Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   CRI9333_RS18775 Genome accession   NC_019753
Coordinates   4331331..4333325 (+) Length   664 a.a.
NCBI ID   WP_015204749.1    Uniprot ID   -
Organism   Crinalium epipsammum PCC 9333     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4326331..4338325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRI9333_RS18755 (Cri9333_3836) dnaJ 4326377..4327504 (-) 1128 WP_015204746.1 molecular chaperone DnaJ -
  CRI9333_RS18760 (Cri9333_3837) dnaK 4327596..4329563 (-) 1968 WP_015204747.1 molecular chaperone DnaK -
  CRI9333_RS18765 (Cri9333_3838) grpE 4329720..4330472 (-) 753 WP_015204748.1 nucleotide exchange factor GrpE -
  CRI9333_RS18770 - 4330508..4330696 (+) 189 WP_041226121.1 hypothetical protein -
  CRI9333_RS18775 (Cri9333_3839) pilB/pilB1 4331331..4333325 (+) 1995 WP_015204749.1 GspE/PulE family protein Machinery gene
  CRI9333_RS18780 (Cri9333_3840) pilT 4333418..4334503 (+) 1086 WP_015204750.1 type IV pilus twitching motility protein PilT Machinery gene
  CRI9333_RS18785 (Cri9333_3841) - 4334618..4335844 (+) 1227 WP_015204751.1 type II secretion system F family protein -
  CRI9333_RS18790 (Cri9333_3842) rlmN 4335949..4337019 (+) 1071 WP_015204752.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  CRI9333_RS18795 (Cri9333_3843) - 4337034..4337234 (-) 201 WP_015204753.1 hypothetical protein -

Sequence

Protein


Download         Length: 664 a.a.        Molecular weight: 74096.70 Da        Isoelectric Point: 5.1786

>NTDB_id=54966 CRI9333_RS18775 WP_015204749.1 4331331..4333325(+) (pilB/pilB1) [Crinalium epipsammum PCC 9333]
MTQSSSSRRALVVRNASHDLSPFASKLVQLGYIDSEKMQQAALESRKTGKPLTQILESLTGKALSPELQRQYKKNQLFEL
KIIYGVESLDPEIKQIEQSQVSELIDTLIPIDSCRRSRFVPLSKEATEPPSVLVAMVNPDDLDAQDDLNRILRPQGINWQ
RMVITAEDYSSIINQYLDQQAKKDEAAKAQKSFDVQGEIQNLEGLEAEDSHDIPDEDLTAAAEDAPIITLVNKILAKALT
DEVSDIHVEPQEEYLRIRFRKDGVLREAFDPLPKKIIPAVTSRFKIIAQLDIAERRAPQDGRIRRVFQGRKVDFRVNTLP
SRYGEKICMRILDNSSTQLGLDKLISDPDALQSVRDMASRPFGMILVTGPTGSGKSTTLYSILAERNSPDVNISTAEDPI
EYALPGINQVQVIREKGMNFAAILRAFMRQDPDVILVGETRDVETAKTAIEAALTGHLVMTTLHANDASSSIARLDEMGV
EPFMVAASLLGVCAQRLMRRVCTECRIPYQPTIEELNRYGLSASKEGSVTFYKANSLQPEEIKTATNLCPKCKGLGYKGR
VGVYEVMRITERLQSLINKGGTTDMIKEAAVEDGMVTLLSYSLNLVREGHTTLEEVERVTFTDTGLESELKAKRKTTLTC
RICSAGLKQEWLDCPYCMTSRFEE

