Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   STA7437_RS00755 Genome accession   NC_019748
Coordinates   171377..173380 (+) Length   667 a.a.
NCBI ID   WP_015191448.1    Uniprot ID   K9XME8
Organism   Stanieria cyanosphaera PCC 7437     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 166377..178380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STA7437_RS00735 (Sta7437_0153) - 166555..166839 (-) 285 WP_015191444.1 sulfurtransferase TusA family protein -
  STA7437_RS00740 (Sta7437_0154) dnaJ 166836..167966 (-) 1131 WP_015191445.1 molecular chaperone DnaJ -
  STA7437_RS00745 (Sta7437_0155) dnaK 168070..170082 (-) 2013 WP_015191446.1 molecular chaperone DnaK -
  STA7437_RS00750 (Sta7437_0156) grpE 170237..171016 (-) 780 WP_015191447.1 nucleotide exchange factor GrpE -
  STA7437_RS00755 (Sta7437_0157) pilB/pilB1 171377..173380 (+) 2004 WP_015191448.1 GspE/PulE family protein Machinery gene
  STA7437_RS00760 (Sta7437_0158) pilT 173404..174498 (+) 1095 WP_015191449.1 type IV pilus twitching motility protein PilT Machinery gene
  STA7437_RS00765 (Sta7437_0159) - 174555..175769 (+) 1215 WP_015191450.1 type II secretion system F family protein -
  STA7437_RS00770 (Sta7437_0160) - 175781..176350 (+) 570 WP_015191451.1 hypothetical protein -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 74487.09 Da        Isoelectric Point: 5.1016

>NTDB_id=54919 STA7437_RS00755 WP_015191448.1 171377..173380(+) (pilB/pilB1) [Stanieria cyanosphaera PCC 7437]
MTYSSSSKRTRAIALRNYFSPFGNKLIQVGFVNGDQMQQALSQSRTSGQSLIDVLESLTGQQLPPDLVRQYKKHHLFELK
VLYGVECLDPEISPINNNQMSHLIDTLIPIDICRRYQLIPLQREDKQPPVLLVAMVDPENLEAQDDLNRILRPKGIELQR
MVITPTDYQRLIDQYLDEQVKKEETKKSEAKVDVSINLENLDFNLEETADEVEDDLGAANDAQGAPIISLVNKILARALQ
DGISDIHVEPQEEFLRVRYRKDGVLQQPFDPLPKKITPAVVARFKIMADLDIAEKRLPQDGKIRRMFQGRKVDFRVSSLP
SRYGEKVCLRILDNSATQLGLDKLISDESTLAIVRELAARPFGLILVTGPTGSGKSTTLYSILAERNNPGVNISTAEDPI
EYSLDGITQVQVIREKGMNFASILRAFLRQDPDVILVGETRDPETAKTAIEAALTGHLVITTLHTNDAAGAIARLDEMGI
EPFMISGGLLGVVAQRLMRRVCGECRIAYNPTHEELARFGLSASNEEEITFYKANTLNPEQINEARANGTLCPKCTGIGY
KGRVGVYEVMRNSERLENLINTGATTDRIKEVAVEDGMVTLLAYSLNLVRQGHTTLEEVERVTFTDSGLEAELKAKRKSG
LICRTCNAELKPEWLDCPYCTTSRFSD

