Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LSP16_RS24020 Genome accession   NZ_CP089067
Coordinates   5105224..5106951 (-) Length   575 a.a.
NCBI ID   WP_219832520.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain UNC_PaerCF19     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5100224..5111951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSP16_RS23990 (LSP16_23880) - 5101084..5102163 (-) 1080 WP_003119955.1 general secretion pathway protein GspK -
  LSP16_RS23995 (LSP16_23885) - 5102153..5102743 (-) 591 WP_003113392.1 type II secretion system protein J -
  LSP16_RS24000 (LSP16_23890) - 5102740..5103165 (-) 426 WP_003097721.1 type II secretion system protein J -
  LSP16_RS24005 (LSP16_23895) - 5103165..5103575 (-) 411 WP_003097723.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LSP16_RS24010 (LSP16_23900) gspG 5103544..5103978 (-) 435 WP_003110089.1 type II secretion system major pseudopilin GspG -
  LSP16_RS24015 (LSP16_23905) - 5104032..5105219 (-) 1188 WP_003097729.1 type II secretion system F family protein -
  LSP16_RS24020 (LSP16_23910) pilF 5105224..5106951 (-) 1728 WP_219832520.1 GspE/PulE family protein Machinery gene
  LSP16_RS24025 (LSP16_23915) - 5106941..5107744 (-) 804 WP_003097732.1 ABC transporter permease -
  LSP16_RS24030 (LSP16_23920) - 5108336..5109088 (-) 753 WP_003116707.1 class I SAM-dependent methyltransferase -
  LSP16_RS24035 (LSP16_23925) - 5109314..5110288 (+) 975 WP_219832521.1 quinone oxidoreductase -
  LSP16_RS24040 (LSP16_23930) - 5110311..5111240 (+) 930 WP_003116706.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63696.81 Da        Isoelectric Point: 5.3299

>NTDB_id=548690 LSP16_RS24020 WP_219832520.1 5105224..5106951(-) (pilF) [Pseudomonas aeruginosa strain UNC_PaerCF19]
MSSEAVSPGPQMTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGDDL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSIRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LARQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLRLDRLGMLPDHLRRMRAWVAE
PHGIILVTGPAGSGKSTTLYGALEEINDGVRKLISVEDPVEYQVPNITQVQVHADIGLTFAAALRSILRQDPDVIMIGEI
RDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAA
TQAATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAW
LGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=548690 LSP16_RS24020 WP_219832520.1 5105224..5106951(-) (pilF) [Pseudomonas aeruginosa strain UNC_PaerCF19]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGATGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGTCTGGGAGGCATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGCTGGTGGGGGACGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGTACCCCGATCAATACCGACTGGTTCGTCGAAGAGCAACT
GGTGGTCTGGGAGGAGGGCGAACAACTGCTGTTCGCGGCGCGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCAGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCACTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCACGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGCTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGTTCTTCGGATATTCACATCGAACCCCGGGAGT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTC
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTCATGCGCCTGTTGCCGA
AGGAGCGCGAGGGGTTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCGGAGGATGCGTGCATGGGTAGCGGAA
CCCCACGGCATCATCCTGGTGACCGGCCCCGCCGGATCGGGCAAGTCCACCACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTGGAATACCAGGTGCCCAATATCACCCAGGTCCAGGTCCATG
CCGACATCGGCCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATA
CGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGA
TGCGGTGAGCGCCTTCACGCGTTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGG
CCCAGCGACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG
ACCCAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTGCGAAGG
TACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGAG
CTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGG
TTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)

Type II/IV secretion system protein (T2SSE)

(194-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

41.343

98.435

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.624

98.609

0.381
  pilF Neisseria gonorrhoeae MS11

36.667

99.13

0.363