 1188050..1188619 (-) 570 WP_041235716.1 hypothetical protein -
  DACSA_RS05995 (Dacsa_1181) - 1188639..1189859 (-) 1221 WP_015228891.1 type II secretion system F family protein -
  DACSA_RS06000 (Dacsa_1182) pilT 1189889..1191004 (-) 1116 WP_015228892.1 type IV pilus twitching motility protein PilT Machinery gene
  DACSA_RS06005 (Dacsa_1184) pilB/pilB1 1191377..1193395 (-) 2019 WP_015228894.1 GspE/PulE family protein Machinery gene
  DACSA_RS06010 (Dacsa_1185) bchL 1193991..1194863 (+) 873 WP_015228895.1 ferredoxin:protochlorophyllide reductase (ATP-dependent) iron-sulfur ATP-binding protein -
  DACSA_RS06015 (Dacsa_1186) - 1195025..1196434 (+) 1410 WP_015228896.1 ferredoxin:protochlorophyllide reductase (ATP-dependent) subunit N -
  DACSA_RS06020 (Dacsa_1187) - 1196619..1197851 (-) 1233 WP_041235357.1 CCA tRNA nucleotidyltransferase -

Sequence

Protein


Download         Length: 672 a.a.        Molecular weight: 75447.01 Da        Isoelectric Point: 4.8867

>NTDB_id=55068 DACSA_RS06005 WP_015228894.1 1191377..1193395(-) (pilB/pilB1) [Dactylococcopsis salina PCC 8305]
MTYSSSAKKRQRALTTLRDSSPFGSKLIQAGYADSKQIQEARQEVSQSEGKRSFTEALQSVTGKELPPELYRQYKKHHLF
ELKIYYGVESLDPEVTNLDQYPIKELIDDVIPVDMCRRYKLLPLDKKDTRPPSLLVGMVNPDQLDAQDNLMRILRPKQLK
LQRIVMTLEDYQRIIGEYLDEQSAQEEEQAADEQLDVTKDLANLDVDGLEEVNDEAEADLEKSLGDAQAAPIINVVNRIL
IKALRDGASDIHIEPQEEYIRVRMRRDGVLQKAFDNLPIKIAPAIAARFKIIADLDIAERRMPQDGKIRRRYQGRNVDFR
MSTLPSRYGEKVVLRILDNSATQLGLDKLITDAESLEVVREMASRPFGLILVTGPTGSGKSTTLYSILAERNDPGVNIST
AEDPIEYSLPGITQVQVIREKGMDFASILRSFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARL
DEMGVEPFMISGSLIGVLAQRLMRRVCSECRIPYNPTQEELARFGLSATKEEEITFHKANCLQPEEIEEARKKGKLCSKC
GGNGYKGRAGVYEIMQMTETLQTLINEGAPTERIKEAGVEEGMKTLLAYSLNLVREGYTTLEEVERVTFTDSGVEAEMKA
KRKSSLTCSTCGAELQQEWLDCPYCLTPRFQD

