Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LSG45_RS12035 Genome accession   NZ_CP089238
Coordinates   2542467..2544194 (+) Length   575 a.a.
NCBI ID   WP_023106441.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain JNQH-PA033     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2537467..2549194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSG45_RS12015 (LSG45_12015) - 2538524..2539276 (+) 753 WP_234575640.1 class I SAM-dependent methyltransferase -
  LSG45_RS12020 (LSG45_12020) - 2539300..2540090 (-) 791 Protein_2378 alpha/beta fold hydrolase -
  LSG45_RS12025 (LSG45_12025) - 2540268..2541230 (+) 963 WP_224789405.1 GlxA family transcriptional regulator -
  LSG45_RS12030 (LSG45_12030) - 2541674..2542477 (+) 804 WP_003131067.1 ABC transporter permease -
  LSG45_RS12035 (LSG45_12035) pilF 2542467..2544194 (+) 1728 WP_023106441.1 GspE/PulE family protein Machinery gene
  LSG45_RS12040 (LSG45_12040) - 2544199..2545386 (+) 1188 WP_003090520.1 type II secretion system F family protein -
  LSG45_RS12045 (LSG45_12045) gspG 2545440..2545874 (+) 435 WP_023435131.1 type II secretion system major pseudopilin GspG -
  LSG45_RS12050 (LSG45_12050) - 2545843..2546253 (+) 411 WP_003110088.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LSG45_RS12055 (LSG45_12055) - 2546253..2546678 (+) 426 WP_003116709.1 type II secretion system protein J -
  LSG45_RS12060 (LSG45_12060) - 2546675..2547265 (+) 591 WP_016852732.1 type II secretion system protein J -
  LSG45_RS12065 (LSG45_12065) - 2547255..2548334 (+) 1080 WP_234575641.1 general secretion pathway protein GspK -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63627.68 Da        Isoelectric Point: 5.2400

>NTDB_id=549792 LSG45_RS12035 WP_023106441.1 2542467..2544194(+) (pilF) [Pseudomonas aeruginosa strain JNQH-PA033]
MSSEAVSPGPQVTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGADL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSTSIRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LARQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLRLDRLGMLPDHLQRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLISVEDPVEYQVPNITQVQVHADIGLTFAAALRSILRQDPDVIMIGEI
RDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAA
TEAATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAW
LGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=549792 LSG45_RS12035 WP_023106441.1 2542467..2544194(+) (pilF) [Pseudomonas aeruginosa strain JNQH-PA033]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGGTGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGTCTGGGAGGCATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGCTGGTGGGGGCCGACCTG
CGGAAACCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGCACCCCGATCAATACCGACTGGTTCGTCGAAGAACAGCT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCGCGTGATCCGTTATCTACTTCGATACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCAGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCATTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCACGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGCTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGCTCTTCGGATATTCACATCGAACCCCGGGAGT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTC
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTAGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTCATGCGCCTATTGCCGA
AGGAGCGTGAGGGGTTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCAGAGGATGCGTGCATGGGTAGCGGAA
CCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCCACGACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTGGAATACCAGGTGCCCAACATCACCCAGGTCCAGGTCCATG
CCGACATCGGCCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATA
CGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGA
TGCGGTGAGTGCCTTCACGCGCTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGG
CCCAGCGACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG
ACCGAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTGCGAAGG
TACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGAG
CTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGG
TTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(194-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

41.696

98.435

0.41
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.624

98.609

0.381
  pilF Neisseria gonorrhoeae MS11

36.667

99.13

0.363