Nucleotide


Download         Length: 1995 bp        

>NTDB_id=54966 CRI9333_RS18775 WP_015204749.1 4331331..4333325(+) (pilB/pilB1) [Crinalium epipsammum PCC 9333]
ATGACTCAATCCTCATCATCTCGGCGTGCTTTAGTTGTTCGCAATGCCAGCCATGATTTGTCGCCCTTTGCCAGCAAGCT
AGTTCAGTTAGGCTACATCGACTCCGAAAAGATGCAGCAGGCGGCGCTTGAAAGTCGCAAAACTGGAAAACCCCTAACAC
AAATCCTAGAGTCGCTCACAGGAAAGGCGCTATCTCCTGAGTTACAGCGTCAGTATAAAAAGAATCAACTGTTTGAACTA
AAGATTATATACGGCGTTGAATCTTTAGATCCGGAAATTAAACAAATTGAACAAAGTCAGGTTTCTGAGTTGATCGATAC
ACTCATCCCTATTGATAGTTGTCGTCGCTCCCGCTTCGTACCTCTATCAAAAGAAGCAACTGAGCCACCATCTGTCTTAG
TGGCAATGGTTAACCCTGATGATTTAGATGCCCAAGATGATCTAAACCGAATTTTACGCCCCCAGGGAATTAATTGGCAA
AGGATGGTAATCACCGCCGAAGACTATTCCTCAATTATTAACCAATACCTAGATCAGCAAGCAAAGAAAGACGAAGCCGC
TAAAGCTCAAAAGTCTTTTGATGTTCAGGGAGAAATCCAAAATCTTGAAGGACTTGAAGCCGAAGACTCCCATGATATTC
CTGATGAAGATTTAACGGCAGCAGCAGAAGATGCCCCGATTATCACCCTGGTCAATAAAATTTTGGCTAAGGCATTAACT
GATGAAGTTTCTGATATTCACGTTGAACCTCAAGAAGAATATTTACGCATTCGCTTTCGCAAAGATGGGGTACTCAGGGA
GGCATTTGATCCTCTACCGAAAAAGATTATTCCAGCAGTTACTTCACGTTTCAAAATCATTGCACAACTAGACATTGCCG
AACGACGCGCACCTCAAGATGGTCGTATCCGGCGAGTGTTTCAAGGACGTAAAGTTGATTTCCGTGTAAACACTTTACCT
AGTCGCTATGGCGAGAAAATTTGTATGCGGATTTTGGATAACTCTTCCACCCAGTTGGGCTTGGATAAGTTGATTAGCGA
TCCAGATGCGCTACAAAGTGTCAGAGACATGGCTAGTCGTCCTTTTGGGATGATTTTGGTGACAGGTCCAACAGGATCTG
GTAAATCAACAACACTATATTCAATTTTGGCTGAACGCAACAGTCCTGATGTGAACATTAGTACGGCAGAAGACCCGATT
GAATATGCCTTACCAGGAATTAATCAGGTGCAGGTGATTCGCGAAAAAGGCATGAACTTTGCCGCAATTTTACGAGCATT
CATGCGCCAAGACCCCGATGTAATTCTAGTAGGTGAAACGCGAGATGTAGAAACAGCAAAAACCGCAATTGAAGCTGCTT
TAACTGGACACTTGGTAATGACGACTCTGCACGCTAATGATGCTTCAAGTTCAATTGCACGGTTAGATGAAATGGGCGTG
GAACCCTTCATGGTGGCAGCCTCATTGCTTGGCGTTTGCGCCCAAAGGTTGATGCGACGAGTATGCACTGAGTGCCGTAT
TCCCTATCAACCGACTATTGAGGAGCTTAACCGATATGGTTTATCAGCTTCCAAGGAAGGCTCTGTTACGTTTTACAAAG
CCAACTCCTTACAACCTGAAGAAATTAAAACTGCGACAAATCTCTGCCCAAAATGTAAGGGTCTTGGTTATAAAGGGCGT
GTAGGTGTCTATGAAGTTATGCGTATAACTGAGCGGTTGCAAAGCTTAATCAACAAAGGTGGCACGACCGACATGATTAA
GGAAGCGGCTGTGGAAGACGGGATGGTGACATTGTTATCTTACAGCTTAAATTTAGTGCGTGAAGGACACACAACCCTGG
AAGAAGTTGAGCGGGTGACTTTTACGGATACAGGTTTAGAATCAGAATTAAAAGCAAAACGTAAGACTACTTTAACTTGT
CGCATTTGTAGTGCTGGATTAAAACAAGAGTGGCTTGATTGTCCCTACTGCATGACATCTCGTTTTGAAGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(233-500)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(27-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.416

100

0.664

Multiple sequence alignment