Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ANACY_RS21510 Genome accession   NC_019771
Coordinates   4908071..4910083 (-) Length   670 a.a.
NCBI ID   WP_015216329.1    Uniprot ID   A0A9X1GKC3
Organism   Anabaena cylindrica PCC 7122     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4903071..4915083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ANACY_RS21485 (Anacy_4346) - 4903435..4903704 (-) 270 WP_015216324.1 hypothetical protein -
  ANACY_RS21490 (Anacy_4347) - 4903821..4904816 (+) 996 WP_015216325.1 hypothetical protein -
  ANACY_RS21495 (Anacy_4348) - 4904824..4905384 (-) 561 WP_015216326.1 hypothetical protein -
  ANACY_RS21500 (Anacy_4349) - 4905457..4906680 (-) 1224 WP_015216327.1 type II secretion system F family protein -
  ANACY_RS21505 (Anacy_4350) pilT 4906838..4907962 (-) 1125 WP_015216328.1 type IV pilus twitching motility protein PilT Machinery gene
  ANACY_RS21510 (Anacy_4351) pilB/pilB1 4908071..4910083 (-) 2013 WP_015216329.1 GspE/PulE family protein Machinery gene
  ANACY_RS21515 (Anacy_4352) grpE 4910766..4911512 (+) 747 WP_015216330.1 nucleotide exchange factor GrpE -
  ANACY_RS21520 (Anacy_4353) dnaK 4911802..4913781 (+) 1980 WP_015216331.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 75419.26 Da        Isoelectric Point: 4.9269

>NTDB_id=55018 ANACY_RS21510 WP_015216329.1 4908071..4910083(-) (pilB/pilB1) [Anabaena cylindrica PCC 7122]
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYINSEQMKQALIESRKSGKMLTDVLESITGQQLSPEFLREYKKQHLFELK
ILYGVEFLDPEVNQIGDLMVSQLIETLIPVDICRRHRLVPLSRHDEKTPPYVLVAMVDPDNLEASDDLNRILRNQNLSLQ
RMVITQEDYQQLINQYLDEFAVKQKQLEQEKFTDVGQDLVDIENLNDEEVAEDKDADINASIKDAEDAPIIKLVNRILFK
ALHEGVSDIHIEPQEENLRIRFRKDGVLREAFPAMPKKIILAVTARFKIIANLDIAERRLPQDGRIRRLFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLDKLITDPETLHIVQDMVSRPFGLILVTGPTGSGKTTSLYSALAEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFSTALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MEIEPFMVSSSLIGVLAQRLVRRVCSECRIPYTPTTEELARYGLSASTDVGITFYKANSLLPEAIAEAQSKNQLCQKCNG
VGYKGRVGVYEVMRITETLQTLINEDAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVDRVTFTDTGLEAELKAKR
KSGLTCKTCDATLQQEWLDCPYCMTPRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=55018 ANACY_RS21510 WP_015216329.1 4908071..4910083(-) (pilB/pilB1) [Anabaena cylindrica PCC 7122]
ATGACTTACTCGTCACCACAACGGCGTAGCACCGCTCTAACTACAAGAACAGAGTTTTCGCCCTTCGGCAACAAGCTAGT
ACAATCTGGCTATATCAACAGTGAACAGATGAAGCAGGCGCTGATTGAAAGCCGCAAGTCTGGCAAAATGTTGACAGATG
TACTAGAGTCAATCACTGGGCAACAACTATCACCTGAATTTCTCAGGGAATACAAAAAACAACATTTATTTGAACTCAAA
ATACTATACGGTGTTGAATTCCTAGATCCTGAAGTTAATCAAATTGGCGACCTGATGGTGAGTCAATTGATTGAAACCTT
GATTCCAGTGGATATCTGTCGTCGTCATCGTCTAGTACCTCTGTCGAGACACGATGAAAAAACCCCGCCTTATGTGTTAG
TGGCAATGGTCGATCCGGATAATCTAGAGGCATCTGATGACCTCAACCGGATTTTGCGTAACCAAAATTTGTCCTTACAG
AGGATGGTAATTACTCAGGAAGATTACCAACAACTGATTAACCAATACTTAGATGAGTTTGCTGTTAAACAAAAGCAACT
CGAACAAGAAAAGTTTACAGATGTTGGTCAAGATTTAGTAGATATCGAAAATCTGAATGATGAAGAAGTCGCTGAGGATA
AGGATGCGGACATAAACGCATCAATTAAGGATGCTGAAGATGCACCTATTATCAAACTTGTCAACAGAATCCTGTTCAAA
GCTTTGCATGAAGGTGTTTCGGATATTCACATCGAACCACAAGAGGAAAACTTAAGAATTCGCTTCCGTAAAGATGGGGT
GCTGCGTGAAGCTTTTCCCGCTATGCCCAAAAAAATTATTTTGGCGGTGACAGCCCGATTTAAAATCATTGCTAATTTAG
ATATCGCTGAGAGACGCTTACCCCAAGATGGACGCATTCGCAGATTGTTTGAAGGCAGGAAAGTAGACTTCCGCGTCAAT
ACGTTACCCAGTCGTTATGGGGAAAAGGTGGTACTACGGATTTTGGATAACTCATCAACTCAACTGGGATTGGATAAGTT
AATTACCGATCCAGAGACTTTGCATATTGTCCAGGATATGGTCAGTAGGCCTTTCGGGTTGATTTTGGTGACAGGTCCGA
CTGGTTCTGGTAAAACGACTTCGTTGTATTCGGCATTGGCAGAAAAGAATGATCCGGGGATTAATATCAGTACTGTAGAA
GACCCGATTGAGTACAGTTTGCCAGGGATTACGCAAGTACAGGTAATACGAGAAAAAGGACTAGATTTTTCAACAGCTTT
AAGAGCCTTCTTACGACAAGATCCTGATGTGTTATTGGTGGGTGAAACACGGGATAAAGAAACGGCGAAAACAGCGATTG
AAGCGGCTTTGACAGGTCACTTGGTTTTAACTACCTTACACACAAATGATGCTCCTGGTGCGATCGCTCGTTTGGGAGAA
ATGGAAATCGAACCATTCATGGTTTCCAGTTCCCTAATTGGTGTGTTAGCACAACGTCTAGTCCGTCGCGTCTGTTCAGA
GTGTCGGATTCCCTATACTCCCACTACCGAAGAACTCGCTCGTTATGGGTTATCAGCTTCTACAGATGTGGGCATAACCT
TTTATAAAGCTAACTCACTACTTCCAGAAGCGATCGCAGAAGCTCAAAGCAAAAATCAGCTTTGCCAAAAATGTAATGGT
GTTGGCTACAAGGGACGTGTTGGTGTTTATGAAGTCATGCGGATCACCGAAACCCTCCAAACTCTCATCAACGAAGATGC
ACCCACAGAACGCATTAAAGAAGTAGCAGTAGAAGAAGGTATGAAAACCTTACTCGCCTACAGTCTCGATTTAGTACGTC
AAGGTGCCACCACTCTAGAAGAAGTTGATCGAGTGACATTCACTGATACAGGTTTGGAAGCTGAATTGAAAGCAAAACGC
AAGAGCGGTCTCACCTGTAAAACTTGTGATGCCACACTACAACAAGAATGGCTAGATTGTCCTTACTGTATGACACCTCG
TTTTCAAGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-178)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.625

100

0.658

Multiple sequence alignment