Nucleotide


Download         Length: 2019 bp        

>NTDB_id=55068 DACSA_RS06005 WP_015228894.1 1191377..1193395(-) (pilB/pilB1) [Dactylococcopsis salina PCC 8305]
ATGACGTATTCTTCATCTGCAAAAAAACGTCAAAGAGCCCTGACCACCCTACGTGACTCCTCGCCGTTTGGAAGTAAATT
AATTCAAGCGGGTTACGCGGATAGTAAACAAATCCAAGAAGCCAGACAAGAGGTTTCCCAATCAGAAGGAAAACGATCGT
TTACTGAAGCGTTGCAATCGGTTACTGGCAAGGAATTACCGCCAGAACTTTATCGTCAATATAAAAAGCATCATCTGTTT
GAACTCAAAATTTATTATGGCGTTGAGTCCCTCGATCCAGAAGTAACGAACTTAGATCAGTATCCCATTAAGGAATTGAT
TGACGATGTAATTCCTGTGGATATGTGTCGCCGCTATAAGTTACTCCCTTTAGATAAGAAAGATACTCGCCCCCCTTCTC
TATTGGTGGGAATGGTGAATCCTGATCAATTGGATGCTCAAGATAATCTAATGCGGATTTTGCGTCCGAAACAGTTAAAA
CTGCAGCGCATTGTAATGACGTTGGAGGATTATCAACGGATCATTGGTGAATACCTAGATGAACAATCTGCTCAAGAGGA
AGAACAAGCCGCAGATGAACAGTTAGATGTGACTAAAGACCTCGCTAATCTGGATGTGGATGGGTTAGAAGAAGTTAATG
ACGAAGCTGAGGCAGATTTAGAAAAGTCTTTGGGGGATGCTCAAGCCGCACCGATTATTAATGTGGTGAATCGGATTTTA
ATTAAGGCGCTTCGGGATGGTGCTTCTGATATTCATATTGAACCCCAAGAAGAGTATATTCGGGTGCGGATGCGTCGAGA
TGGGGTGCTACAAAAAGCCTTTGATAATCTCCCGATTAAAATTGCCCCTGCGATCGCGGCGCGTTTTAAGATTATAGCGG
ACTTGGATATTGCAGAACGCAGAATGCCTCAAGACGGGAAAATTCGTCGGCGGTATCAAGGGCGAAATGTGGATTTTCGG
ATGAGTACCTTACCGAGTCGCTATGGGGAGAAAGTGGTACTACGGATTTTGGATAATAGTGCCACACAGTTGGGATTAGA
CAAACTGATTACTGATGCAGAAAGTCTAGAAGTGGTTAGGGAAATGGCAAGTCGTCCTTTTGGCTTGATTTTAGTGACGG
GACCCACTGGCTCAGGGAAATCAACGACACTCTATTCGATTCTCGCCGAACGCAATGACCCAGGGGTAAATATTAGCACC
GCCGAAGACCCGATCGAGTATTCTTTACCTGGAATTACCCAAGTGCAGGTGATTCGGGAAAAAGGCATGGATTTCGCGTC
GATTTTACGATCGTTTCTCCGTCAAGACCCGGACGTGATTCTGGTGGGGGAAACGCGAGATAAAGAAACGGCAAAAACTG
CGATCGAGGCTGCCTTAACAGGACACTTAGTTTTAACCACCTTACACACCAACGATGCTGCTGGCGCGATCGCGCGGTTA
GACGAAATGGGAGTCGAACCCTTTATGATTTCAGGCTCATTAATCGGTGTTCTCGCTCAACGTTTAATGCGTCGAGTGTG
CAGTGAGTGTCGCATCCCTTATAATCCCACGCAAGAGGAACTCGCTCGTTTTGGCTTATCCGCAACTAAAGAAGAAGAAA
TCACCTTTCATAAAGCCAACTGTCTGCAACCCGAAGAAATAGAAGAAGCTCGCAAAAAAGGAAAACTCTGTTCTAAATGT
GGTGGTAACGGTTACAAAGGACGGGCTGGGGTTTATGAAATCATGCAAATGACCGAAACCCTACAAACCCTAATTAATGA
AGGCGCACCCACCGAACGCATCAAAGAAGCAGGGGTAGAAGAAGGCATGAAAACGCTTCTTGCTTATAGTTTAAATCTGG
TTCGAGAAGGTTATACCACCCTCGAAGAAGTGGAACGAGTGACCTTTACCGATTCTGGAGTCGAAGCAGAAATGAAGGCA
AAACGGAAAAGCTCTCTCACTTGTAGCACTTGCGGGGCGGAATTACAACAAGAATGGCTGGATTGTCCTTATTGTTTAAC
CCCTCGTTTCCAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(238-505)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(27-184)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9YU62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.865

98.958

0.652
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.461

90.476

0.366

Multiple sequence alignment