Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   J5M87_RS08190 Genome accession   NZ_CP072115
Coordinates   1747445..1748764 (-) Length   439 a.a.
NCBI ID   WP_154608921.1    Uniprot ID   A0A6I4RC30
Organism   Streptococcus zhangguiae strain zg-86     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1742445..1753764
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5M87_RS08180 (J5M87_08180) hpf 1746195..1746737 (-) 543 WP_154608923.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  J5M87_RS08185 (J5M87_08185) - 1746814..1747479 (-) 666 WP_154608922.1 ComF family protein -
  J5M87_RS08190 (J5M87_08190) comFA/cflA 1747445..1748764 (-) 1320 WP_154608921.1 DEAD/DEAH box helicase Machinery gene
  J5M87_RS08195 (J5M87_08195) - 1748831..1749463 (+) 633 WP_154608920.1 YigZ family protein -
  J5M87_RS08200 (J5M87_08200) cysK 1749552..1750472 (+) 921 WP_154608919.1 cysteine synthase A -
  J5M87_RS08205 (J5M87_08205) - 1751136..1751501 (-) 366 WP_154608918.1 S1 RNA-binding domain-containing protein -
  J5M87_RS08210 (J5M87_08210) - 1751503..1752903 (-) 1401 WP_154608917.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 50017.61 Da        Isoelectric Point: 8.1185

>NTDB_id=550551 J5M87_RS08190 WP_154608921.1 1747445..1748764(-) (comFA/cflA) [Streptococcus zhangguiae strain zg-86]
MADMANYYGRLFTQYQLTETERLQAKTIPSQTPQQTCFRCSTLFEEKHCLPNGALYCRECILLGRVRTDEELYYFPQQPF
PSQHSLKWKGKLTAWQQEISNGLQEQFKARKATMVHAVTGAGKTEMIYQVVADVIDGGGAVCLASPRIDVCIELYKRLKE
DFTCPISLLHGKSDSYFRTPLVISTTHQLLKFYRAFDLILIDEVDAFPYVDNPMLYCAVENAVKDDGVTMFLTATSTDEL
EEKVRRGALHRLQLPRRFHGNPLVVPQKVWLSSFETHLNKKKISPKLTGMIAEQRKTRFPLLIFLPEIRTGQLFSQVLAE
YFPNQCIGFVSSQTENRLEIVEQFRKREIDILVTTTILERGVTFPCVDVFVVQANHALYTSSSLIQIAGRVGRSLERPTG
TLLFFHDGSNRAIEKAIREIRLMNKEAGYDNVSTVSATS

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=550551 J5M87_RS08190 WP_154608921.1 1747445..1748764(-) (comFA/cflA) [Streptococcus zhangguiae strain zg-86]
ATGGCAGATATGGCAAATTACTATGGCAGATTGTTTACCCAGTATCAATTGACCGAAACAGAACGACTGCAAGCTAAAAC
GATACCAAGTCAAACACCGCAACAGACTTGTTTTCGCTGTTCAACCTTATTTGAAGAAAAGCACTGCTTACCAAATGGAG
CTCTGTACTGTCGAGAATGTATACTATTGGGACGTGTACGGACAGATGAAGAGTTGTACTATTTTCCTCAGCAACCATTT
CCTTCTCAGCACTCTCTCAAGTGGAAGGGGAAATTAACCGCTTGGCAACAAGAAATTTCAAATGGTCTGCAGGAGCAGTT
TAAAGCAAGAAAAGCCACAATGGTACATGCTGTGACGGGAGCTGGTAAAACAGAAATGATTTACCAAGTGGTTGCTGATG
TGATTGATGGAGGGGGAGCTGTTTGCCTAGCTAGTCCTCGGATTGATGTCTGCATCGAATTGTATAAAAGGTTGAAAGAG
GATTTTACTTGTCCAATCAGTCTTTTGCATGGAAAATCGGATTCGTATTTTCGAACGCCTCTAGTTATTTCGACCACGCA
TCAATTGCTCAAATTTTATCGGGCTTTTGATTTGATTTTGATTGACGAAGTGGATGCTTTTCCTTATGTGGACAATCCTA
TGTTGTATTGTGCAGTAGAAAATGCTGTGAAAGATGATGGTGTTACTATGTTTCTAACGGCTACTTCTACAGATGAGTTG
GAAGAAAAGGTTCGTAGAGGAGCCCTGCATCGTCTGCAATTACCTCGCCGTTTCCATGGAAATCCACTAGTTGTTCCACA
AAAAGTGTGGTTATCAAGTTTTGAAACACATTTAAATAAGAAAAAGATTAGTCCAAAACTGACTGGTATGATAGCAGAAC
AGCGGAAAACTAGGTTCCCCTTACTCATATTTTTGCCTGAAATTCGAACAGGACAACTATTTTCACAGGTGTTAGCAGAG
TATTTTCCCAATCAATGTATTGGGTTTGTATCCAGTCAGACTGAAAATCGTTTAGAAATCGTCGAACAATTTCGTAAAAG
AGAGATTGATATTCTTGTTACAACAACGATTCTAGAAAGAGGAGTAACCTTTCCTTGTGTAGATGTTTTTGTTGTACAAG
CTAATCATGCTCTTTACACATCTTCTTCTCTTATTCAGATTGCTGGGAGGGTGGGGCGAAGTCTGGAGAGACCGACAGGG
ACGTTACTCTTTTTTCATGATGGAAGTAATCGTGCGATTGAAAAAGCTATTCGGGAAATTCGACTAATGAACAAGGAGGC
AGGATATGACAATGTGTCTACTGTGTCAGCAACTTCTTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(293-393)

Type III restriction enzyme, res subunit (ResIII)

(91-167)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4RC30

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

64.319

97.039

0.624
  comFA/cflA Streptococcus pneumoniae D39

64.085

97.039

0.622
  comFA/cflA Streptococcus pneumoniae R6

64.085

97.039

0.622
  comFA/cflA Streptococcus pneumoniae TIGR4

64.085

97.039

0.622
  comFA/cflA Streptococcus pneumoniae Rx1

64.085

97.039

0.622
  comFA/cflA Streptococcus mitis SK321

63.615

97.039

0.617
  comFA Lactococcus lactis subsp. cremoris KW2

53.046

89.749

0.476
  comFA Latilactobacillus sakei subsp. sakei 23K

37.587

98.178

0.369