Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   J5Z97_RS06745 Genome accession   NZ_CP072112
Coordinates   1330463..1331788 (-) Length   441 a.a.
NCBI ID   WP_023611640.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain M08500     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1325463..1336788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5Z97_RS06735 (J5Z97_06735) raiA 1329198..1329746 (-) 549 WP_002988974.1 ribosome-associated translation inhibitor RaiA -
  J5Z97_RS06740 (J5Z97_06740) - 1329826..1330491 (-) 666 WP_002983685.1 ComF family protein -
  J5Z97_RS06745 (J5Z97_06745) comFA/cflA 1330463..1331788 (-) 1326 WP_023611640.1 DEAD/DEAH box helicase Machinery gene
  J5Z97_RS06750 (J5Z97_06750) - 1331844..1332476 (+) 633 WP_002988970.1 YigZ family protein -
  J5Z97_RS06755 (J5Z97_06755) cysK 1332604..1333545 (+) 942 WP_002983675.1 cysteine synthase A -
  J5Z97_RS06760 (J5Z97_06760) - 1333563..1333940 (-) 378 WP_002983674.1 S1 RNA-binding domain-containing protein -
  J5Z97_RS06765 (J5Z97_06765) - 1333940..1335340 (-) 1401 WP_023611641.1 Cof-type HAD-IIB family hydrolase -
  J5Z97_RS06770 (J5Z97_06770) - 1335377..1336018 (-) 642 WP_002983671.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49820.30 Da        Isoelectric Point: 10.0084

>NTDB_id=550488 J5Z97_RS06745 WP_023611640.1 1330463..1331788(-) (comFA/cflA) [Streptococcus pyogenes strain M08500]
MESIENSYGRLLLESQLPDSARQLAQPLKSVVISRGKMICQRCHYQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPP
MHFPKGNYLVWSGQLTAYQEMISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTILMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=550488 J5Z97_RS06745 WP_023611640.1 1330463..1331788(-) (comFA/cflA) [Streptococcus pyogenes strain M08500]
ATGGAGTCTATTGAAAATAGTTATGGGCGGTTGTTGCTAGAAAGCCAGTTACCAGATTCAGCAAGGCAACTTGCTCAACC
TTTAAAAAGTGTAGTAATATCGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAGTGGAGCATATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGAGCGGTCAACTAACTGCTTATCAAGAGATGATTTCCCAACAGTTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTCTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCAGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTGAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGCCATTAGT
TATTCCGAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCTATCCCCTTTTAATTTTTCTACCCACTATTCTAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACAACAATTTTAGAAAGGGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAGAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTACTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(289-396)

DEAD/DEAH box helicase (DEAD)

(98-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae D39

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae R6

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae TIGR4

54.415

95.011

0.517
  comFA/cflA Streptococcus mitis NCTC 12261

53.081

95.692

0.508
  comFA/cflA Streptococcus mitis SK321

52.133

95.692

0.499
  comFA Lactococcus lactis subsp. cremoris KW2

47.368

90.476

0.429
  comFA Latilactobacillus sakei subsp. sakei 23K

39.851

91.61

0.365