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=54919 STA7437_RS00755 WP_015191448.1 171377..173380(+) (pilB/pilB1) [Stanieria cyanosphaera PCC 7437]
ATGACTTATTCTTCATCTTCAAAACGAACTAGAGCGATCGCATTACGCAATTACTTTTCTCCTTTTGGTAATAAGTTAAT
TCAAGTCGGCTTTGTAAATGGAGATCAGATGCAGCAGGCTTTATCACAAAGTCGCACGTCTGGGCAATCGCTAATTGATG
TGCTGGAATCGCTTACTGGTCAACAATTACCTCCTGATTTAGTACGTCAGTATAAAAAACACCATTTATTTGAACTAAAG
GTTTTATATGGGGTTGAATGCTTAGATCCTGAAATCAGTCCAATCAATAATAATCAGATGAGTCATTTAATTGATACTCT
GATTCCGATTGATATTTGTCGTCGCTATCAATTAATCCCTCTACAAAGAGAGGACAAACAACCTCCTGTTTTATTGGTAG
CAATGGTTGATCCCGAAAACTTAGAAGCTCAAGATGATCTCAACCGCATCTTACGTCCTAAAGGAATTGAGCTACAAAGG
ATGGTAATCACTCCGACAGATTATCAAAGACTGATCGATCAATATTTAGACGAGCAAGTTAAAAAAGAAGAAACTAAAAA
ATCTGAAGCGAAAGTTGATGTTTCAATCAATTTAGAAAATTTAGATTTTAATTTAGAAGAAACAGCCGATGAAGTAGAAG
ACGATTTAGGGGCTGCTAATGATGCTCAAGGCGCACCAATCATTAGTTTAGTTAATAAAATTTTGGCTAGAGCTTTACAA
GATGGTATTTCTGATATTCATGTCGAACCTCAAGAAGAATTTTTACGGGTTCGTTATCGCAAAGACGGAGTATTACAACA
ACCATTCGATCCTCTTCCCAAAAAAATTACGCCCGCAGTAGTTGCTCGTTTCAAAATCATGGCAGATTTAGATATTGCTG
AAAAACGTTTACCTCAAGATGGTAAGATTCGGCGAATGTTTCAGGGACGCAAAGTAGATTTTCGGGTTAGTAGTTTACCT
AGTCGCTATGGAGAAAAAGTTTGTTTACGAATTCTAGATAACTCTGCCACTCAGTTAGGTTTAGATAAATTAATTAGTGA
TGAGTCTACTTTAGCCATAGTTAGGGAGTTAGCTGCTCGTCCTTTTGGCTTAATTTTAGTAACAGGGCCTACGGGTTCGG
GTAAATCTACCACTCTTTATTCAATTCTGGCAGAACGAAATAATCCTGGCGTTAATATTAGTACGGCAGAAGATCCTATT
GAATATTCTTTGGATGGGATTACTCAGGTACAGGTAATTCGTGAGAAAGGCATGAATTTTGCCTCGATCTTAAGAGCTTT
CCTTCGACAAGACCCTGATGTCATTCTAGTAGGTGAGACTAGAGATCCTGAAACAGCCAAAACAGCAATTGAAGCAGCGT
TGACTGGTCACTTAGTTATTACAACTCTTCACACTAATGACGCAGCAGGCGCGATCGCTCGTTTGGATGAAATGGGTATT
GAACCTTTTATGATTTCTGGCGGTTTATTGGGAGTAGTAGCTCAAAGATTAATGCGTCGAGTTTGTGGTGAATGTCGGAT
TGCTTACAATCCTACTCATGAAGAATTAGCCAGATTTGGTTTATCTGCCAGCAATGAAGAAGAAATAACTTTTTATAAAG
CTAACACTTTAAACCCAGAACAAATAAACGAAGCAAGAGCTAATGGCACTCTTTGTCCTAAGTGTACTGGTATTGGTTAC
AAAGGCAGGGTTGGTGTTTACGAAGTAATGCGTAATTCTGAGCGCTTAGAGAATTTAATTAACACTGGAGCAACAACCGA
CCGCATCAAGGAAGTTGCTGTTGAAGATGGCATGGTTACCTTACTGGCATATAGTCTAAATTTAGTTCGTCAAGGTCATA
CAACTCTAGAGGAAGTCGAACGCGTAACCTTCACTGATTCTGGTTTGGAAGCAGAGTTAAAAGCCAAACGCAAGAGCGGT
TTAATTTGTCGTACTTGTAATGCAGAGTTAAAACCCGAATGGCTAGATTGTCCCTACTGTACAACATCTAGATTTAGCGA
TTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-179)

Type II/IV secretion system protein (T2SSE)

(233-500)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9XME8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

69.539

100

0.702
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.527

91.004

0.369

Multiple sequence alignment